• 规格：10μl质粒
• 启动子：Lac promoter，T3 promoter
• 复制子：p15A ori
• 质粒分类：基因文库质粒；其它基因质粒；其它种源质粒
• 质粒大小：5198bp
• 原核抗性：卡那霉素Kan（50μg/ml）
• 克隆菌株：DH5α等大肠杆菌
• 备注：低拷贝
• 质粒简介：             pInt质粒提供提供链霉菌噬菌体phiC31基因，其参考文献：A phage integrase directs efficient site-specific integration in human cells. 
• 质粒图谱1： png/1-1FQ51K30aN.png

质粒序列： 点击查看序列

LOCUS       Exported                5198 bp ds-DNA     circular SYN 15-AUG-2017

KEYWORDS    pInt.dna

FEATURES             Location/Qualifiers

     source          1..5198

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             complement(16..1833)

                     /codon_start=1

                     /product="integrase from phage phi-C31"

                     /label=phage phi-C31 integrase

                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG

                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP

                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL

                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE

                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL

                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA

                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG

                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA

                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE

                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI

                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"

     promoter        complement(1897..1915)

                     /label=T3 promoter

                     /note="promoter for bacteriophage T3 RNA polymerase"

     primer_bind     complement(1936..1952)

                     /label=M13 rev

                     /note="common sequencing primer, one of multiple similar 

                     variants"

     protein_bind    1960..1976

                     /label=lac operator

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="The lac repressor binds to the lac operator to 

                     inhibit transcription in E. coli. This inhibition can be 

                     relieved by adding lactose or 

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     promoter        complement(1984..2014)

                     /label=lac promoter

                     /note="promoter for the E. coli lac operon"

     protein_bind    2029..2050

                     /label=CAP binding site

                     /bound_moiety="E. coli catabolite activator protein"

                     /note="CAP binding activates transcription in the presence 

                     of cAMP."

     rep_origin      2662..3206

                     /direction=RIGHT

                     /label=p15A ori

                     /note="Plasmids containing the medium-copy-number p15A 

                     origin of replication can be propagated in E. coli cells 

                     that contain a second plasmid with the ColE1 origin."

     CDS             3801..4616

                     /codon_start=1

                     /gene="aph(3')-Ia"

                     /product="aminoglycoside phosphotransferase"

                     /label=KanR

                     /note="confers resistance to kanamycin in bacteria or G418 

                     (Geneticin(R)) in eukaryotes"

                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG

                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK

                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA

                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI

                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"

ORIGIN

        1 gatccgggtg tctcgctacg ccgctacgtc ttccgtgccg tcctgggcgt cgtcttcgtc

       61 gtcgtcggtc ggcggcttcg cccacgtgat cgaagcgcgc ttctcgatgg gcgttccctg

      121 ccccctgccc gtagtcgact tcgtgacaac gatcttgtct acgaagagcc cgacgaacac

      181 gcgcttgtcg tctactgacg cgcgccccca ccacgactta gggccggtcg ggtcagcgtc

      241 ggcgtcttcg gggaaccatt ggtcaagggg aagcttcggg gcttcggcgg cttcaagttc

      301 ggcaagccgc tcttccgccc cttgctgccg gagcgtcagc gctgcctgtt gcttccggaa

      361 gtgcttcctg ccaacgggtc cgtcgtacgc gcctgccgcg cggtcttcgt acagctcttc

      421 aagggcgttc agggcgtcgg cgcgctccgc aacaaggttc gcccgttcgc cgctcttctc

      481 aggcgcctca gtgagcttgc cgaagcgtcg ggcggcttcc cacagaagcg ccaacgtctc

      541 ttcgtcgcct tcggcgtgcc tgatcttgtt gaagatgcgt tccgcaacga acttgtcgag

      601 tgccgccatg ctgacgttgc acgtgccttc gtgctgccca ggtgcggacg ggtcgaccac

      661 cttccggcga cggcagcggt aagagtcctt gatcgattct tccccgcgct tcgaagtcat

      721 gacggcgcca cactcgcagt acagcttgtc catggcggac agaatggctt gcccccggga

      781 aagccccttg ccgcgccccc tgccgtccaa ccacgcctga agctcatacc actcagcggg

      841 ctcgatgatc ggtccgcaat caagctcgac cggccggagc gtgatcgggt cgcgctgaat

      901 gcggtaaccc tcaatcttcg tggtcggcgt gccgtccggc ttcttcttgt agatcacctc

      961 agcggcgaag cccgcaatac gcgggtcccg aaggattcgc ataacggttg ccgggtccca

     1021 ggcgcttgaa gcggtcttct tcccaatcgt ctcgccccgg gtcggcacgg cgtcagcgtc

     1081 catgcgctta caaagccccg tgatgctgcc cgggtgaatg gcggcttgac tgcccggctt

     1141 gaagggaagg tgtttgtgcg tcttgatctc acgccaccac caccggatta cgtcgggctc

     1201 gaactcgaag ggtccggtaa ggggagtggt cgagtgcgca agcttgttga tgacgacatt

     1261 gaccattcgg ccgttgcgcg tgatctcctt cgtctccgaa acaagctcga agccgtaagg

     1321 cgccttcccg ccgacgtacc cgcccaattc gcgctgaagg ttcttcgtgt cgagaatctt

     1381 cgccgacttc agcgaagatt ctttgtgcga cgcgtcgagc cgcataatca ggtgaatcag

     1441 gtccatgacg tttccctgcc ggaagacgcc ttcctgagtg gaaacaatcg tcacgcccag

     1501 ggcgagcaat tccgagacaa tcggaatcgc gtccatgacc ttcaggcgcg agaagcgcga

     1561 cacgtcatag acaatgatca tgttgagccg cccggcgcgg cattcgttca ggatgcgttc

     1621 gaactccggg cgctccgccg tcccgaacgc cgacgtgccc ggcgcttcgc tgaaatgccc

     1681 gacgaacctg aaccggcccc cgtcgcgctc gacttcgcgc tgaaggtcgg ccgccttgtc

     1741 ttcgttggcg ctacgctgtg tcgctgggct tgctgcgctc gaattctcgc gctcgcgcga

     1801 ctgacggtcg taagcacccg cgtacgtgtc caccccggtc acaacccctt gtgtcatgtc

     1861 ggcgacccta cgactagtga gctccagctt ttgttccctt tagtgagggt taattgcgcg

     1921 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc

     1981 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta

     2041 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca

     2101 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc

     2161 cgctcggcca aaaaggcctg cagcaatggc aacaacgttg cgcaaactat taactggcga

     2221 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc

     2281 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc

     2341 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg

     2401 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat

     2461 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata

     2521 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct

     2581 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga

     2641 ccccttaata agatgatctt cttgagatcg ttttggtctg cgcgtaatct cttgctctga

     2701 aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt ctctgagcta ccaactcttt

     2761 gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc

     2821 ttaaccggcg catgacttca agactaactc ctctaaatca attaccagtg gctgctgcca

     2881 gtggtgcttt tgcatgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc

     2941 agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc

     3001 cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg

     3061 gtaaaccgaa aggcaggaac aggagagcgc acgagggagc cgccagggga aacgcctggt

     3121 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct

     3181 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt

     3241 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg

     3301 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta

     3361 tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca

     3421 ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag cgctgaggtc

     3481 tgcctcgtga agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc

     3541 agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt

     3601 tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct

     3661 tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat

     3721 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa

     3781 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgaggccgc

     3841 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg

     3901 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc

     3961 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact

     4021 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg

     4081 catggttact caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc

     4141 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga

     4201 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat

     4261 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc

     4321 ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg

     4381 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt

     4441 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga

     4501 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg

     4561 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag

     4621 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcggct

     4681 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa

     4741 cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa

     4801 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg

     4861 gtatgagtca gcaacacctt cttcacgagg cagacctcag cgctcaaaga tgcaggggta

     4921 aaagctaacc gcatctttac cgacaaggca tccggcagtt caacagatcg ggaagggctg

     4981 gatttgctga ggatgaaggt ggaggaaggt gatgtcattc tggtgaagaa gctcgaccgt

     5041 cttggccgcg acaccgccga catgatccaa ctgataaaag agtttgatgc tcagggtgta

     5101 gcggttcggt ttattgacga cgggatcagt accgacggtg atatggggca aatggtggtc

     5161 accatcctgt cggctgtggc acaggctgaa cgccggag

//