• 规格：20μl质粒
• 质粒图谱1： png/1-1F92QF559339.png

质粒序列： 点击查看序列

LOCUS       Exported                2845 bp ds-DNA     circular SYN 28-SEP-2017

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pUC57-Simple-gRNA backbone

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 2845)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Sep 28, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..2845

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        234..252

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     misc_RNA        271..346

                     /label=gRNA scaffold

                     /note="guide RNA scaffold for the Streptococcus pyogenes 

                     CRISPR/Cas9 system"

     promoter        888..992

                     /gene="bla"

                     /label=AmpR promoter

     CDS             993..1853

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      2024..2612

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt

       61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt

      121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc

      181 caagctcgct tgcacgcctc tgcactcgtc ggtcccggca tccgataccc aattaatacg

      241 actcactata ggtcgtcttc gagaagacct gttttagagc tagaaatagc aagttaaaat

      301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt aaagacccaa

      361 tatctcgacg cattcgcgaa gtaccgatct ccaattcact ggccgtcgtt ttacaacgtc

      421 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg

      481 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc

      541 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac

      601 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc

      661 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt

      721 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac

      781 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga

      841 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta

      901 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat

      961 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc

     1021 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga

     1081 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca

     1141 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt

     1201 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg

     1261 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc

     1321 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata

     1381 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt

     1441 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag

     1501 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca

     1561 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg

     1621 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg

     1681 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag

     1741 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg

     1801 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag

     1861 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga

     1921 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt

     1981 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc

     2041 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc

     2101 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac

     2161 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac

     2221 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt

     2281 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct

     2341 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat

     2401 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt

     2461 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg

     2521 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt

     2581 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt

     2641 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg

     2701 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg

     2761 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc

     2821 ccgcgcgttg gccgattcat taatg

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