• 商品货号：FH1236

• 规格：10μl质粒
• 启动子：CMV promoter
• 复制子：ori
• 终止子：bGH poly(A) signal
• 质粒分类：哺乳细胞质粒；哺乳编辑质粒；哺乳Cas9质粒
• 质粒大小：8532bp
• 质粒标签：C-SV40 NLS
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌Stbl3
• 表达宿主：哺乳细胞293T
• 质粒简介：             pCMV-BE3质粒是一个哺乳细胞过表达质粒，CMV启动子驱动BE3（rAPOBEC1-XTEN-Cas9n-UGI-NLS）的表达。pCMV-BE3质粒的参考文献：Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. 
• 质粒图谱1： png/1-1FQ61H4293A.png

质粒序列： 点击查看序列

LOCUS       Exported                8532 bp ds-DNA    circular SYN 16-八月-2017

KEYWORDS    pCMV-BE3

FEATURES             Location/Qualifiers

     source          1..8532

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        132..335

                     /note="CMV promoter"

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     promoter        377..395

                     /note="T7 promoter"

                     /note="promoter for bacteriophage T7 RNA polymerase"

     CDS             409..5541

                     /codon_start=1

                     /note="rAPOBEC1-XTEN-Cas9n-UGI-NLS"

                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG

                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL

                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN

                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI

                     LWATGLKSGSETPGTSESATPESDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGN

                     TDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDS

                     FFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIY

                     LALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSA

                     RLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDD

                     DLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDL

                     TLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLV

                     KLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY

                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP

                     KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDY

                     FKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFE

                     DREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSD

                     GFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVD

                     ELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENT

                     QLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKN

                     RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLV

                     ETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNY

                     HHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYS

                     NIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTE

                     VQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLK

                     SVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASA

                     GELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEF

                     SKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKR

                     YTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSGGSTNLSDIIEKETGKQLVIQESIL

                     MLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIK

                     MLSGGSPKKKRKV"

     CDS             5518..5538

                     /codon_start=1

                     /locus_tag="

                     "

                     /product="nuclear localization signal of SV40 large T 

                     antigen"

                     /note="SV40 NLS"

                     /note="

                     "

                     /protein_id="

                     "

                     /translation="PKKKRKV"

     polyA_signal    5593..5817

                     /note="bGH poly(A) signal"

                     /note="bovine growth hormone polyadenylation signal"

     rep_origin      complement(6290..6878)

                     /direction=LEFT

                     /note="ori"

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(7049..7909)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /note="AmpR"

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(7910..8014)

                     /gene="bla"

                     /note="AmpR promoter"

     enhancer        8284..131

                     /note="CMV enhancer"

                     /note="human cytomegalovirus immediate early enhancer"

ORIGIN

        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg

       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg

      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact

      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa

      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta

      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct

      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gagctcagag

      421 actggcccag tggctgtgga ccccacattg agacggcgga tcgagcccca tgagtttgag

      481 gtattcttcg atccgagaga gctccgcaag gagacctgcc tgctttacga aattaattgg

      541 gggggccggc actccatttg gcgacataca tcacagaaca ctaacaagca cgtcgaagtc

      601 aacttcatcg agaagttcac gacagaaaga tatttctgtc cgaacacaag gtgcagcatt

      661 acctggtttc tcagctggag cccatgcggc gaatgtagta gggccatcac tgaattcctg

      721 tcaaggtatc cccacgtcac tctgtttatt tacatcgcaa ggctgtacca ccacgctgac

      781 ccccgcaatc gacaaggcct gcgggatttg atctcttcag gtgtgactat ccaaattatg

      841 actgagcagg agtcaggata ctgctggaga aactttgtga attatagccc gagtaatgaa

      901 gcccactggc ctaggtatcc ccatctgtgg gtacgactgt acgttcttga actgtactgc

      961 atcatactgg gcctgcctcc ttgtctcaac attctgagaa ggaagcagcc acagctgaca

     1021 ttctttacca tcgctcttca gtcttgtcat taccagcgac tgcccccaca cattctctgg

     1081 gccaccgggt tgaaaagcgg cagcgagact cccgggacct cagagtccgc cacacccgaa

     1141 agtgataaaa agtattctat tggtttagcc atcggcacta attccgttgg atgggctgtc

     1201 ataaccgatg aatacaaagt accttcaaag aaatttaagg tgttggggaa cacagaccgt

     1261 cattcgatta aaaagaatct tatcggtgcc ctcctattcg atagtggcga aacggcagag

     1321 gcgactcgcc tgaaacgaac cgctcggaga aggtatacac gtcgcaagaa ccgaatatgt

     1381 tacttacaag aaatttttag caatgagatg gccaaagttg acgattcttt ctttcaccgt

     1441 ttggaagagt ccttccttgt cgaagaggac aagaaacatg aacggcaccc catctttgga

     1501 aacatagtag atgaggtggc atatcatgaa aagtacccaa cgatttatca cctcagaaaa

     1561 aagctagttg actcaactga taaagcggac ctgaggttaa tctacttggc tcttgcccat

     1621 atgataaagt tccgtgggca ctttctcatt gagggtgatc taaatccgga caactcggat

     1681 gtcgacaaac tgttcatcca gttagtacaa acctataatc agttgtttga agagaaccct

     1741 ataaatgcaa gtggcgtgga tgcgaaggct attcttagcg cccgcctctc taaatcccga

     1801 cggctagaaa acctgatcgc acaattaccc ggagagaaga aaaatgggtt gttcggtaac

     1861 cttatagcgc tctcactagg cctgacacca aattttaagt cgaacttcga cttagctgaa

     1921 gatgccaaat tgcagcttag taaggacacg tacgatgacg atctcgacaa tctactggca

     1981 caaattggag atcagtatgc ggacttattt ttggctgcca aaaaccttag cgatgcaatc

     2041 ctcctatctg acatactgag agttaatact gagattacca aggcgccgtt atccgcttca

     2101 atgatcaaaa ggtacgatga acatcaccaa gacttgacac ttctcaaggc cctagtccgt

     2161 cagcaactgc ctgagaaata taaggaaata ttctttgatc agtcgaaaaa cgggtacgca

     2221 ggttatattg acggcggagc gagtcaagag gaattctaca agtttatcaa acccatatta

     2281 gagaagatgg atgggacgga agagttgctt gtaaaactca atcgcgaaga tctactgcga

     2341 aagcagcgga ctttcgacaa cggtagcatt ccacatcaaa tccacttagg cgaattgcat

     2401 gctatactta gaaggcagga ggatttttat ccgttcctca aagacaatcg tgaaaagatt

     2461 gagaaaatcc taacctttcg cataccttac tatgtgggac ccctggcccg agggaactct

     2521 cggttcgcat ggatgacaag aaagtccgaa gaaacgatta ctccatggaa ttttgaggaa

     2581 gttgtcgata aaggtgcgtc agctcaatcg ttcatcgaga ggatgaccaa ctttgacaag

     2641 aatttaccga acgaaaaagt attgcctaag cacagtttac tttacgagta tttcacagtg

     2701 tacaatgaac tcacgaaagt taagtatgtc actgagggca tgcgtaaacc cgcctttcta

     2761 agcggagaac agaagaaagc aatagtagat ctgttattca agaccaaccg caaagtgaca

     2821 gttaagcaat tgaaagagga ctactttaag aaaattgaat gcttcgattc tgtcgagatc

     2881 tccggggtag aagatcgatt taatgcgtca cttggtacgt atcatgacct cctaaagata

     2941 attaaagata aggacttcct ggataacgaa gagaatgaag atatcttaga agatatagtg

     3001 ttgactctta ccctctttga agatcgggaa atgattgagg aaagactaaa aacatacgct

     3061 cacctgttcg acgataaggt tatgaaacag ttaaagaggc gtcgctatac gggctgggga

     3121 cgattgtcgc ggaaacttat caacgggata agagacaagc aaagtggtaa aactattctc

     3181 gattttctaa agagcgacgg cttcgccaat aggaacttta tgcagctgat ccatgatgac

     3241 tctttaacct tcaaagagga tatacaaaag gcacaggttt ccggacaagg ggactcattg

     3301 cacgaacata ttgcgaatct tgctggttcg ccagccatca aaaagggcat actccagaca

     3361 gtcaaagtag tggatgagct agttaaggtc atgggacgtc acaaaccgga aaacattgta

     3421 atcgagatgg cacgcgaaaa tcaaacgact cagaaggggc aaaaaaacag tcgagagcgg

     3481 atgaagagaa tagaagaggg tattaaagaa ctgggcagcc agatcttaaa ggagcatcct

     3541 gtggaaaata cccaattgca gaacgagaaa ctttacctct attacctaca aaatggaagg

     3601 gacatgtatg ttgatcagga actggacata aaccgtttat ctgattacga cgtcgatcac

     3661 attgtacccc aatccttttt gaaggacgat tcaatcgaca ataaagtgct tacacgctcg

     3721 gataagaacc gagggaaaag tgacaatgtt ccaagcgagg aagtcgtaaa gaaaatgaag

     3781 aactattggc ggcagctcct aaatgcgaaa ctgataacgc aaagaaagtt cgataactta