• 规格：20μl质粒
• 复制子：ori
• 质粒分类：基因文库质粒;其它基因质粒;其它种源质粒
• 质粒大小：3023bp
• 原核抗性：卡那霉素Kan（50μg/ml）
• 克隆菌株：DH5α等大肠杆菌
• 质粒简介：           pE6c质粒可以为GateWay反应提供EYFP基因模板，还可以通过BamHI和NotI酶切位点连入目的基因，进而和EYFP荧光基因一起导入到最终的表达载体，modified pENTR vector for C-terminal EYFP tag fusion with your gene of interest.
• 质粒图谱1： png/1-1FR31G42b55.png

质粒序列： 点击查看序列

LOCUS       Exported                3023 bp ds-DNA    circular SYN 14-八月-2017

KEYWORDS    pE6c

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 3023)

  TITLE     Direct Submission

  JOURNAL   Exported 2017年8月14日 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..3023

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     terminator      103..189

                     /gene="Escherichia coli rrnB"

                     /note="rrnB T1 term"

                     /note="transcription terminator T1 from the E. coli rrnB 

                     gene"

     terminator      281..308

                     /note="rrnB T2 term"

                     /note="transcription terminator T2 from the E. coli rrnB 

                     gene"

     protein_bind    358..457

                     /gene="mutant version of attL"

                     /bound_moiety="LR Clonase(TM)"

                     /note="attL1"

                     /note="recombination site for the Gateway(R) LR reaction"

     misc_feature    488..505

                     /note="stuffer"

     CDS             518..1237

                     /codon_start=1

                     /product="enhanced YFP"

                     /note="EYFP"

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     protein_bind    complement(1252..1351)

                     /gene="mutant version of attL"

                     /bound_moiety="LR Clonase(TM)"

                     /note="attL2"

                     /note="recombination site for the Gateway(R) LR reaction"

     CDS             1474..2283

                     /codon_start=1

                     /gene="aph(3')-Ia"

                     /product="aminoglycoside phosphotransferase"

                     /note="KanR"

                     /note="confers resistance to kanamycin in bacteria or G418 

                     (Geneticin(R)) in eukaryotes"

                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP

                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA

                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD

                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD

                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"

     rep_origin      2373..2961

                     /direction=RIGHT

                     /note="ori"

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg

       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct

      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt

      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg

      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat

      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa

      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc

      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac

      481 tggatccggt acagatttcg gacatgcggc cgcacctatg gtgagcaagg gcgaggagct

      541 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt

      601 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat

      661 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg

      721 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc

      781 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa

      841 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg

      901 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag

      961 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat

     1021 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc

     1081 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct

     1141 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc

     1201 cgggatcact ctcggcatgg acgagctgta caagtaactc gagatatcta gacccagctt

     1261 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg

     1321 tcactatcag tcaaaataaa atcattattt gccatccagc tgcagctctg gcccgtgtct

     1381 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg

     1441 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg

     1501 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat

     1561 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag

     1621 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga

     1681 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct

     1741 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa

     1801 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgtccct gcgccggttg

     1861 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag

     1921 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat

     1981 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat

     2041 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta

     2101 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc

     2161 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat

     2221 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc

     2281 taatcagaat tggttaattg gttgtaacat tattcagatt gggccccgtt ccactgagcg

     2341 tcagacccgg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc

     2401 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag

     2461 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt

     2521 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac

     2581 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc

     2641 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt

     2701 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt

     2761 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc

     2821 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt

     2881 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca

     2941 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt

     3001 tgctggcctt ttgctcacat gtt

//