• 规格：20μl质粒
• 启动子：Tac promoter
• 复制子：pBBR1 ori
• 终止子：TTTTTT
• 质粒大小：5915bp
• 原核抗性：卡那霉素Kan
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：            pBBR1MCS2-Tac-EGFP是一个广宿主表达质粒，广泛用于革兰氏阴性菌的蛋白表达，Tac启动子驱动EGFP的表达。
• 质粒图谱1： png/1-1G02Q42455102.png

质粒序列： 点击查看序列

LOCUS       Exported                5915 bp ds-DNA     circular SYN 25-SEP-2017

DEFINITION  synthetic circular DNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 5915)

  AUTHORS   yin

  TITLE     Direct Submission

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..5915

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     rep_origin      1023..1792

                     /label=pBBR1 oriV

                     /note="replication origin of the broad-host-range plasmid 

                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 

                     Rep protein for replication"

     CDS             1793..2455

                     /codon_start=1

                     /product="replication protein for the broad-host-range 

                     plasmid pBBR1 from Bordetella bronchiseptica"

                     /label=pBBR1 Rep

                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH

                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV

                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE

                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"

     misc_feature    3257..3285

                     /label=tac promoter

                     /note="strong E. coli promoter; hybrid between the trp and 

                     lac UV5 promoters"

     CDS             3315..4031

                     /codon_start=1

                     /product="enhanced GFP"

                     /label=EGFP

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     CDS             complement(4664..5458)

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=Kan

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

ORIGIN

        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg

       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt

      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg

      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg

      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca

      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt

      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg

      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt

      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg

      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct

      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg

      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc

      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca

      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg

      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg

      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc

      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc

     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga

     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc

     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt

     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg

     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac

     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc

     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg

     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg

     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag

     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta

     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg

     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc

     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac

     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga

     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct

     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac

     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt

     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt

     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag

     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct

     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga

     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac

     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct

     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt

     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg

     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga

     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga

     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg

     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa

     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa

     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag

     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc

     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca

     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg

     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg

     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga

     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc

     3241 tagaactagt ggatccttga caattaatca tcggctcgta taatgtgtgg aatttcacac

     3301 aggaaacagt attcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc

     3361 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg

     3421 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg

     3481 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc

     3541 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg

     3601 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg

     3661 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca

     3721 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg

     3781 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca

     3841 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc

     3901 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc

     3961 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg

     4021 agctgtacaa gtaatttttg aattcgatat caagcttatc gataccgtcg acctcgaggg

     4081 ggggcccggt acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat

     4141 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac

     4201 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa

     4261 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat

     4321 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgcatgcat aaaaactgtt

     4381 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa

     4441 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg ggggtgggcg

     4501 aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg

     4561 attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg

     4621 ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag aactcgtcaa

     4681 gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga

     4741 agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt

     4801 cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat

     4861 tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt

     4921 cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt

     4981 cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc

     5041 gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca

     5101 ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct

     5161 gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca

     5221 cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca

     5281 gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg

     5341 acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga

     5401 atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc

     5461 gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc cctgcgccat cagatccttg

     5521 gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc

     5581 cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg

     5641 taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag

     5701 cccagtagct gacattcatc ccaggtggca cttttcgggg aaatgtgcgc gcccgcgttc

     5761 ctgctggcgc tgggcctgtt tctggcgctg gacttcccgc tgttccgtca gcagcttttc

     5821 gcccacggcc ttgatgatcg cggcggcctt ggcctgcata tcccgattca acggccccag

     5881 ggcgtccaga acgggcttca ggcgctcccg aaggt

//