• 规格：20μl质粒
• 启动子：TRE3GV promoter
• 复制子：pUC ori
• 终止子：WPRE
• 质粒分类：基因文库质粒；人源基因质粒；人源真核质粒
• 质粒大小：10447bp
• 原核抗性：Amp（100μg/ml）
• 筛选标记：ZsGreen1，Puro
• 克隆菌株：Stbl3
• 表达宿主：293T等哺乳细胞
• 诱导方式：无需诱导
• 5测序引物：IRES-F（GTATGGGATCTGATCTGGGG）
• 3测序引物：PGK-R（GCGCCTACCGGTGGATGTGG）
• 备注：本质粒由武汉淼灵生物科技有限公司分享至淼灵质粒平台。
• 质粒简介：             pLVX-IRE3G-IDH2-R140Q-ZsGreen1质粒是将IDH2-R140Q人源基因克隆进入pLVX-TRE3G-ZsGreen1载体而得。TRE3GV promoter启动目的基因表达，并受到四环素的调控作用。该质粒可作为过表达质粒直接转染哺乳细胞，也可包装成慢病毒侵染细胞。质粒本身带有ZsGreen1荧光蛋白和Puro筛选标记，可利用荧光显微镜观察转染效率，也可用嘌呤霉素筛选转染后的细胞得到稳转细胞系。
             基因NCBI信息：Homo sapiens isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2), transcript variant 1, mRNA；NCBI Reference Sequence: NM_002168.3
             质粒分享者信息：武汉淼灵生物科技有限公司成立于2014年6月，是专业从事生物技术服务及生化试剂研发、生产和销售的公司。我们致力于研发出质优价适的精品试剂，打破国内企业在低端恶性竞争、国外公司漫天要价的痛心局面，不断开拓国内外市场，成为中国生物公司里的海尔和华为，国外生物公司中的ATCC和addgene。     
• 质粒图谱1： png/1-1FR311421R53.png

质粒序列： 点击查看序列

LOCUS       Exported               10447 bp ds-DNA    circular SYN 10-七月-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-IRE3G-ZsGreen1-IDH2-R140Q
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   liutao
  TITLE     Direct Submission
  JOURNAL   Exported 2017年7月10日 from MiaoLingPlasmid
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /note="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     misc_feature    2028..2144
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
     promoter        2206..2555
                     /note="TRE3GV promoter"
                     /note="3rd-generation Tet-responsive promoter that can be
                     activated by binding of Tet-On(R) 3G, optimized for
                     retroviral and lentiviral vectors"
     CDS             2564..3259
                     /codon_start=1
                     /note="ZsGreen1"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     misc_feature    3275..3849
                     /note="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3863..5221
                     /note="IDH2-R140Q"
     promoter        5232..5731
                     /note="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5752..6351
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6369..6957
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7165..7798
                     /note="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8329..8914)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9085..9945)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9946..10050)
                     /gene="bla"
                     /note="AmpR promoter"
     polyA_signal    10098..10232
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac
     2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga
     2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat
     2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag
     2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc
     2521 ctggagcaat tccacaacac ttttgtctta tacttggatc accatggctc agtcaaagca
     2581 cggcctgacc aaggagatga ccatgaagta ccgcatggag ggctgcgtgg acggccacaa
     2641 gttcgtgatc accggcgagg gcatcggcta ccccttcaag ggcaagcagg ccatcaacct
     2701 gtgcgtggtg gagggcggcc ccttgccctt cgccgaggac atcttgtccg ccgccttcat
     2761 gtacggcaac cgcgtgttca ccgagtaccc ccaggacatc gtcgactact tcaagaactc
     2821 ctgccccgcc ggctacacct gggaccgctc cttcctgttc gaggacggcg ccgtgtgcat
     2881 ctgcaacgcc gacatcaccg tgagcgtgga ggagaactgc atgtaccacg agtccaagtt
     2941 ctacggcgtg aacttccccg ccgacggccc cgtgatgaag aagatgaccg acaactggga
     3001 gccctcctgc gagaagatca tccccgtgcc caagcagggc atcttgaagg gcgacgtgag
     3061 catgtacctg ctgctgaagg acggtggccg cttgcgctgc cagttcgaca ccgtgtacaa
     3121 ggccaagtcc gtgccccgca agatgcccga ctggcacttc atccagcaca agctgacccg
     3181 cgaggaccgc agcgacgcca agaaccagaa gtggcacctg accgagcacg ccatcgcctc
     3241 cggatctgca ttgccctgag gccgccccgg ccggcccctc tccctccccc cccccctaac
     3301 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3361 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3421 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3481 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     3541 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     3601 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     3661 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     3721 ccccattgta tgggatctga tctggggcct cggtacacat gctttacatg tgtttagtcg
     3781 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     3841 cgatgataat atggcccccg ggatggccgg ctacctgcgg gtcgtgcgct cgctctgcag
     3901 agcctcaggc tcgcggccgg cctgggcgcc ggcggccctg acagccccca cctcgcaaga
     3961 gcagccgcgg cgccactatg ccgacaaaag gatcaaggtg gcgaagcccg tggtggagat
     4021 ggatggtgat gagatgaccc gtattatctg gcagttcatc aaggagaagc tcatcctgcc
     4081 ccacgtggac atccagctaa agtattttga cctcgggctc ccaaaccgtg accagactga
     4141 tgaccaggtc accattgact ctgcactggc cacccagaag tacagtgtgg ctgtcaagtg
     4201 tgccaccatc acccctgatg aggcccgtgt ggaagagttc aagctgaaga agatgtggaa
     4261 aagtcccaat ggaactatcc ggaacatcct gggggggact gtcttccggg agcccatcat
     4321 ctgcaaaaac atcccacgcc tagtccctgg ctggaccaag cccatcacca ttggcaagca
     4381 cgcccatggc gaccagtaca aggccacaga ctttgtggca gaccgggccg gcactttcaa
     4441 aatggtcttc accccaaaag atggcagtgg tgtcaaggag tgggaagtgt acaacttccc
     4501 cgcaggcggc gtgggcatgg gcatgtacaa caccgacgag tccatctcag gttttgcgca
     4561 cagctgcttc cagtatgcca tccagaagaa atggccgctg tacatgagca ccaagaacac
     4621 catactgaaa gcctacgatg ggcgtttcaa ggacatcttc caggagatct ttgacaagca
     4681 ctataagacc gacttcgaca agaataagat ctggtatgag caccggctca ttgatgacat
     4741 ggtggctcag gtcctcaagt cttcgggtgg ctttgtgtgg gcctgcaaga actatgacgg
     4801 agatgtgcag tcagacatcc tggcccaggg ctttggctcc cttggcctga tgacgtccgt
     4861 cctggtctgc cctgatggga agacgattga ggctgaggcc gctcatggga ccgtcacccg
     4921 ccactatcgg gagcaccaga agggccggcc caccagcacc aaccccatcg ccagcatctt
     4981 tgcctggaca cgtggcctgg agcaccgggg gaagctggat gggaaccaag acctcatcag
     5041 gtttgcccag atgctggaga aggtgtgcgt ggagacggtg gagagtggag ccatgaccaa
     5101 ggacctggcg ggctgcattc acggcctcag caatgtgaag ctgaacgagc acttcctgaa
     5161 caccacggac ttcctcgaca ccatcaagag caacctggac agagccctgg gcaggcagta
     5221 ggaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     5281 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     5341 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     5401 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     5461 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     5521 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     5581 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     5641 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     5701 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag
     5761 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     5821 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     5881 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     5941 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     6001 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     6061 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     6121 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     6181 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     6241 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     6301 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acggggcgcg
     6361 tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6421 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6481 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6541 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6601 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6661 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6721 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     6781 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6841 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6901 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     6961 gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     7021 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggtttttc
     7081 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7141 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata
     7201 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     7261 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     7321 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     7381 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     7441 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     7501 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     7561 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     7621 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7681 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7741 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7801 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     7861 agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga gcttggcgta
     7921 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7981 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8041 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8101 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8161 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8221 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8281 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8341 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8401 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8461 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8521 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8581 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8641 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8701 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8761 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8821 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa
     8881 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8941 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9001 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9061 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9121 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9181 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9241 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9301 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9361 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9421 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9481 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9541 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9601 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9661 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9721 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9781 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9841 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9901 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9961 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10021 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10081 cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    10141 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    10201 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    10261 atcccaaact tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta
    10321 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
    10381 tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt actacaaact
    10441 tagtagt
//