- 商品货号:FH1314
- 规格:10μl质粒
- 启动子:minimal CMV promoter
- 复制子:pUC ori,F1 ori,SV40 ori
- 终止子:WPRE
- 质粒分类:基因文库质粒;人源基因质粒;人源真核质粒
- 质粒大小:9471bp
- 原核抗性:Amp(100μg/ml)
- 筛选标记:Zeo
- 克隆菌株:Stbl3
- 表达宿主:293T等哺乳细胞
- 诱导方式:四环素调控
- 5测序引物:miniCMV-F(GCGTGTACGGTGGGAGGCC)
- 3测序引物:WPRE-R(CATAGCGTAAAAGGAGCAACA)
- 质粒简介: pFU-tet-o-hOct4质粒是一个人源Oct4基因的哺乳细胞慢病毒过表达质粒,minimal CMV promoter启动Oct4目的基因的表达。该质粒可作为过表达质粒直接转染哺乳细胞,也可包装成慢病毒侵染细胞。质粒本身带有Zeo筛选标记,可用博来霉素筛选转染后的细胞得到稳转细胞系。基因NCBI信息:Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1, mRNA;NCBI Reference Sequence: NM_002701.5.
- 质粒图谱1: png/1-1FG10T033Y5.png
质粒序列: 点击查看序列LOCUS Exported 9471 bp ds-DNA circular SYN 11-七月-2017
KEYWORDS pFU-tet-o-hOct4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9471)
TITLE Direct Submission
JOURNAL Exported 2017年7月11日 from MiaoLingPlasmid
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9471
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 238..617
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 835..1015
/note="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/note="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/note="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2440..2557
/note="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2610..2880
/bound_moiety="tetracycline repressor TetR"
/note="tetracycline response element"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 2913..2950
/note="minimal CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 3056..4138
/codon_start=1
/product="Homo sapiens POU class 5 homeobox 1 (POU5F1),
transcript variant 1, mRNA
NCBI Reference Sequence: NM_002701.5"
/note="Oct4"
/translation="MAGHLASDFAFSPPPGGGGDGPGGPEPGWVDPRTWLSFQGPPGGP
GIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLVPQGGLETSQPEGEAGVGVE
SNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFAKLLKQKRITLGYT
QADVGLTLGVLFGKVFSQTTICRFEALQLSFKNMCKLRPLLQKWVEEADNNENLQEICK
AETLVQARKRKRTSIENRVRGNLENLFLQCPKPTLQQISHIAQQLGLEKDVVRVWFCNR
RQKGKRSSSDYAQREDFEAAGSPFSGGPVSFPLAPGPHFGTPGYGSPHFTALYSSVPFP
EGEAFPPVSVTTLGSPMHSN"
misc_feature 4163..4751
/note="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 5276..5456
/note="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
polyA_signal 5488..5712
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 5758..6186
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6200..6529
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 6380..6515
/note="SV40 ori"
/note="SV40 origin of replication"
promoter 6577..6624
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 6643..7017
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="Zeo"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 7147..7268
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(7719..8307)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8478..9338)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
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