• 规格：20μl质粒
• 启动子：minimal CMV promoter
• 复制子：pUC ori,F1 ori,SV40 ori
• 终止子：WPRE
• 质粒分类：基因文库质粒；人源基因质粒；人源真核质粒
• 质粒大小：9471bp
• 原核抗性：Amp（100μg/ml）
• 筛选标记：Zeo
• 克隆菌株：Stbl3
• 表达宿主：293T等哺乳细胞
• 诱导方式：四环素调控
• 5测序引物：miniCMV-F（GCGTGTACGGTGGGAGGCC）
• 3测序引物：WPRE-R（CATAGCGTAAAAGGAGCAACA）
• 质粒简介：              pFU-tet-o-hOct4质粒是一个人源Oct4基因的哺乳细胞慢病毒过表达质粒，minimal CMV promoter启动Oct4目的基因的表达。该质粒可作为过表达质粒直接转染哺乳细胞，也可包装成慢病毒侵染细胞。质粒本身带有Zeo筛选标记，可用博来霉素筛选转染后的细胞得到稳转细胞系。基因NCBI信息：Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1, mRNA；NCBI Reference Sequence: NM_002701.5.
  
• 质粒图谱1： png/1-1FG10T033Y5.png

质粒序列： 点击查看序列

LOCUS       Exported                9471 bp ds-DNA    circular SYN 11-七月-2017
KEYWORDS    pFU-tet-o-hOct4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9471)
 TITLE     Direct Submission
  JOURNAL   Exported 2017年7月11日 from MiaoLingPlasmid
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9471
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        238..617
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     misc_feature    2440..2557
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    2610..2880
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        2913..2950
                     /note="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             3056..4138
                     /codon_start=1
                     /product="Homo sapiens POU class 5 homeobox 1 (POU5F1),
                     transcript variant 1, mRNA
                     NCBI Reference Sequence: NM_002701.5"
                     /note="Oct4"
                     /translation="MAGHLASDFAFSPPPGGGGDGPGGPEPGWVDPRTWLSFQGPPGGP
                     GIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLVPQGGLETSQPEGEAGVGVE
                     SNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFAKLLKQKRITLGYT
                     QADVGLTLGVLFGKVFSQTTICRFEALQLSFKNMCKLRPLLQKWVEEADNNENLQEICK
                     AETLVQARKRKRTSIENRVRGNLENLFLQCPKPTLQQISHIAQQLGLEKDVVRVWFCNR
                     RQKGKRSSSDYAQREDFEAAGSPFSGGPVSFPLAPGPHFGTPGYGSPHFTALYSSVPFP
                     EGEAFPPVSVTTLGSPMHSN"
     misc_feature    4163..4751
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5276..5456
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5488..5712
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5758..6186
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6200..6529
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      6380..6515
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        6577..6624
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             6643..7017
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="Zeo"
                     /note="confers resistance to bleomycin, phleomycin, and
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7147..7268
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(7719..8307)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8478..9338)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW