• 规格：20μl质粒
• 启动子：MSCV LTR promoter
• 复制子：ori
• 终止子：3
• 质粒分类：基因文库质粒；其它基因质粒；其它种源质粒
• 质粒大小：7057bp
• 质粒标签：N-FLAG，N-HA
• 原核抗性：氨苄青霉素Amp（100μg/ml）
• 筛选标记：EGFP
• 克隆菌株：Stbl3等大肠杆菌
• 表达宿主：293T等哺乳细胞
• 诱导方式：无需诱导
• 质粒简介：           Retroviral vector for Flag-HA-tagged HPV16 E6 expression. No starting ATG in viral ORF, translation begins from tag only. Puro resistance protein expressed from an IRES.
• 质粒图谱1： png/1-1FQ4164K52S.png

质粒序列： 点击查看序列

LOCUS       Exported                7057 bp ds-DNA     circular SYN 14-AUG-2017

DEFINITION  synthetic circular DNA

KEYWORDS    p6659 MSCV-IP N FlagHA HPV16E6

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 7057)

   TITLE     Direct Submission

  JOURNAL   Exported Aug 14, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..7057

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    579..920

                     /label=MESV Psi

                     /note="packaging signal of murine embryonic stem cell 

                     virus"

     CDS             987..1403

                     /label=gag (truncated)

                     /note="truncated Moloney murine leukemia virus (MMLV) gag 

                     gene lacking the start codon"

     regulatory      1416..1425

                     /regulatory_class="other"

                     /note="vertebrate consensus sequence for strong initiation 

                     of translation (Kozak, 1987)"

     CDS             1425..1448

                     /codon_start=1

                     /product="FLAG(R) epitope tag, followed by an enterokinase 

                     cleavage site"

                     /label=FLAG

                     /translation="DYKDDDDK"

     CDS             1461..1487

                     /codon_start=1

                     /product="HA (human influenza hemagglutinin) epitope tag"

                     /label=HA

                     /translation="YPYDVPDYA"

     protein_bind    1503..1527

                     /gene="mutant version of attB"

                     /label=attB1

                     /bound_moiety="BP Clonase(TM)"

                     /note="recombination site for the Gateway(R) BP reaction"

     misc_feature    1528..1979

                     /label=HPV16E6

     protein_bind    complement(1981..2005)

                     /gene="mutant version of attB"

                     /label=attB2

                     /bound_moiety="BP Clonase(TM)"

                     /note="recombination site for the Gateway(R) BP reaction"

     misc_feature    2035..2586

                     /label=IRES

                     /note="internal ribosome entry site (IRES) of the 

                     encephalomyocarditis virus (EMCV)"

     CDS             2598..3317

                     /codon_start=1

                     /product="enhanced GFP"

                     /label=EGFP

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     LTR             3419..3933

                     /label=3' LTR

                     /note="3' long terminal repeat from murine embryonic stem 

                     cell virus"

     rep_origin      complement(4504..5092)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(5263..6123)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(6124..6228)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat

       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca

      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga

      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt

      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc

      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc

      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata

      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca

      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga

      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg

      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt

      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa

      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga

      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag

      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa

      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg

      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct

     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga

     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag

     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc

     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc

     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg

     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag

     1381 ccctcactcc ttctctaggc gccggaatta gatctgccac catggactac aaggatgacg

     1441 atgacaagct cgatggagga tacccatacg atgttccaga ttacgctgct cgaggttatc

     1501 aaacaagttt gtacaaaaaa gttggctttc aggacccaca ggagcgaccc agaaagttac

     1561 cacagttatg cacagagctg caaacaacta tacatgatat aatattagaa tgtgtgtact

     1621 gcaagcaaca gttactgcga cgtgaggtat atgactttgc ttttcgggat ttatgtatag

     1681 tatatagaga tgggaatcca tatgctgtat gtgataaatg tttaaagttt tattctaaaa

     1741 ttagtgagta tagacattat tgttatagtg tgtatggaac aacattagaa cagcaataca

     1801 acaaaccgtt gtgtgatttg ttaattaggt gtattaactg tcaaaagcca ctgtgtcctg

     1861 aagaaaagca aagacatctg gacaaaaagc aaagattcca taatataagg ggtcggtgga

     1921 ccggtcgatg tatgtcttgt tgcagatcat caagaacacg tagagaaacc cagctgtaat

     1981 acccaacttt cttgtacaaa gtggttcgat aacgaattcc gccccccccc cctaacgtta

     2041 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca

     2101 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca

     2161 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg

     2221 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc

     2281 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata

     2341 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag

     2401 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc

     2461 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt

     2521 taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg

     2581 ataatatggc cacaaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca

     2641 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg

     2701 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc

     2761 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct

     2821 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc

     2881 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt

     2941 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg

     3001 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg

     3061 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg

     3121 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc

     3181 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga

     3241 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg

     3301 acgagctgta caagtaatga attaattaag aattatcaag cttatcgata ccgtcgacct

     3361 gcagccaagc ttatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat

     3421 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg

     3481 gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag

     3541 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa

     3601 cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat cagatgtttc

     3661 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg

     3721 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc

     3781 tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa

     3841 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga

     3901 gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa

     3961 caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt

     4021 gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag aagag