• 商品货号：FH1383

• 规格：10μl质粒
• 启动子：hCMV IE4 promoter
• 复制子：pUC ori
• 终止子：SV40 poly(A) signal
• 质粒分类：基因文库质粒；其它基因质粒；其它种源质粒
• 质粒大小：7010bp
• 原核抗性：氨苄青霉素Amp（100μg/ml）
• 筛选标记：新霉素Neo/G418
• 克隆菌株：DH5α等大肠杆菌
• 表达宿主：293T等哺乳细胞
• 诱导方式：无需诱导
• 质粒简介：       GW1-PercevalHR质粒通过hCMV IE4 启动子驱动PercevalHR基因的表达，Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.
• 质粒图谱1： png/1-1FQ4160JLR.png

质粒序列： 点击查看序列

LOCUS       Exported                7010 bp ds-DNA    circular SYN 14-八月-2017

KEYWORDS    GW1-PercevalHR

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 7010)

  TITLE     Direct Submission

  JOURNAL   Exported 2017年8月14日 from MiaoLingPlasmid

            http://www.snapgene.com

FEATURES             Location/Qualifiers

     source          1..7010

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             189..1928

                     /codon_start=1

                     /note="PercevalHR"

                     /translation="MKKVESIIRPEKLEIVKKALSDAGYVGMTVSEVKGSGVQGGIFER

                     YRGRVYSAGYNSDNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVL

                     LPDNHYLSFQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFT

                     GVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGL

                     QCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKG

                     IDFKEDGNILGHKLEYNGTIVDLIPKVKIELVVKEEDVDNVIDIICENARTGNPGDGKI

                     FVIPVERVVRVRTKEEGASGGGSGGGGASGMKKVEAIIRPEKLEIVKKALSDAGYVGMT

                     VSEVKGRGAGGGDLIPKVKIELVVKEEDVDNVIDIICENARTGNPGDGKIFVIPVERIV

                     RVRTKEEGASGGGGGSGGASGMKKVEAIIRPEKLEIVKKALNDDGYVGMTVSEVKGRGA

                     GGGDLIPKVKIELVVKEEDVDNIIDIICENARTGNPGDGKIFVIPVERVVRPRTKEEGK

                     EAL"

     polyA_signal    2050..2184

                     /note="SV40 poly(A) signal"

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(2610..3198)

                     /direction=LEFT

                     /note="ori"

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(3369..4229)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /note="AmpR"

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     misc_feature    5448..7010

                     /note="hCMV IE4 promoter"

     enhancer        5448..5827

                     /note="CMV enhancer"

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        5828..6031

                     /note="CMV promoter"

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     misc_feature    6032..7010

                     /note="CMV intron"

ORIGIN

        1 aagcttcccg gccacagcaa tcgatccatg acacaaccag tgacaagcag tcccccagtg

       61 aaaccgcatc cgcatccgag tccgataccg ataaagattc tgaatcggag tgagtgccgc

      121 gtccgagagc gttccctgtc cacgtccacc atcggcggag caggtgtgcc tgaggcctct

      181 agaccaccat gaaaaaggtt gaatccatca tcaggcccga aaagctggag atcgttaaga

      241 aggctctctc ggacgctgga tatgtgggta tgaccgtctc tgaagtcaaa ggctctggcg

      301 tccagggcgg catcttcgag aggtaccgag gaagggtgta ctctgcaggc tacaacagcg

      361 acaacgtcta catcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc

      421 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca

      481 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcttccag tccaagctga

      541 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg

      601 ggatcactct cggcatggac gagctgtaca agggcggttc cggaggcatg gtgagcaagg

      661 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg

      721 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc

      781 tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc

      841 tgggctacgg tctccaatgc ttcgcccgct accccgacca catgaagcag cacgacttct

      901 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg

      961 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg

     1021 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag cttgagtaca

     1081 acggcaccat agtagatctg atccctaagg taaaaattga actagtggtg aaggaggagg

     1141 acgtcgataa cgtgatagac attatttgcg aaaatgcccg cacaggtaac cccggcgacg

     1201 gtaagatatt tgtgatccca gtggagcgag tggtcagggt gcgaaccaaa gaggagggag

     1261 catctggtgg tggatccggt ggtggcggtg catctggtat gaaaaaggtt gaagctatta

     1321 ttcgcccaga gaaactggag attgttaaga aggccctgtc cgacgccggc tatgttggca

     1381 tgaccgtttc cgaggttaaa ggccgcggtg ccggcggtgg cgacctgatt cctaaggtga

     1441 agatcgagct ggttgttaag gaggaagacg ttgataatgt tatcgacatt atctgcgaga

     1501 acgcccgcac tggcaatcct ggtgacggca aaatcttcgt tataccggtc gagcgaattg

     1561 tgcgagtacg aaccaaagaa gagggtgcct ccggcggtgg cggtggctcc ggtggcgcca

     1621 gcggcatgaa gaaggttgag gccatcatcc gcccagagaa gctggaaatc gttaagaagg

     1681 ccttaaacga cgacggctac gtcggcatga cagttagcga ggttaaaggt cgcggtgccg

     1741 gtgggggtga tctgattcct aaggttaaaa tcgagctcgt tgttaaggag gaggacgttg

     1801 acaacattat cgatatcatc tgcgaaaacg ctcgcaccgg caacccaggc gacggcaaga

     1861 ttttcgtcat tcctgttgag agagtggtgc gtccccgtac gaaggaggag ggcaaggaag

     1921 cactgtgact cgacgaattc attgatcata atcagccata ccacatttgt agaggtttta

     1981 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt

     2041 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca

     2101 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc

     2161 aatgtatctt atcatgtctg gatccgtacc gagctcgcgt aatcatggtc atagctgttt

     2221 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag

     2281 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg

     2341 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg

     2401 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc

     2461 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc

     2521 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg

     2581 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat

     2641 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag

     2701 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga

     2761 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg

     2821 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt

     2881 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac

     2941 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc

     3001 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt

     3061 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc

     3121 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc

     3181 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg

     3241 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag

     3301 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg

     3361 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt

     3421 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca

     3481 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca

     3541 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc

     3601 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt

     3661 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg

     3721 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc

     3781 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg

     3841 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga

     3901 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga

     3961 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta

     4021 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg

     4081 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact

     4141 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata

     4201 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaataagc ggccgcggcc

     4261 atgccggcca ctagtctcga gttattattg aagcatttat cagggttatt gtctcatgag

     4321 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc

     4381 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa

     4441 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg

     4501 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca

     4561 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc

     4621 atcagagcag attgtactga gagtgcacca tatgaagacg tcgcctcctc actacttctg

     4681 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcg

     4741 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg

     4801 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca

     4861 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccatctgcag tgaataataa

     4921 aatgtgtgtt tgtccgaaat acgcgtttga gatttctgtc ccgactaaat tcatgtcgcg

     4981 cgatagtggt gtttatcgcc gatagagatg gcgatattgg aaaaatcgat atttgaaaat

     5041 atggcatatt gaaaatgtcg ccgatgtgag tttctgtgta actgatatcg ccatttttcc

     5101 aaaagttgat ttttgggcat acgcgatatc tggcgatacg cttatatcgt ttacggggga

     5161 tggcgataga cgcctttggt gacttgggcg attctgtgtg tcgcaaatat cgcagtttcg

     5221 atataggtga cagacgatat gaggctatat cgccgataga ggcgacatca agctggcaca

     5281 tggccaatgc atatcgatct atacattgaa tcaatattgg ccattagcca tattattcat

     5341 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atccatatca

     5401 taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac attgattatt

     5461 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt

     5521 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc

     5581 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg

     5641 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat

     5701 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca

     5761 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat