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- 规格：20μl质粒
- 启动子：hCMV IE4 promoter
- 复制子：pUC ori
- 终止子：SV40 poly(A) signal
- 质粒分类：基因文库质粒；其它基因质粒；其它种源质粒
- 质粒大小：7010bp
- 原核抗性：氨苄青霉素Amp（100μg/ml）
- 筛选标记：新霉素Neo/G418
- 克隆菌株：DH5α等大肠杆菌
- 表达宿主：293T等哺乳细胞
- 诱导方式：无需诱导
- 质粒简介： GW1-PercevalHR质粒通过hCMV IE4 启动子驱动PercevalHR基因的表达，Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.
- 质粒图谱1： png/1-1FQ4160JLR.png
质粒序列： 点击查看序列LOCUS Exported 7010 bp ds-DNA circular SYN 14-八月-2017KEYWORDS GW1-PercevalHRSOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 7010)TITLE Direct SubmissionJOURNAL Exported 2017年8月14日 from MiaoLingPlasmidhttp://www.snapgene.comFEATURES Location/Qualifierssource 1..7010/organism="synthetic DNA construct"/mol_type="other DNA"CDS 189..1928/codon_start=1/note="PercevalHR"/translation="MKKVESIIRPEKLEIVKKALSDAGYVGMTVSEVKGSGVQGGIFERYRGRVYSAGYNSDNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSFQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNGTIVDLIPKVKIELVVKEEDVDNVIDIICENARTGNPGDGKIFVIPVERVVRVRTKEEGASGGGSGGGGASGMKKVEAIIRPEKLEIVKKALSDAGYVGMTVSEVKGRGAGGGDLIPKVKIELVVKEEDVDNVIDIICENARTGNPGDGKIFVIPVERIVRVRTKEEGASGGGGGSGGASGMKKVEAIIRPEKLEIVKKALNDDGYVGMTVSEVKGRGAGGGDLIPKVKIELVVKEEDVDNIIDIICENARTGNPGDGKIFVIPVERVVRPRTKEEGKEAL"polyA_signal 2050..2184/note="SV40 poly(A) signal"/note="SV40 polyadenylation signal"rep_origin complement(2610..3198)/direction=LEFT/note="ori"/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(3369..4229)/codon_start=1/gene="bla"/product="beta-lactamase"/note="AmpR"/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"misc_feature 5448..7010/note="hCMV IE4 promoter"enhancer 5448..5827/note="CMV enhancer"/note="human cytomegalovirus immediate early enhancer"promoter 5828..6031/note="CMV promoter"/note="human cytomegalovirus (CMV) immediate earlypromoter"misc_feature 6032..7010/note="CMV intron"ORIGIN1 aagcttcccg gccacagcaa tcgatccatg acacaaccag tgacaagcag tcccccagtg61 aaaccgcatc cgcatccgag tccgataccg ataaagattc tgaatcggag tgagtgccgc121 gtccgagagc gttccctgtc cacgtccacc atcggcggag caggtgtgcc tgaggcctct181 agaccaccat gaaaaaggtt gaatccatca tcaggcccga aaagctggag atcgttaaga241 aggctctctc ggacgctgga tatgtgggta tgaccgtctc tgaagtcaaa ggctctggcg301 tccagggcgg catcttcgag aggtaccgag gaagggtgta ctctgcaggc tacaacagcg361 acaacgtcta catcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc421 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca481 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcttccag tccaagctga541 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg601 ggatcactct cggcatggac gagctgtaca agggcggttc cggaggcatg gtgagcaagg661 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg721 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc781 tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc841 tgggctacgg tctccaatgc ttcgcccgct accccgacca catgaagcag cacgacttct901 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg961 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg1021 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag cttgagtaca1081 acggcaccat agtagatctg atccctaagg taaaaattga actagtggtg aaggaggagg1141 acgtcgataa cgtgatagac attatttgcg aaaatgcccg cacaggtaac cccggcgacg1201 gtaagatatt tgtgatccca gtggagcgag tggtcagggt gcgaaccaaa gaggagggag1261 catctggtgg tggatccggt ggtggcggtg catctggtat gaaaaaggtt gaagctatta1321 ttcgcccaga gaaactggag attgttaaga aggccctgtc cgacgccggc tatgttggca1381 tgaccgtttc cgaggttaaa ggccgcggtg ccggcggtgg cgacctgatt cctaaggtga1441 agatcgagct ggttgttaag gaggaagacg ttgataatgt tatcgacatt atctgcgaga1501 acgcccgcac tggcaatcct ggtgacggca aaatcttcgt tataccggtc gagcgaattg1561 tgcgagtacg aaccaaagaa gagggtgcct ccggcggtgg cggtggctcc ggtggcgcca1621 gcggcatgaa gaaggttgag gccatcatcc gcccagagaa gctggaaatc gttaagaagg1681 ccttaaacga cgacggctac gtcggcatga cagttagcga ggttaaaggt cgcggtgccg1741 gtgggggtga tctgattcct aaggttaaaa tcgagctcgt tgttaaggag gaggacgttg1801 acaacattat cgatatcatc tgcgaaaacg ctcgcaccgg caacccaggc gacggcaaga1861 ttttcgtcat tcctgttgag agagtggtgc gtccccgtac gaaggaggag ggcaaggaag1921 cactgtgact cgacgaattc attgatcata atcagccata ccacatttgt agaggtttta1981 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt2041 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca2101 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc2161 aatgtatctt atcatgtctg gatccgtacc gagctcgcgt aatcatggtc atagctgttt2221 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag2281 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg2341 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg2401 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc2461 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc2521 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg2581 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat2641 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag2701 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga2761 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg2821 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt2881 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac2941 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc3001 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt3061 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc3121 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc3181 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg3241 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag3301 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg3361 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt3421 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca3481 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca3541 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc3601 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt3661 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg3721 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc3781 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg3841 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga3901 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga3961 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta4021 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg4081 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact4141 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata4201 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaataagc ggccgcggcc4261 atgccggcca ctagtctcga gttattattg aagcatttat cagggttatt gtctcatgag4321 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc4381 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa4441 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg4501 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca4561 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc4621 atcagagcag attgtactga gagtgcacca tatgaagacg tcgcctcctc actacttctg4681 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcg4741 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg4801 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca4861 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccatctgcag tgaataataa4921 aatgtgtgtt tgtccgaaat acgcgtttga gatttctgtc ccgactaaat tcatgtcgcg4981 cgatagtggt gtttatcgcc gatagagatg gcgatattgg aaaaatcgat atttgaaaat5041 atggcatatt gaaaatgtcg ccgatgtgag tttctgtgta actgatatcg ccatttttcc5101 aaaagttgat ttttgggcat acgcgatatc tggcgatacg cttatatcgt ttacggggga5161 tggcgataga cgcctttggt gacttgggcg attctgtgtg tcgcaaatat cgcagtttcg5221 atataggtga cagacgatat gaggctatat cgccgataga ggcgacatca agctggcaca5281 tggccaatgc atatcgatct atacattgaa tcaatattgg ccattagcca tattattcat5341 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atccatatca5401 taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac attgattatt5461 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt5521 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc5581 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg5641 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat5701 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca5761 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
