• 商品货号：FH1384

• 规格：10μl质粒
• 启动子：T7 promoter
• 复制子：Ori，F1 ori
• 终止子：rrnB T1 terminator
• 质粒分类：大肠杆菌质粒；大肠其它质粒；其它大肠质粒
• 质粒大小：6495bp
• 质粒标签：SNAP-tag
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：             pSNAP-Tag(T7)2是一个大肠杆菌表达质粒，T7启动子驱动SNAP标签和目的基因融合表达。pSNAP-tag (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag® protein. Expression is under control of the IPTG inducible T7 promoter. The pSNAP-tag(T7)-2 Vector can also be used as a control plasmid expressing the SNAP-tag protein (20 kDa). The target gene should be cloned as a fusion to the Nor C-terminus of the SNAP-tag. The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6 - alkylguanine-DNA-alkyltransferase (hAGT)(1,2,3). SNAP-tag substrates are derivatives of benzyl purines and benzyl pyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Cloning and expression of SNAP-tag fusion proteins are described in this document. The labeling of the fusion proteins with SNAP-tag substrates is described in the instructions supplied with SNAPtag substrates.
• 质粒图谱1： png/1-1FQG5215c41.png

质粒序列： 点击查看序列

LOCUS       Exported                6495 bp ds-DNA    circular SYN 17-七月-2017

KEYWORDS    pSNAP-tag(T7)2

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 6495)

  AUTHORS   MiaoLingBio

FEATURES             Location/Qualifiers

     source          1..6495

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        35..139

                     /gene="bla"

                     /note="AmpR promoter"

     CDS             140..1000

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /note="AmpR"

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      complement(1100..1555)

                     /direction=LEFT

                     /note="F1 ori"

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     rep_origin      1666..2254

                     /direction=RIGHT

                     /note="ori"

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(2623..2814)

                     /codon_start=1

                     /gene="rop"

                     /product="Rop protein, which maintains plasmids at low copy

                     number"

                     /note="rop"

                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA

                     DELYRSCLARFGDDGENL"

     CDS             complement(3371..4453)

                     /codon_start=1

                     /gene="lacI"

                     /product="lac repressor"

                     /note="lacI"

                     /note="The lac repressor binds to the lac operator to 

                     inhibit transcription in E. coli. This inhibition can be 

                     relieved by adding lactose or 

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL

                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV

                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH

                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA

                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC

                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR

                     ALADSLMQLARQVSRLESGQ"

     promoter        complement(4454..4531)

                     /gene="lacI"

                     /note="lacI promoter"

                     /note="

                     "

     terminator      4684..4770

                     /gene="Escherichia coli rrnB"

                     /note="rrnB T1 terminator"

                     /note="transcription terminator T1 from the E. coli rrnB 

                     gene"

     terminator      4867..4953

                     /gene="Escherichia coli rrnB"

                     /note="rrnB T1 terminator"

                     /note="transcription terminator T1 from the E. coli rrnB 

                     gene"

     terminator      5050..5136

                     /gene="Escherichia coli rrnB"

                     /note="rrnB T1 terminator"

                     /note="transcription terminator T1 from the E. coli rrnB 

                     gene"

     terminator      5233..5319

                     /gene="Escherichia coli rrnB"

                     /note="rrnB T1 terminator"

                     /note="transcription terminator T1 from the E. coli rrnB 

                     gene"

     terminator      5416..5502

                     /gene="Escherichia coli rrnB"

                     /note="rrnB T1 terminator"

                     /note="transcription terminator T1 from the E. coli rrnB 

                     gene"

     promoter        5637..5655

                     /note="T7 promoter"

                     /note="promoter for bacteriophage T7 RNA polymerase"

     protein_bind    5656..5680

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="lac operator"

                     /note="The lac repressor binds to the lac operator to 

                     inhibit transcription in E. coli. This inhibition can be 

                     relieved by adding lactose or 

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     CDS             5767..6312

                     /codon_start=1

                     /product="human O6-alkylguanine-DNA-alkyltransferase 

                     variant that forms covalent bonds with benzylguanine 

                     derivatives"

                     /note="SNAP-tag(R)"

                     /note="optimized for bacterial expression"

                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE

                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK

                     VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA

                     VKEWLLAHEGHRLGKPGLG"

     terminator      6423..6470

                     /note="T7 terminator"

                     /note="transcription terminator for bacteriophage T7 RNA 

                     polymerase"

ORIGIN

        1 aactacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt

       61 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata

      121 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt

      181 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc

      241 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat

      301 ccttgagagt tttcgccccg aagaacgttt cccaatgatg agcactttta aagttctgct

      361 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca

      421 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg

      481 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa

      541 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg

      601 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga

      661 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg

      721 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt

      781 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg

      841 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc

      901 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca

      961 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc

     1021 atatatactt tagattgatt taccccggtt gataatcaga aaagccccaa aaacaggaag

     1081 attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat

     1141 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa

     1201 tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta

     1261 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca

     1321 ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat

     1381 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg

     1441 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc

     1501 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaaag

     1561 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc

     1621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt

     1681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt

     1741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat

     1801 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc

     1861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa

     1921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg

     1981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag

     2041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag