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- 规格：20μl质粒
- 启动子：T7 promoter
- 复制子：Ori，F1 ori
- 终止子：rrnB T1 terminator
- 质粒分类：大肠杆菌质粒；大肠其它质粒；其它大肠质粒
- 质粒大小：6495bp
- 质粒标签：SNAP-tag
- 原核抗性：氨苄青霉素Amp
- 克隆菌株：大肠杆菌DH5α
- 质粒简介： pSNAP-Tag(T7)2是一个大肠杆菌表达质粒，T7启动子驱动SNAP标签和目的基因融合表达。pSNAP-tag (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag® protein. Expression is under control of the IPTG inducible T7 promoter. The pSNAP-tag(T7)-2 Vector can also be used as a control plasmid expressing the SNAP-tag protein (20 kDa). The target gene should be cloned as a fusion to the Nor C-terminus of the SNAP-tag. The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6 - alkylguanine-DNA-alkyltransferase (hAGT)(1,2,3). SNAP-tag substrates are derivatives of benzyl purines and benzyl pyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Cloning and expression of SNAP-tag fusion proteins are described in this document. The labeling of the fusion proteins with SNAP-tag substrates is described in the instructions supplied with SNAPtag substrates.
- 质粒图谱1： png/1-1FQG5215c41.png
质粒序列： 点击查看序列
