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- 规格：20μl质粒
- 启动子：CMV IE promoter
- 复制子：pUC ori，F1 ori，SV40 ori
- 终止子：SV40 early polyA signals
- 质粒分类：哺乳细胞质粒；哺乳荧光质粒；哺乳红色质粒
- 质粒大小：4698bp
- 质粒标签：N-HcRed1
- 原核抗性：卡那霉素Kan（50μg/μl）
- 筛选标记：新霉素Neo/G418
- 克隆菌株：DH5α等大肠杆菌
- 表达宿主：293T等哺乳细胞
- 诱导方式：无需诱导，组成型表达
- 5测序引物：CMV-F：CGCAAATGGGCGGTAGGCGTG
- 3测序引物：Sv40-polyA-R：GAAATTTGTGATGCTATTGC
- 备注：高拷贝
- 质粒简介： pHcRed1-C1质粒是一个哺乳细胞红色荧光蛋白表达载体，CMV IE启动子驱动HcRed1红色荧光蛋白基因和目的基因融合表达。
pHcRed1-C1 is a mammalian expression vector designed to express a protein of interest fused to the C-terminus of the far-red fluorescent protein HcRed1. pHcRed1-C1 can be used to monitor gene expression and protein localization in vivo or as a cotransfection marker; the unmodified vector will express HcRed1 in mammalian cells. HcRed1 was generated by mutagenesis of a non-fluorescent chromoprotein from the reef coral Heteractis crispa . The coding sequence for HcRed1 is human codon-optimized for higher expression in mammalian cells . Genes cloned into the multiple cloning site (MCS) downstream of the HcRed1 coding sequence are expressed as fusions to the C-terminus of HcRed1. The MCS in pHcRed1-C1 is positioned between the HcRed1 coding sequence and the SV40 polyadenylation signal (SV40 poly A). Genes cloned into the MCS will be expressed as fusions to the C-terminus of HcRed1 if they are in the same reading frame as HcRed1 and there are no intervening stop codons. A Kozak consensus sequence upstream of HcRed1 increases the translation efficiency in eukaryotic cells . SV40 poly A signals downstream of the MCS direct proper processing of the 3' end of mRNA transcripts. The vector also contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen, a pUC origin of replication for propagation in E. coli, and an f1 origin for single-stranded DNA production. A neomycin resistance cassette (Neor)—consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene—allows stably transfected eukaryotic cells to be selected using G418. A bacterial promoter upstream of this cassette expresses kanamycin resistance in E. coli. pHcRed1-C1 can be transfected into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418 .- 质粒图谱1： png/1-1FR5115920238.png
- 质粒图谱2： png/1-1FR5115933241.png
质粒序列： 点击查看序列
