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- 规格：20μl质粒
- 质粒图谱1： png/1-1FR31436043Z.png
质粒序列： 点击查看序列LOCUS Exported 5992 bp ds-DNA circular SYN 26-SEP-2017DEFINITION synthetic circular DNAACCESSION .VERSION .KEYWORDSSOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 5992)AUTHORS .TITLE Direct SubmissionJOURNAL Exported Sep 26, 2017 from MiaoLingPlasmidhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..5992/organism="synthetic DNA construct"/mol_type="other DNA"enhancer 235..614/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 615..818/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"promoter 863..881/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"CDS 927..1541/codon_start=1/label=AAP2/translation="METQTQYLTPSLSDSHQQPPLVWELIRWLQAVAHQWQTITRAPTEWVIPREIGIAIPHGWATESSPPAPEPGPCPPTTTTSTNKFPANQEPRTTITTLATAPLGGILTSTDSTATFHHVTGKDSSTTTGDSDPRDSTSSSLTFKSKRSRRMTVRRRLPITLPARFRCLLTRSTSSRTSSARRIKDASRRSQQTSSWCHSMDTSP"polyA_signal 1592..1816/label=bGH poly(A) signal/note="bovine growth hormone polyadenylation signal"rep_origin 1862..2290/direction=RIGHT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 2304..2633/label=SV40 promoter/note="SV40 enhancer and early promoter"rep_origin 2484..2619/label=SV40 ori/note="SV40 origin of replication"CDS 2700..3494/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=Neo/note="confers resistance to neomycin, kanamycin, and G418(Geneticin(R))"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"polyA_signal 3668..3789/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"rep_origin complement(4240..4825)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(4996..5856)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(5857..5961)/gene="bla"/label=AmpR promoterORIGIN1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc901 gtttaaacgg gccctctaga ctcgagatgg agacgcagac tcagtacctg acccccagcc961 tctcggacag ccaccagcag ccccctctgg tctgggaact aatacgatgg ctacaggcag1021 tggcgcacca atggcagaca ataacgaggg cgccgacgga gtgggtaatt cctcgggaaa1081 ttggcattgc gattccacat ggatgggcga cagagtcatc accaccagca cccgaacctg1141 ggccctgccc acctacaaca accacctcta caaacaaatt tccagccaat caggagcctc1201 gaacgacaat cactactttg gctacagcac cccttggggg tattttgact tcaacagatt1261 ccactgccac ttttcaccac gtgactggca aagactcatc aacaacaact ggggattccg1321 acccaagaga ctcaacttca agctctttaa cattcaagtc aaagaggtca cgcagaatga1381 cggtacgacg acgattgcca ataaccttac cagcacggtt caggtgttta ctgactcgga1441 gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga tgcctcccgc cgttcccagc1501 agacgtcttc atggtgccac agtatggata cctcaccctg aggatccgag ctcggtacca1561 agcttaagtt taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt1621 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc1681 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt1741 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat1801 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc1861 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc1921 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc1981 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt2041 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg2101 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt2161 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta2221 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt2281 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc2341 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt2401 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca2461 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc2521 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg2581 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc2641 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca2701 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg2761 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag2821 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc2881 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc2941 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg3001 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc3061 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca3121 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag3181 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg3241 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg3301 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca3361 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc3421 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg3481 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct3541 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt3601 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc3661 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa3721 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa3781 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc3841 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg3901 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt3961 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg4021 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga4081 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat4141 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca4201 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc4261 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata4321 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
