• 规格：20μl质粒
• 质粒图谱1： png/1-1F924194532208.png

质粒序列： 点击查看序列

LOCUS       Exported                6357 bp ds-DNA     circular SYN 24-SEP-2017

DEFINITION  synthetic circular DNA

KEYWORDS    psiCHECK2-let-7 4x

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 6357)

  TITLE     Direct Submission

  JOURNAL   Exported Sep 24, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..6357

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        62..419

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      270..405

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     intron          489..621

                     /label=chimeric intron

                     /note="chimera between introns from human beta-globin and 

                     immunoglobulin heavy chain genes"

     promoter        666..684

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     CDS             694..1629

                     /codon_start=1

                     /product="Renilla luciferase"

                     /label=hRluc

                     /note="human codon-optimized"

                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN

                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW

                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI

                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE

                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV

                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"

     misc_feature    1644..1751

                     /label=let-7 4x

     polyA_signal    1772..1820

                     /note="synthetic polyadenylation signal"

     promoter        1829..2580

                     /label=HSV TK promoter

                     /note="herpes simplex virus thymidine kinase promoter"

     CDS             2616..4268

                     /codon_start=1

                     /label=hluc+

                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA

                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP

                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS

                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA

                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK

                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY

                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS

                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL

                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV

                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"

     polyA_signal    4312..4433

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     promoter        4566..4670

                     /gene="bla"

                     /label=AmpR promoter

     CDS             4671..5531

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      5702..6290

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct

       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag

      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg

      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag

      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc

      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg

      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa

      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca

      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct

      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc

      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga

      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag

      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg

      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt

      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc

      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat

      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac

     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac

     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg

     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa

     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag

     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag

     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc

     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat

     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga

     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag

     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac

     1621 gagcagtaat tctaggcgat cgctcgagac tatacaagga tctacctcag tcgagactat

     1681 acaaggatct acctcagtcg agactataca aggatctacc tcagtcgaga ctatacaagg

     1741 atctacctca gtcgacgcgg ccgctggccg caataaaata tctttatttt cattacatct

     1801 gtgtgttggt tttttgtgtg aggatctaaa tgagtcttcg gacctcgcgg gggccgctta

     1861 agcggtggtt agggtttgtc tgacgcgggg ggagggggaa ggaacgaaac actctcattc

     1921 ggaggcggct cggggtttgg tcttggtggc cacgggcacg cagaagagcg ccgcgatcct

     1981 cttaagcacc cccccgccct ccgtggaggc gggggtttgg tcggcgggtg gtaactggcg

     2041 ggccgctgac tcgggcgggt cgcgcgcccc agagtgtgac cttttcggtc tgctcgcaga

     2101 cccccgggcg gcgccgccgc ggcggcgacg ggctcgctgg gtcctaggct ccatggggac

     2161 cgtatacgtg gacaggctct ggagcatccg cacgactgcg gtgatattac cggagacctt

     2221 ctgcgggacg agccgggtca cgcggctgac gcggagcgtc cgttgggcga caaacaccag

     2281 gacggggcac aggtacacta tcttgtcacc cggaggcgcg agggactgca ggagcttcag

     2341 ggagtggcgc agctgcttca tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc

     2401 ggaagaaata tatttgcatg tctttagttc tatgatgaca caaaccccgc ccagcgtctt

     2461 gtcattggcg aattcgaaca cgcagatgca gtcggggcgg cgcggtccca ggtccacttc

     2521 gcatattaag gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg acccgcttaa

     2581 aagcttggca ttccggtact gttggtaaag ccaccatggc cgatgctaag aacattaaga

     2641 agggccctgc tcccttctac cctctggagg atggcaccgc tggcgagcag ctgcacaagg

     2701 ccatgaagag gtatgccctg gtgcctggca ccattgcctt caccgatgcc cacattgagg

     2761 tggacatcac ctatgccgag tacttcgaga tgtctgtgcg cctggccgag gccatgaaga

     2821 ggtacggcct gaacaccaac caccgcatcg tggtgtgctc tgagaactct ctgcagttct

     2881 tcatgccagt gctgggcgcc ctgttcatcg gagtggccgt ggcccctgct aacgacattt

     2941 acaacgagcg cgagctgctg aacagcatgg gcatttctca gcctaccgtg gtgttcgtgt

     3001 ctaagaaggg cctgcagaag atcctgaacg tgcagaagaa gctgcctatc atccagaaga

     3061 tcatcatcat ggactctaag accgactacc agggcttcca gagcatgtac acattcgtga

     3121 catctcatct gcctcctggc ttcaacgagt acgacttcgt gccagagtct ttcgacaggg

     3181 acaaaaccat tgccctgatc atgaacagct ctgggtctac cggcctgcct aagggcgtgg

     3241 ccctgcctca tcgcaccgcc tgtgtgcgct tctctcacgc ccgcgaccct attttcggca

     3301 accagatcat ccccgacacc gctattctga gcgtggtgcc attccaccac ggcttcggca

     3361 tgttcaccac cctgggctac ctgatttgcg gctttcgggt ggtgctgatg taccgcttcg

     3421 aggaggagct gttcctgcgc agcctgcaag actacaaaat tcagtctgcc ctgctggtgc

     3481 caaccctgtt cagcttcttc gctaagagca ccctgatcga caagtacgac ctgtctaacc

     3541 tgcacgagat tgcctctggc ggcgccccac tgtctaagga ggtgggcgaa gccgtggcca

     3601 agcgctttca tctgccaggc atccgccagg gctacggcct gaccgagaca accagcgcca

     3661 ttctgattac cccagagggc gacgacaagc ctggcgccgt gggcaaggtg gtgccattct

     3721 tcgaggccaa ggtggtggac ctggacaccg gcaagaccct gggagtgaac cagcgcggcg

     3781 agctgtgtgt gcgcggccct atgattatgt ccggctacgt gaataaccct gaggccacaa

     3841 acgccctgat cgacaaggac ggctggctgc actctggcga cattgcctac tgggacgagg

     3901 acgagcactt cttcatcgtg gaccgcctga agtctctgat caagtacaag ggctaccagg

     3961 tggccccagc cgagctggag tctatcctgc tgcagcaccc taacattttc gacgccggag

     4021 tggccggcct gcccgacgac gatgccggcg agctgcctgc cgccgtcgtc gtgctggaac

     4081 acggcaagac catgaccgag aaggagatcg tggactatgt ggccagccag gtgacaaccg

     4141 ccaagaagct gcgcggcgga gtggtgttcg tggacgaggt gcccaagggc ctgaccggca

     4201 agctggacgc ccgcaagatc cgcgagatcc tgatcaaggc taagaaaggc ggcaagatcg

     4261 ccgtgtaata attctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca

     4321 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa

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