- 商品货号:ZT1543
- 规格:10μl质粒
- 启动子:MCMV promoter
- 复制子:pUC ori
- 终止子:SV40 poly(A) signal
- 质粒分类:病毒系列质粒;慢病毒类质粒;病毒表达质粒
- 质粒大小:3913bp
- 原核抗性:氨苄青霉素Amp
- 克隆菌株:大肠杆菌Stbl3
- 5测序引物:pDC315-F:acgtgggtataagaggcg
- 3测序引物:pDC315-R:cgatgctagacgatccag
- 质粒简介: pDC315质粒是一种真核表达质粒载体,将目的基因插入多克隆位点,转染细胞即可进行表达。 带有目的基因的 pDC315 可以通过插入位点两侧的酶切位点以及目的基因内部的特异酶切位点进行鉴定。
1. Ampr : 为质粒在 E.coli(如 DH5α)中扩增提供氨苄抗性(终浓度 100µg/ml)。 2. ori:质粒在 E.coli 中的复制起点。
3. Ad:Ad 序列,主要由 Ad 可以与 Ad 基因组质粒发生同源重组。
4. ITR:Ad 反向末端重复序列,是 Ad 基因组的包装识别信号。
5. MCMV:来自小鼠 CMV 病毒的立早启动子。
6. SV40 polyA:来自 SV40 病毒 T 抗原的 mRNA 加尾信号。
7. loxP:真核重组酶(Cre)的重组识别序列。- 质粒图谱1: png/1-1F91PZ214928.png
质粒序列: 点击查看序列LOCUS Exported 3913 bp ds-DNA circular SYN 18-SEP-2017DEFINITION synthetic circular DNASOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 3913)TITLE Direct SubmissionJOURNAL Exported Sep 18, 2017 from MiaoLingPlasmidhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..3913/organism="synthetic DNA construct"/mol_type="other DNA"misc_feature complement(2..102)/label=ITRmisc_signal 190..340/label=Ad5 Psi/note="packaging signal for adenovirus serotype 5"misc_feature 469..991/label=MCMV promotermisc_feature 993..1028/label=MCSpolyA_signal 1029..1188/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"protein_bind 1190..1223/label=loxP/bound_moiety="Cre recombinase"/note="Cre-mediated recombination occurs in the 8-bp coresequence (GCATACAT)."rep_origin complement(1647..2235)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(2406..3266)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(3267..3371)/gene="bla"/label=AmpR promotermisc_feature 3454..453/label=Admisc_feature 3823..1/label=ITRORIGIN1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcaa gctgctagca aggatccagc1021 ttgtcgactt cgagcaactt gtttattgca gcttataatg gttacaaata aagcaatagc1081 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa1141 ctcatcaatg tatcttatca tgtctggatc gtctagcatc gaagatccaa taacttcgta1201 tagcatacat tatacgaagt tataagtagc ttggcgtaat catggtcata gctgtttcct1261 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt1321 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc1381 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg1441 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg1501 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca1561 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac1621 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac1681 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg1741 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac1801 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat1861 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag1921 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac1981 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt2041 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt2101 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc2161 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga2221 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac2281 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc2341 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct2401 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca2461 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct2521 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca2581 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc2641 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg2701 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct2761 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa2821 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta2881 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc2941 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg3001 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa3061 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg3121 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc3181 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg3241 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat3301 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata3361 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc3421 atgacattaa cctataaaaa taggcgtatc actctaggca aaatagcacc ctcccgctcc3481 agaacaacat acagcgcttc acagcggcag cctaacagtc agccttacca gtaaaaaaga3541 aaacctatta aaaaaacacc actcgacacg gcaccagctc aatcagtcac agtgtaaaaa3601 agggccaagt gcagagcgag tatatatagg actaaaaaat gacgtaacgg ttaaagtcca3661 caaaaaacac ccagaaaacc gcacgcgaac ctacgcccag aaacgaaagc caaaaaaccc3721 acaacttcct caaatcgtca cttccgtttt cccacgttac gtaacttccc attttaagaa3781 aactacaatt cccaacacat acaagttact ccgccctaaa acctacgtca cccgccccgt3841 tcccacgccc cgcgccacgt cacaaactcc accccctcat tatcatattg gcttcaatcc3901 aaaataaggt ata//
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