- 规格:20μl质粒
- 启动子:CYC1 promoter
- 复制子:pUC ori,F1 ori,2μ ori
- 终止子:CYC1 terminator
- 质粒分类:酵母细胞质粒;酵母杂交质粒;双杂交类质粒
- 质粒大小:7599bp
- 质粒标签:C-Cub,C-LexA,VP16
- 原核抗性:卡那霉素Kan
- 筛选标记:LEU2
- 克隆菌株:DH5α等大肠杆菌
- 表达宿主:酵母菌
- 备注:高拷贝
- 质粒简介: pBT3-C是一个用于膜蛋白酵母双杂交实验的质粒。The DUALmembrane system is intended for the detection and identification of interactions involving integral membrane proteins and membrane-associated proteins in yeast. The DUALmembrane pairwise interaction kit enables you to:
Investigate the interaction between a membrane protein (integral or membrane-associated) and a membrane protein or soluble protein
Map domains or amino acids which are critical for an interaction
Screen cDNA libraries using a membrane protein as a bait to find novel
interacting proteins (requires purchase of a separate cDNA library from Dualsystems)