• 规格：20μl质粒
• 启动子：CMV promoter
• 终止子：SV40 poly(A) signal
• 质粒大小：13661bp
• 质粒标签：C-EGFP
• 原核抗性：卡那霉素Kan
• 筛选标记：新霉素Neo/G418
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：          1136- Desmoplakin-GFP质粒是一个哺乳细胞过表达质粒，CMV启动子驱动Desmoplakin人源基因和EGFP绿色荧光蛋白融合表达。该质粒的参考文献是：Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. 
• 质粒图谱1： png/1-1G02516434b53.png

质粒序列： 点击查看序列

LOCUS       Exported               13661 bp ds-DNA     circular SYN 25-OCT-2017

DEFINITION  synthetic circular DNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 13661)

  AUTHORS   .

  TITLE     Direct Submission

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..13661

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        68..371

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        372..575

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     misc_feature    704..982

                     /label=DP insert 5'region

     misc_feature    983..9600

                     /label=Desmoplakin

     CDS             9614..10333

                     /codon_start=1

                     /product="enhanced GFP"

                     /label=EGFP

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     polyA_signal    10456..10577

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(10584..11039)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        11066..11170

                     /gene="bla"

                     /label=AmpR promoter

     promoter        11172..11529

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      11380..11515

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             11564..12358

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    12590..12637

                     /label=HSV TK poly(A) signal

                     /note="herpes simplex virus thymidine kinase 

                     polyadenylation signal (Cole and Stacy, 1985)"

     rep_origin      12966..13554

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg

       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc

      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt

      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc

      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg

      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg

      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact

      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa

      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta

      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct

      601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt cgcggccgcg tcaacagccc

      661 ctgctccttg ggcccctcca tgccatgccg taatctctcc cacccgacca acaccaacac

      721 ccagctccga cgcagctcct ctgcgccctt gccgccctcc gagccacagc tttcctcccg

      781 ctcctgcccc cggcccgtcg ccgtctccgc gctcgcagcg gcctcgggag ggcccaggta

      841 gcgagcagcg acctcgcgag ccttccgcac tcccgcccgg ttccccggcc gtccgcctat

      901 ccttggcccc ctccgctttc tccgcgccgg cccgcctcgc ttatgcctcg gcgctgagcc

      961 gctctcccga ttgcccgccg acatgagctg caacggaggc tcccacccgc ggatcaacac

     1021 tctgggccgc atgatccgcg ccgagtctgg cccggacctg cgctacgagg tgaccagcgg

     1081 cggcgggggc accagcagga tgtactattc tcggcgcggc gtgatcaccg accagaactc

     1141 ggacggctac tgtcaaaccg gcacgatgtc caggcaccag aaccagaaca ccatccagga

     1201 gctgctgcag aactgctccg actgcttgat gcgagcagag ctcatcgtgc agcctgaatt

     1261 gaagtatgga gatggaatac aactgactcg gagtcgagaa ttggatgagt gttttgccca

     1321 ggccaatgac caaatggaaa tcctcgacag cttgatcaga gagatgcggc agatgggcca

     1381 gccctgtgat gcttaccaga aaaggcttct tcagctccaa gagcaaatgc gagcccttta

     1441 taaagccatc agtgtccctc gagtccgcag ggccagctcc aagggtggtg gaggctacac

     1501 ttgtcagagt ggctctggct gggatgagtt caccaaacat gtcaccagtg aatgtttggg

     1561 gtggatgagg cagcaaaggg cggagatgga catggtggcc tggggtgtgg acctggcctc

     1621 agtggagcag cacattaaca gccaccgggg catccacaac tccatcggcg actatcgctg

     1681 gcagctggac aaaatcaaag ccgacctgcg cgagaaatct gcgatctacc agttggagga

     1741 ggagtatgaa aacctgctga aagcgtcctt tgagaggatg gatcacctgc gacagctgca

     1801 gaacatcatt caggccacgt ccagggagat catgtggatc aatgactgcg aggaggagga

     1861 gctgctgtac gactggagcg acaagaacac caacatcgct cagaaacagg aggccttctc

     1921 catacgcatg agtcaactgg aagttaaaga aaaagagctc aataagctga aacaagaaag

     1981 tgaccaactt gtcctcaatc agcatccagc ttcagacaaa attgaggcct atatggacac

     2041 tctgcagacg cagtggagtt ggattcttca gatcaccaag tgcattgatg ttcatctgaa

     2101 agaaaatgct gcctactttc agttttttga agaggcgcag tctactgaag catacctgaa

     2161 ggggctccag gactccatca ggaagaagta cccctgcgac aagaacatgc ccctgcagca

     2221 cctgctggaa cagatcaagg agctggagaa agaacgagag aaaatccttg aatacaagcg

     2281 tcaggtgcag aacttggtaa acaagtctaa gaagattgta cagctgaagc ctcgtaaccc

     2341 agactacaga agcaataaac ccattattct cagagctctc tgtgactaca aacaagatca

     2401 gaaaatcgtg cataaggggg atgagtgtat cctgaaggac aacaacgagc gcagcaagtg

     2461 gtacgtgacg ggcccgggag gcgttgacat gcttgttccc tctgtggggc tgatcatccc

     2521 tcctccgaac ccactggccg tggacctctc ttgcaagatt gagcagtact acgaagccat

     2581 cttggctctg tggaaccagc tctacatcaa catgaagagc ctggtgtcct ggcactactg

     2641 catgattgac atagagaaga tcagggccat gacaatcgcc aagctgaaaa caatgcggca

     2701 ggaagattac atgaagacga tagccgacct tgagttacat taccaagagt tcatcagaaa

     2761 tagccaaggc tcagagatgt ttggagatga tgacaagcgg aaaatacagt ctcagttcac

     2821 cgatgcccag aagcattacc agaccctggt cattcagctc cctggctatc cccagcacca

     2881 gacagtgacc acaactgaaa tcactcatca tggaacctgc caagatgtca accataataa

     2941 agtaattgaa accaacagag aaaatgacaa gcaagaaaca tggatgctga tggagctgca

     3001 gaagattcgc aggcagatag agcactgcga gggcaggatg actctcaaaa acctccctct

     3061 agcagaccag gggtcttctc accacatcac agtgaaaatt aacgagctta agagtgtgca

     3121 gaatgattca caagcaattg ctgaggttct caaccagctt aaagatatgc ttgccaactt

     3181 cagaggttct gaaaagtact gctatttaca gaatgaagta tttggactat ttcagaaact

     3241 ggaaaatatc aatggtgtta cagatggcta cttaaatagc ttatgcacag taagggcact

     3301 gctccaggct attctccaaa cagaagacat gttaaaggtt tatgaagcca ggctcactga

     3361 ggaggaaact gtctgcctgg acctggataa agtggaagct taccgctgtg gactgaagaa

     3421 aataaaaaat gacttgaact tgaagaagtc gttgttggcc actatgaaga cagaactaca

     3481 gaaagcccag cagatccact ctcagacttc acagcagtat ccactttatg atctggactt

     3541 gggcaagttc ggtgaaaaag tcacacagct gacagaccgc tggcaaagga tagataaaca

     3601 gatcgacttt agattatggg acctggagaa acaaatcaag caattgagga attatcgtga

     3661 taactatcag gctttctgca agtggctcta tgatgctaaa cgccgccagg attccttaga

     3721 atccatgaaa tttggagatt ccaacacagt catgcggttt ttgaatgagc agaagaactt

     3781 gcacagtgaa atatctggca aacgagacaa atcagaggaa gtacaaaaaa ttgctgaact

     3841 ttgcgccaat tcaattaagg attatgagct ccagctggcc tcatacacct caggactgga

     3901 aactctgctg aacataccta tcaagaggac catgattcag tccccttctg gggtgattct

     3961 gcaagaggct gcagatgttc atgctcggta cattgaacta cttacaagat ctggagacta

     4021 ttacaggttc ttaagtgaga tgctgaagag tttggaagat ctgaagctga aaaataccaa

     4081 gatcgaagtt ttggaagagg agctcagact ggcccgagat gccaactcgg aaaactgtaa

     4141 taagaacaaa ttcctggatc agaacctgca gaaataccag gcagagtgtt cccagttcaa

     4201 agcgaagctt gcgagcctgg aggagctgaa gagacaggct gagctggatg ggaagtcggc

     4261 taagcaaaat ctagacaagt gctacggcca aataaaagaa ctcaatgaga agatcacccg

     4321 actgacttat gagattgaag atgaaaagag aagaagaaaa tctgtggaag acagatttga

     4381 ccaacagaag aatgactatg accaactgca gaaagcaagg caatgtgaaa aggagaacct

     4441 tggttggcag aaattagagt ctgagaaagc catcaaggag aaggagtacg agattgaaag

     4501 gttgagggtt ctactgcagg aagaaggcac ccggaagaga gaatatgaaa atgagctggc

     4561 aaaggtaaga aaccactata atgaggagat gagtaattta aggaacaagt atgaaacaga

     4621 gattaacatt acgaagacca ccatcaagga gatatccatg caaaaagagg atgattccaa

     4681 aaatcttaga aaccagcttg atagactttc aagggaaaat cgagatctga aggatgaaat

     4741 tgtcaggctc aatgacagca tcttgcaggc cactgagcag cgaaggcgag ctgaagaaaa

     4801 cgcccttcag caaaaggcct gtggctctga gataatgcag aagaagcagc atctggagat

     4861 agaactgaag caggtcatgc agcagcgctc tgaggacaat gcccggcaca agcagtccct

     4921 ggaggaggct gccaagacca ttcaggacaa aaataaggag atcgagagac tcaaagctga

     4981 gtttcaggag gaggccaagc gccgctggga atatgaaaat gaactgagta aggtaagaaa

     5041 caattatgat gaggagatca ttagcttaaa aaatcagttt gagaccgaga tcaacatcac