- 商品货号:FH1602
- 规格:10μl质粒
- 启动子:CMV promoter
- 终止子:SV40 poly(A) signal
- 质粒大小:13661bp
- 质粒标签:C-EGFP
- 原核抗性:卡那霉素Kan
- 筛选标记:新霉素Neo/G418
- 克隆菌株:大肠杆菌DH5α
- 质粒简介: 1136- Desmoplakin-GFP质粒是一个哺乳细胞过表达质粒,CMV启动子驱动Desmoplakin人源基因和EGFP绿色荧光蛋白融合表达。该质粒的参考文献是:Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin.
- 质粒图谱1: png/1-1G02516434b53.png
质粒序列: 点击查看序列LOCUS Exported 13661 bp ds-DNA circular SYN 25-OCT-2017DEFINITION synthetic circular DNASOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 13661)AUTHORS .TITLE Direct Submissionhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..13661/organism="synthetic DNA construct"/mol_type="other DNA"enhancer 68..371/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 372..575/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"misc_feature 704..982/label=DP insert 5'regionmisc_feature 983..9600/label=DesmoplakinCDS 9614..10333/codon_start=1/product="enhanced GFP"/label=EGFP/note="mammalian codon-optimized"/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"polyA_signal 10456..10577/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"rep_origin complement(10584..11039)/direction=LEFT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 11066..11170/gene="bla"/label=AmpR promoterpromoter 11172..11529/label=SV40 promoter/note="SV40 enhancer and early promoter"rep_origin 11380..11515/label=SV40 ori/note="SV40 origin of replication"CDS 11564..12358/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=NeoR/KanR/note="confers resistance to neomycin, kanamycin, and G418(Geneticin(R))"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"polyA_signal 12590..12637/label=HSV TK poly(A) signal/note="herpes simplex virus thymidine kinasepolyadenylation signal (Cole and Stacy, 1985)"rep_origin 12966..13554/direction=RIGHT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"ORIGIN1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt cgcggccgcg tcaacagccc661 ctgctccttg ggcccctcca tgccatgccg taatctctcc cacccgacca acaccaacac721 ccagctccga cgcagctcct ctgcgccctt gccgccctcc gagccacagc tttcctcccg781 ctcctgcccc cggcccgtcg ccgtctccgc gctcgcagcg gcctcgggag ggcccaggta841 gcgagcagcg acctcgcgag ccttccgcac tcccgcccgg ttccccggcc gtccgcctat901 ccttggcccc ctccgctttc tccgcgccgg cccgcctcgc ttatgcctcg gcgctgagcc961 gctctcccga ttgcccgccg acatgagctg caacggaggc tcccacccgc ggatcaacac1021 tctgggccgc atgatccgcg ccgagtctgg cccggacctg cgctacgagg tgaccagcgg1081 cggcgggggc accagcagga tgtactattc tcggcgcggc gtgatcaccg accagaactc1141 ggacggctac tgtcaaaccg gcacgatgtc caggcaccag aaccagaaca ccatccagga1201 gctgctgcag aactgctccg actgcttgat gcgagcagag ctcatcgtgc agcctgaatt1261 gaagtatgga gatggaatac aactgactcg gagtcgagaa ttggatgagt gttttgccca1321 ggccaatgac caaatggaaa tcctcgacag cttgatcaga gagatgcggc agatgggcca1381 gccctgtgat gcttaccaga aaaggcttct tcagctccaa gagcaaatgc gagcccttta1441 taaagccatc agtgtccctc gagtccgcag ggccagctcc aagggtggtg gaggctacac1501 ttgtcagagt ggctctggct gggatgagtt caccaaacat gtcaccagtg aatgtttggg1561 gtggatgagg cagcaaaggg cggagatgga catggtggcc tggggtgtgg acctggcctc1621 agtggagcag cacattaaca gccaccgggg catccacaac tccatcggcg actatcgctg1681 gcagctggac aaaatcaaag ccgacctgcg cgagaaatct gcgatctacc agttggagga1741 ggagtatgaa aacctgctga aagcgtcctt tgagaggatg gatcacctgc gacagctgca1801 gaacatcatt caggccacgt ccagggagat catgtggatc aatgactgcg aggaggagga1861 gctgctgtac gactggagcg acaagaacac caacatcgct cagaaacagg aggccttctc1921 catacgcatg agtcaactgg aagttaaaga aaaagagctc aataagctga aacaagaaag1981 tgaccaactt gtcctcaatc agcatccagc ttcagacaaa attgaggcct atatggacac2041 tctgcagacg cagtggagtt ggattcttca gatcaccaag tgcattgatg ttcatctgaa2101 agaaaatgct gcctactttc agttttttga agaggcgcag tctactgaag catacctgaa2161 ggggctccag gactccatca ggaagaagta cccctgcgac aagaacatgc ccctgcagca2221 cctgctggaa cagatcaagg agctggagaa agaacgagag aaaatccttg aatacaagcg2281 tcaggtgcag aacttggtaa acaagtctaa gaagattgta cagctgaagc ctcgtaaccc2341 agactacaga agcaataaac ccattattct cagagctctc tgtgactaca aacaagatca2401 gaaaatcgtg cataaggggg atgagtgtat cctgaaggac aacaacgagc gcagcaagtg2461 gtacgtgacg ggcccgggag gcgttgacat gcttgttccc tctgtggggc tgatcatccc2521 tcctccgaac ccactggccg tggacctctc ttgcaagatt gagcagtact acgaagccat2581 cttggctctg tggaaccagc tctacatcaa catgaagagc ctggtgtcct ggcactactg2641 catgattgac atagagaaga tcagggccat gacaatcgcc aagctgaaaa caatgcggca2701 ggaagattac atgaagacga tagccgacct tgagttacat taccaagagt tcatcagaaa2761 tagccaaggc tcagagatgt ttggagatga tgacaagcgg aaaatacagt ctcagttcac2821 cgatgcccag aagcattacc agaccctggt cattcagctc cctggctatc cccagcacca2881 gacagtgacc acaactgaaa tcactcatca tggaacctgc caagatgtca accataataa2941 agtaattgaa accaacagag aaaatgacaa gcaagaaaca tggatgctga tggagctgca3001 gaagattcgc aggcagatag agcactgcga gggcaggatg actctcaaaa acctccctct3061 agcagaccag gggtcttctc accacatcac agtgaaaatt aacgagctta agagtgtgca3121 gaatgattca caagcaattg ctgaggttct caaccagctt aaagatatgc ttgccaactt3181 cagaggttct gaaaagtact gctatttaca gaatgaagta tttggactat ttcagaaact3241 ggaaaatatc aatggtgtta cagatggcta cttaaatagc ttatgcacag taagggcact3301 gctccaggct attctccaaa cagaagacat gttaaaggtt tatgaagcca ggctcactga3361 ggaggaaact gtctgcctgg acctggataa agtggaagct taccgctgtg gactgaagaa3421 aataaaaaat gacttgaact tgaagaagtc gttgttggcc actatgaaga cagaactaca3481 gaaagcccag cagatccact ctcagacttc acagcagtat ccactttatg atctggactt3541 gggcaagttc ggtgaaaaag tcacacagct gacagaccgc tggcaaagga tagataaaca3601 gatcgacttt agattatggg acctggagaa acaaatcaag caattgagga attatcgtga3661 taactatcag gctttctgca agtggctcta tgatgctaaa cgccgccagg attccttaga3721 atccatgaaa tttggagatt ccaacacagt catgcggttt ttgaatgagc agaagaactt3781 gcacagtgaa atatctggca aacgagacaa atcagaggaa gtacaaaaaa ttgctgaact3841 ttgcgccaat tcaattaagg attatgagct ccagctggcc tcatacacct caggactgga3901 aactctgctg aacataccta tcaagaggac catgattcag tccccttctg gggtgattct3961 gcaagaggct gcagatgttc atgctcggta cattgaacta cttacaagat ctggagacta4021 ttacaggttc ttaagtgaga tgctgaagag tttggaagat ctgaagctga aaaataccaa4081 gatcgaagtt ttggaagagg agctcagact ggcccgagat gccaactcgg aaaactgtaa4141 taagaacaaa ttcctggatc agaacctgca gaaataccag gcagagtgtt cccagttcaa4201 agcgaagctt gcgagcctgg aggagctgaa gagacaggct gagctggatg ggaagtcggc4261 taagcaaaat ctagacaagt gctacggcca aataaaagaa ctcaatgaga agatcacccg4321 actgacttat gagattgaag atgaaaagag aagaagaaaa tctgtggaag acagatttga4381 ccaacagaag aatgactatg accaactgca gaaagcaagg caatgtgaaa aggagaacct4441 tggttggcag aaattagagt ctgagaaagc catcaaggag aaggagtacg agattgaaag4501 gttgagggtt ctactgcagg aagaaggcac ccggaagaga gaatatgaaa atgagctggc4561 aaaggtaaga aaccactata atgaggagat gagtaattta aggaacaagt atgaaacaga4621 gattaacatt acgaagacca ccatcaagga gatatccatg caaaaagagg atgattccaa4681 aaatcttaga aaccagcttg atagactttc aagggaaaat cgagatctga aggatgaaat4741 tgtcaggctc aatgacagca tcttgcaggc cactgagcag cgaaggcgag ctgaagaaaa4801 cgcccttcag caaaaggcct gtggctctga gataatgcag aagaagcagc atctggagat4861 agaactgaag caggtcatgc agcagcgctc tgaggacaat gcccggcaca agcagtccct4921 ggaggaggct gccaagacca ttcaggacaa aaataaggag atcgagagac tcaaagctga4981 gtttcaggag gaggccaagc gccgctggga atatgaaaat gaactgagta aggtaagaaa5041 caattatgat gaggagatca ttagcttaaa aaatcagttt gagaccgaga tcaacatcac服务时间:24小时在线客服
客服热线:400-000-7085
丰晖生物
中国优质生物资源平台