• 规格：20μl质粒
• 启动子：CMV
• 复制子：pUC
• 终止子：bGH
• 原核抗性：氨苄青霉素Amp
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：             pCDNA3.1-EEF2-Myc-6×His(NM_001961.3)是一个人源基因哺乳细胞过表达质粒。
• 质粒图谱1： png/1-1G213160513137.png

质粒序列： 点击查看序列

LOCUS       Exported                8085 bp ds-DNA     circular SYN 13-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..8085

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        235..614

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        615..818

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     promoter        863..881

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     regulatory      960..969

                     /regulatory_class="other"

                     /note="vertebrate consensus sequence for strong initiation 

                     of translation (Kozak, 1987)"

     misc_feature    966..3539

                     /label=EEF2（NM_001961.3）

     CDS             3570..3599

                     /codon_start=1

                     /product="Myc (human c-Myc proto-oncogene) epitope tag"

                     /label=Myc

                     /translation="EQKLISEEDL"

     CDS             3615..3632

                     /codon_start=1

                     /product="6xHis affinity tag"

                     /label=6xHis

                     /translation="HHHHHH"

     polyA_signal    3682..3906

                     /label=bGH poly(A) signal

                     /note="bovine growth hormone polyadenylation signal"

     rep_origin      3952..4380

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        4394..4723

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      4574..4709

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             4790..5584

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=Neo

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    5758..5879

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(6330..6918)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(7089..7949)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(7950..8054)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg

       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg

      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc

      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt

      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata

      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc

      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc

      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt

      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt

      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca

      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg

      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc

      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg

      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca

      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc

      901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcg

      961 ccaccatggt gaacttcacg gtagaccaga tccgcgccat catggacaag aaggccaaca

     1021 tccgcaacat gtctgtcatc gcccacgtgg accatggcaa gtccacgctg acagactccc

     1081 tggtgtgcaa ggcgggcatc atcgcctcgg cccgggccgg ggagacacgc ttcactgata

     1141 cccggaagga cgagcaggag cgttgcatca ccatcaagtc aactgccatc tccctcttct

     1201 acgagctctc ggagaatgac ttgaacttca tcaagcagag caaggacggt gccggcttcc

     1261 tcatcaacct cattgactcc cccgggcatg tcgacttctc ctcggaggtg actgctgccc

     1321 tccgagtcac cgatggcgca ttggtggtgg tggactgcgt gtcaggcgtg tgcgtgcaga

     1381 cggagacagt gctgcggcag gccattgccg agcgcatcaa gcctgtgctg atgatgaaca

     1441 agatggaccg cgccctgctg gagctgcagc tggagcccga ggagctctac cagactttcc

     1501 agcgcatcgt ggagaacgtg aacgtcatca tctccaccta cggcgagggc gagagcggcc

     1561 ccatgggcaa catcatgatc gatcctgtcc tcggtaccgt gggctttggg tctggcctcc

     1621 acgggtgggc cttcaccctg aagcagtttg ccgagatgta tgtggccaag ttcgccgcca

     1681 agggggaggg ccagttgggg cctgccgagc gggccaagaa agtagaggac atgatgaaga

     1741 agctgtgggg tgacaggtac tttgacccag ccaacggcaa gttcagcaag tcagccacca

     1801 gccccgaagg gaagaagctg ccacgcacct tctgccagct gatcctggac cccatcttca

     1861 aggtgtttga tgcgatcatg aatttcaaga aagaggagac agcaaaactg atagagaaac

     1921 tggacatcaa actggacagc gaggacaagg acaaagaagg caaacccctg ctgaaggctg

     1981 tgatgcgccg ctggctgcct gccggagacg ccttgttgca gatgatcacc atccacctgc

     2041 cctcccctgt gacggcccag aagtaccgct gcgagctcct gtacgagggg cccccggacg

     2101 acgaggctgc catgggcatt aaaagctgtg accccaaagg ccctcttatg atgtatattt

     2161 ccaaaatggt gccaacctcc gacaaaggtc ggttctacgc ctttggacga gtcttctcgg

     2221 ggctggtctc cactggcctg aaggtcagga tcatggggcc caactatacc cctgggaaga

     2281 aggaggacct ctacctgaag ccaatccaga gaacaatctt gatgatgggc cgctacgtgg

     2341 agcccatcga ggatgtgcct tgtgggaaca ttgtgggcct cgtgggcgtg gaccagttcc

     2401 tggtgaagac gggcaccatc accaccttcg agcacgcgca caacatgcgg gtgatgaagt

     2461 tcagcgtcag ccctgttgtc agagtggccg tggaggccaa gaacccggct gacctgccca

     2521 agctggtgga ggggctgaag cggctggcca agtccgaccc catggtgcag tgcatcatcg

     2581 aggagtcggg agagcatatc atcgcgggcg ccggcgagct gcacctggag atctgcctga

     2641 aggacctgga ggaggaccac gcctgcatcc ccatcaagaa atctgacccg gtcgtctcgt

     2701 accgcgagac ggtcagtgaa gagtcgaacg tgctctgcct ctccaagtcc cccaacaagc

     2761 acaaccggct gtacatgaag gcgcggccct tccccgacgg cctggccgag gacatcgata

     2821 aaggcgaggt gtccgcccgt caggagctca agcagcgggc gcgctacctg gccgagaagt

     2881 acgagtggga cgtggctgag gcccgcaaga tctggtgctt tgggcccgac ggcaccggcc

     2941 ccaacatcct caccgacatc accaagggtg tgcagtacct caacgagatc aaggacagtg

     3001 tggtggccgg cttccagtgg gccaccaagg agggcgcact gtgtgaggag aacatgcggg

     3061 gtgtgcgctt cgacgtccac gacgtcaccc tgcacgccga cgccatccac cgcggagggg

     3121 gccagatcat ccccacagca cggcgctgcc tctatgccag tgtgctgacc gcccagccac

     3181 gcctcatgga gcccatctac cttgtggaga tccagtgtcc agagcaggtg gtcggtggca

     3241 tctacggggt tttgaacagg aagcggggcc acgtgttcga ggagtcccag gtggccggca

     3301 cccccatgtt tgtggtcaag gcctatctgc ccgtcaacga gtcctttggc ttcaccgctg

     3361 acctgaggtc caacacgggc ggccaggcgt tcccccagtg tgtgtttgac cactggcaga

     3421 tcctgcccgg agaccccttc gacaacagca gccgccccag ccaggtggtg gcggagaccc

     3481 gcaagcgcaa gggcctgaaa gaaggcatcc ctgccctgga caacttcctg gacaaattgg

     3541 gatccgagct cggtaccaag cttgggcccg aacaaaaact catctcagaa gaggatctga

     3601 atagcgccgt cgaccatcat catcatcatc attgagttta aacggtctcc agcttaagtt

     3661 taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct

     3721 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg

     3781 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc

     3841 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct

     3901 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct

     3961 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg

     4021 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg

     4081 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac

     4141 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct

     4201 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt

     4261 tccaaactgg aacaacactc aaccctatct cggtctat