• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：                 pUC57s-Lox-m(356-1236)(NM_010728.3)是一个小鼠基因截短克隆质粒。
• 质粒图谱1： png/1-1P12120133E10.png

质粒序列： 点击查看序列

LOCUS       Exported                3488 bp ds-DNA     circular SYN 21-JAN-2018

FEATURES             Location/Qualifiers

     source          1..3488

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     primer_bind     379..395

                     /label=M13 fwd

                     /note="common sequencing primer, one of multiple similar 

                     variants"

     misc_feature    444..1196

                     /label=Lox-m(356-1236)

     primer_bind     complement(1267..1283)

                     /label=M13 rev

                     /note="common sequencing primer, one of multiple similar 

                     variants"

     rep_origin      complement(1669..2257)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(2428..3288)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(3289..3393)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc

      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat

      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt

      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ggagatcggt acttcgcgaa

      421 tgcgtcgaga tcccattgaa ttcggcgacg acccctacaa tccctacaag tactccgacg

      481 acaaccccta ttataactac tatgacacgt atgagaggcc ccggcccggg agcaggaacc

      541 gacctggata cggcaccggt tacttccagt acggtctccc ggacctggtg cccgacccct

      601 actacatcca ggcttccacg tacgtccaga agatgtctat gtacaacctg agatgcgctg

      661 cggaagaaaa ctgcctggcc agttcagcat atagggcgga tgtcagagac tatgaccaca

      721 gggtactgct acgatttccg caaagagtga agaaccaagg gacatcggac ttcttaccaa

      781 gccgccctcg gtactcctgg gagtggcaca gctgtcacca acattaccac agcatggacg

      841 agttcagcca ctatgacctg cttgatgcca acacacagag gagagtggct gaaggccaca

      901 aagcaagctt ctgtctggag gacacgtcct gtgactatgg gtaccacagg cgctttgcgt

      961 gcactgcaca cacacaggga ttgagtcctg gatgttatga cacctatgcg gcagacatag

     1021 actgccagtg gattgatatt acagatgtac aacctggaaa ctacattcta aaggtcagtg

     1081 taaaccccag ctacctggtg cctgaatcag actacactaa caatgttgta cgctgtgaca

     1141 ttcgctacac aggacatcat gcctatgcct caggctgcac aatttcaccg tattagagtc

     1201 gaccccatta tcggatgccg ggaccgacga gtgcagaggc gtgcaagcga gcttggcgta

     1261 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat

     1321 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt

     1381 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta

     1441 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc

     1501 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa

     1561 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa

     1621 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct

     1681 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac

     1741 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc

     1801 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc

     1861 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg

     1921 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga

     1981 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag

     2041 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta

     2101 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag

     2161 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg

     2221 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac

     2281 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc

     2341 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag

     2401 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc

     2461 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac

     2521 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc

     2581 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg

     2641 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag

     2701 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc

     2761 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac

     2821 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag

     2881 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac

     2941 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg

     3001 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc

     3061 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact

     3121 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg

     3181 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa

     3241 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt

     3301 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg

     3361 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga

     3421 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc

     3481 ctttcgtc

//