• 规格：20μl质粒
• 启动子：CMV
• 质粒分类：哺乳动物细胞慢病毒表达载体，shRNA，RNA干扰载体
• 质粒大小：7881 bp
• 原核抗性：Amp
• 筛选标记：ZsGreen1
• 克隆菌株：Stbl3
• 备注：含有组成型人U6启动子的慢病毒shRNA载体

质粒序列： 点击查看序列

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatctgggc aggaagaggg cctatttccc atgattcctt 2221 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa 2281 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg 2341 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt 2401 tcgatttctt ggctttatat atcttgtgga aaggacgagg atccggacaa gcttcgaatt 2461 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc 2521 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat 2581 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc 2641 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc 2701 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt 2761 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta 2821 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg 2881 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac 2941 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg 3001 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct 3061 accggtcgcc accatggccc agtccaagca cggcctgacc aaggagatga ccatgaagta 3121 ccgcatggag ggctgcgtgg acggccacaa gttcgtgatc accggcgagg gcatcggcta 3181 ccccttcaag ggcaagcagg ccatcaacct gtgcgtggtg gagggcggcc ccttgccctt 3241 cgccgaggac atcttgtccg ccgccttcat gtacggcaac cgcgtgttca ccgagtaccc 3301 ccaggacatc gtcgactact tcaagaactc ctgccccgcc ggctacacct gggaccgctc 3361 cttcctgttc gaggacggcg ccgtgtgcat ctgcaacgcc gacatcaccg tgagcgtgga 3421 ggagaactgc atgtaccacg agtccaagtt ctacggcgtg aacttccccg ccgacggccc 3481 cgtgatgaag aagatgaccg acaactggga gccctcctgc gagaagatca tccccgtgcc 3541 caagcagggc atcttgaagg gcgacgtgag catgtacctg ctgctgaagg acggtggccg 3601 cttgcgctgc cagttcgaca ccgtgtacaa ggccaagtcc gtgccccgca agatgcccga 3661 ctggcacttc atccagcaca agctgacccg cgaggaccgc agcgacgcca agaaccagaa 3721 gtggcacctg accgagcacg ccatcgcctc cggctccgcc ttgccctaac tcgagtctag 3781 agggcccgac gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact 3841 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 3901 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 3961 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 4021 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 4081 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 4141 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg 4201 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 4261 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 4321 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc 4381 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa 4441 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg 4501 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg 4561 tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac 4621 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc 4681 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc 4741 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt 4801 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt 4861 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga 4921 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg 4981 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt 5041 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 5101 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 5161 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 5221 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa 5281 tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac ctctagctag 5341 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 5401 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 5461 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 5521 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 5581 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 5641 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 5701 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 5761 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 5821 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 5881 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 5941 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 6001 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 6061 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 6121 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 6181 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 6241 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 6301 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 6361 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 6421 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 6481 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 6541 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 6601 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 6661 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 6721 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 6781 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 6841 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 6901 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 6961 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 7021 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 7081 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 7141 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 7201 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 7261 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 7321 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 7381 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 7441 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 7501 gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta 7561 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 7621 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca 7681 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct 7741 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg 7801 tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata 7861 tgtactacaa acttagtagt //