- 规格:20μl质粒
- 启动子:CMV
- 质粒分类:腺病毒载体
- 质粒大小:6.1 kb
- 原核抗性:Amp
- 克隆菌株:Stbl3
- 备注:The vector pAAV-IRES-hrGFP contains a dicistronic expression cassette in which the Renilla reniformis green fluorescent protein (hrGFP) gene is expressed as a second open reading frame downstream of the encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES). The hrGFP gene serves as a useful marker for titering the virus and ascertaining the infection efficiency for the desired target cell type. Another unique feature of this vector is the FLAG sequence (3x) at the c-terminal of MCS. The foreign gene could be cloned in frame with the tag and the protein could be further purified or detected by anti-FLAG antibody.