- 规格:20μl质粒
- 启动子:SV40
- 质粒分类:哺乳细胞表达;启动子增强子报告系统
- 质粒大小:5256 bp
- 原核抗性:Amp
- 克隆菌株:DH5α
- 备注:pGL3-control是荧光素酶报告载体,含有SV40 增强子和启动子,增强荧光素酶的表达。
质粒序列: 点击查看序列1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta 61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc 181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 241 caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata 301 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat 361 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc 421 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg 481 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa 541 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac 601 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc 661 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa 721 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc 781 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat 841 agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt 901 gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt 961 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt 1021 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga 1081 tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg 1141 gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct 1201 aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt 1261 gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca 1321 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca 1381 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga 1441 ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg 1501 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac 1561 gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat 1621 caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca 1681 ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg 1741 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca 1801 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc 1861 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag 1921 atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac 1981 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa 2041 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac 2101 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc 2161 aagtaaaacc tctacaaatg tggtaaaatc gataaggatc tgaacgatgg agcggagaat 2221 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg 2281 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc 2341 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc 2401 ctggggactt tccacaccct aactgacaca cattccacag cggatccgtc gaccgatgcc 2461 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc 2521 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt 2581 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 2641 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 2701 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 2761 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 2821 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 2881 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 2941 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 3001 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 3061 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 3121 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 3181 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 3241 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 3301 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 3361 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 3421 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 3481 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 3541 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 3601 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 3661 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 3721 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 3781 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 3841 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 3901 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 3961 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 4021 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 4081 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 4141 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 4201 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 4261 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 4321 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 4381 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 4441 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 4501 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 4561 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 4621 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 4681 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 4741 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 4801 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 4861 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 4921 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcccat tcgccattca 4981 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagccca 5041 agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg gccgcaataa 5101 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac 5161 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag 5221 tgcaagtgca ggtgccagaa catttctcta tcgata //