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| 发货规格 |
1×10⁶ |
| 别名 |
HS 683; HS-683; Hs-683; Hs683; HS683; Hs 683.T; HS 683T; Hs683T |
| 形态 |
成纤维细胞样 |
| 生长特性 |
贴壁 |
| 培养条件 |
DMEM+10%FBS+1%P/S |
| 冻存条件 |
90%FBS+10%DMSO |
| 传代方式 |
胰酶消化 |
| 倍增时间 |
40~50h |
| 传代比例 |
1:2~1:4 |
| 换液频率 |
每周2~3次 |
| 生物安全等级 |
1 |
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| 种属 |
人源 |
| 组织来源 |
脑 |
| 供体信息 |
76岁男性白人 |
| 背景描述 |
HS-683 是一种人神经胶质瘤细胞系，来源于诊断为多形性胶质母细胞瘤的成年患者的脑组织。多形性胶质母细胞瘤是一种高度侵袭性的脑癌，以其快速生长和不良预后而闻名。HS-683 细胞系在癌症研究中很有价值，因为它能够深入了解驱动神经胶质瘤增殖、侵袭和对治疗的耐药性的分子机制。 |
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| 致瘤性 |
No; No， in immunosuppressed mice Yes， in semisolid medium |
| 核型 |
modal number = 82; range = 74 to 87. This is a hypertriploid human cell line with the modal chromosome number of 82 occurring in 16% of cells， with the distribution skewed toward the lower number range. Higher ploidies occurred in 3.0% of cells. Most cells had 4-6 marker chromosomes， but only del(3) (q12.2q21) occurred frequently in most cells， and der(9)t(9;?) (q34.2;?)， tdic(1;4) (q21;q35)， and a minute acrocentric occurred in more than two cells. N7 occurred in 4-6 copies per cell. Two X and one or two Y chromosomes were present in most cells. |
| 倍体 |
AK-1， 1 ES-D， 1 G6PD， B GLO-I， 2 Me-2， 2 PGM1， 1 PGM3， 1-2 |
