• 规格：20μl质粒
• 启动子：59 promoter
• 质粒分类：乳酸杆菌分泌表达质粒
• 质粒大小：8328 bp
• 质粒标签：Psusp
• 原核抗性：Amp
• 筛选标记：红霉素Erythromycin
• 克隆菌株：DH5α
• 表达宿主：乳酸杆菌

质粒序列： 点击查看序列

1 aactcaacgc tagtagtgga tttaatccca aatgagccaa cagaaccaga accagaaaca 61 gaatcagaac aagtaacatt ggatttagaa atggaagaag aaaaaagcaa tgacttcgtg 121 tgaataatgc acgaaatcgt tgcttatttt tttttaaaag cggtatacta gatataacga 181 aacaacgaac tgaatagaaa cgaaaaaaga gccatgacac atttataaaa tgtttgacga 241 cattttataa atgcatagcc cgataagatt gccaaaccaa cgcttatcag ttagtcagat 301 gaactcttcc ctcgtaagaa gttatttaat taactttgtt tgaagacggt atataaccgt 361 actatcatta tatagggaaa tcagagagtt ttcaagtatc taagctactg aatttaagaa 421 ttgttaagca atcaatcgga aatcgtttga ttgctttttt tgtattcatt tatagaaggt 481 ggagtttgta tgaatcatga tgaatgtaaa acttatataa aaaatagttt attggagata 541 agaaaattag caaatatcta tacactagaa acgtttaaga aagagttaga aaagagaaat 601 atctacttag aaacaaaatc agataagtat ttttcttcgg agggggaaga ttatatatat 661 aagttaatag aaaataacaa aataatttat tcgattagtg gaaaaaaatt gacttataaa 721 ggaaaaaaat ctttttcaaa acatgcaata ttgaaacagt tgaatgaaaa agcaaaccaa 781 gttaattaaa caacctattt tataggattt ataggaaagg agaacagctg aatgaatatc 841 ccttttgttg tagaaactgt gcttcatgac ggcttgttaa agtacaaatt taaaaatagt 901 aaaattcgct caatcactac caagccaggt aaaagcaaag gggctatttt tgcgtatcgc 961 tcaaaatcaa gcatgattgg cggtcgtggt gttgttctga cttccgagga agcgattcaa 1021 gaaaatcaag atacatttac acattggaca cccaacgttt atcgttatgg aacgtatgca 1081 gacgaaaacc gttcatacac gaaaggacat tctgaaaaca atttaagaca aatcaatacc 1141 ttctttattg attttgatat tcacacggca aaagaaacta tttcagcaag cgatatttta 1201 acaaccgcta ttgatttagg ttttatgcct actatgatta tcaaatctga taaaggttat 1261 caagcatatt ttgttttaga aacgccagtc tatgtgactt caaaatcaga atttaaatct 1321 gtcaaagcag ccaaaataat ttcgcaaaat atccgagaat attttggaaa gtctttgcca 1381 gttgatctaa cgtgtaatca ttttggtatt gctcgcatac caagaacgga caatgtagaa 1441 ttttttgatc ctaattaccg ttattctttc aaagaatggc aagattggtc tttcaaacaa 1501 acagataata agggctttac tcgttcaagt ctaacggttt taagcggtac agaaggcaaa 1561 aaacaagtag atgaaccctg gtttaatctc ttattgcacg aaacgaaatt ttcaggagaa 1621 aagggtttaa tagggcgtaa taacgtcatg tttaccctct ctttagccta ctttagttca 1681 ggctattcaa tcgaaacgtg cgaatataat atgtttgagt ttaataatcg attagatcaa 1741 cccttagaag aaaaagaagt aatcaaaatt gttagaagtg cctattcaga aaactatcaa 1801 ggggctaata gggaatacat taccattctt tgcaaagctt gggtatcaag tgatttaacc 1861 agtaaagatt tatttgtccg tcaagggtgg tttaaattca agaaaaaaag aagcgaacgt 1921 caacgtgttc atttgtcaga atggaaagaa gatttaatgg cttatattag cgaaaaaagc 1981 gatgtataca agccttattt agtgacgacc aaaaaagaga ttagagaagt gctaggcatt 2041 cctgaacgga cattagataa attgctgaag gtactgaagg cgaatcagga aattttcttt 2101 aagattaaac caggaagaaa tggtggcatt caacttgcta gtgttaaatc attgttgcta 2161 tcgatcatta aagtaaaaaa agaagaaaaa gaaagctata taaaggcgct gacaaattct 2221 tttgacttag agcatacatt cattcaagag actttaaaca agctagcaga acgccctaaa 2281 acggacacac aactcgattt gtttagctat gatacaggct gaaaataaaa cccgcactat 2341 gccattacat ttatatctat gatacgtgtt tgttttttct ttgctgttta gcgaatgatt 2401 agcagaaata tacagagtaa gattttaatt aattattagg gggagaagga gagagtagcc 2461 cgaaaacttt tagttggctt ggactgaacg aagtgaggga aaggctacta aaacgtcgag 2521 gggcagtgag agcgaagcga acacttgatt ttttaatttt ctatctttta taggtcatta 2581 gagtatactt atttgtccta taaactattt agcagcataa tagatttatt gaataggtca 2641 tttaagttga gcatattaga ggaggaaaat cttggagaaa tatttgaaga acccgattac 2701 atggattgga ttagttcttg tggttacgtg gtttttaact aaaagtagtg aatttttgat 2761 ttttggtgtg tgtgtcttgt tgttagtatt tgctagtcaa agtgattaaa tagaattcac 2821 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 2881 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 2941 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta 3001 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg 3061 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt 3121 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc 3181 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat 3241 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg 3301 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 3361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 3421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 3481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 3541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 3601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 3661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 3721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 3781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 3841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 3901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 3961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 4021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 4081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 4141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 4201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 4261 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 4321 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 4381 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 4441 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 4501 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 4561 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 4621 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 4681 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 4741 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 4801 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 4861 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 4921 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 4981 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 5041 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 5101 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 5161 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 5221 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 5281 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 5341 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 5401 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctcgaaatta 5461 accctcacta aagggaacaa aagctgggta ccgggccccc cctcgacggt atcgatagcc 5521 cgcctaatga gcgggctttt ttttgatccc ccaaagaagc gcgtaatatc aggtttaaga 5581 gacaataaga aaaaaagatt gaaaaaagtg acattaaatt cttgacaggg agagataggt 5641 ttgatagaat ataataggtt tgatagaata taatagggta ccgagctcga attcctgcag 5701 cccaaatcgc gaaaccgaac ttaatgggag gaaaaattaa aaaagaacag ttatgaaaaa 5761 aaagattatc tcagctattt taatgtctac agtgatactt tctgctgcag ccccgttgtc 5821 aggtgtttac gctgacacaa actcagatat tgctaaacaa gatgcggtcg acccattgtc 5881 aactaaaaaa ttacataaag aacctgcgac tttaattaaa gcgattgatg gtgatacggt 5941 taaattaatg tacaaaggtc aaccaatgac attcagacta ttattggttg atacacctga 6001 aacaaagcat cctaaaaaag gtgtagagaa atatggtcct gaagcaagtg catttacgaa 6061 aaaaatggta gaaaatgcaa agaaaattga agtcgagttt gacaaaggtc aaagaactga 6121 taaatatgga cgtggcttag cgtatattta tgctgatgga aaaatggtaa acgaagcttt 6181 agttcgtcaa ggcttggcta aagttgctta tgtttacaaa cctaacaata cacatgaaca 6241 acatttaaga aaaagtgaag cacaagcgaa aaaagagaaa ttaaatattt ggagcgaaga 6301 caacgctgat tcaggtcaaa tcgatctcga gatatcgcta gttctagatt gaggcatcaa 6361 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 6421 aacgctctcc tgagtaggac aaatccgccg ctctagctaa gcagaaggcc atcctgacgg 6481 atggcctttt tgcgtttcta caaactcttg ttggggatcc gcctgcagcc aagcttatcg 6541 attctagact cgagagctct cgagtctaga atcgatacga ttttgaagtg gcaacagata 6601 aaaaaaagca gtttaaaatt gttgctgaac ttttaaaaca agcaaataca atcattgtcg 6661 caacagatag cgacagagaa ggcgaaaaca ttgcctggtc gatcattcat aaagcaaatg 6721 ccttttctaa agataaaacg tataaaagac tatggatcaa tagtttagaa aaagatgtga 6781 tccgtagcgg ttttcaaaat ttgcaaccag gaatgaatta ctatcccttt tatcaagaag 6841 cgcaaaagaa aaacgaaatg atacaccaat cagtgcaaaa aaagatataa tgggagataa 6901 gacggttcgt gttcgtgctg acttgcacca tatcataaaa atcgaaacag caaagaatgg 6961 cggaaacgta aaagaagtta tggaaataag acttagaagc aaacttaaga gtgtgttgat 7021 agtgcagtat cttaaaattt tgtataatag gaattgaagt taaattagat gctaaaaatt 7081 tgtaattaag aaggagtgat tacatgaaca aaaatataaa atattctcaa aactttttaa 7141 cgagtgaaaa agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg 7201 tttacgaaat tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac 7261 aggtaacgtc tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac 7321 tgaatactcg tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac 7381 agaggtataa aattgttggg agtattcctt accatttaag cacacaaatt attaaaaaag 7441 tggtttttga aagccatgcg tctgacatct atctgattgt tgaagaagga ttctacaagc 7501 gtaccttgga tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc 7561 aattgcttaa gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa 7621 taaaacttac ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact 7681 ttgtttcaaa atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc 7741 aagcaatgaa acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt 7801 ctatttttaa tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt 7861 aaatttggaa agttacacgt tactaaaggg aatgtagata aattattagg tatactactg 7921 acagcttcca aggagctaaa gaggtcccta gcgcttagaa tcgctttagg aaacacgatc 7981 cagtccaata atcgtcgata aaaacttttg aaaaaggttg gtgaaattac ctacttttgg 8041 aataatcaca aatcacaagt gattaatcac aaatcacaag tgattaatca cttgtttatt 8101 aagatattaa aagctataat ttaaataaag cgtgaatttt attacacaaa aagagggggg 8161 agaaacttgg aactagcatt tagagaaagc ttaaaaaaga tgagaggtac caaatcaaaa 8221 gaaaaattct cccaagaatt agaaatgagt agatcaaatt attcacgaat agaatcagga 8281 aaatcagatc caaccataaa aacactagaa caaattgcaa agttaact