• 规格：20μl质粒
• 启动子：T7
• 质粒分类：大肠杆菌表达载体
• 质粒大小：5605 bp
• 质粒标签：N-His
• 原核抗性：Kan
• 克隆菌株：DH5α
• 表达宿主：Rosetta-gami2 、 Rosetta-gami2(DE3)
• 备注：与pET47系列载体相同，但多了Trx标签。; 含有HRV 3C蛋白酶切位点，可以在低温下将融合标签切除。含有C端Thrombin蛋白酶切位点

质粒序列： 点击查看序列

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgtttagcag cctaggttaa ttaagcctcg agagcagcag aagtagagct gtccatgtgc 181 tggcgttcga atttagcagc agcggtttct ttactaccgc gtggcaccag agcgagctct 241 gcggccgcaa gcttgtcgac ggacgtcggg cgcgccaagg cctgtacaga attcggatcc 301 tggtacccgg gtccctgaaa gaggacttca agagccgcgg agtgatggtg atggtgatga 361 ccagaaccac tagatggagt aggagtagga ggattgttat tagaaccgcc accaccacta 421 gtatggccag aaccagaacc ggccaggtta gcgtcgagga actctttcaa ctgaccttta 481 gacagtgcac ccactttggt tgccgccact tcaccgtttt tgaacagcag cagagtcggg 541 ataccacgga tgccatattt cggcgcagtg ccagggtttt gatcgatgtt cagttttgca 601 acggtcagtt tgccctgata ttcgtcagcg atttcatcca gaatcggggc gatcattttg 661 cacggaccgc accactctgc ccagaaatcg acgaggatcg ccccgtccgc tttgagtaca 721 tccgtgtcaa aactgtcgtc agtcaggtga ataattttat cgctcatatg tatatctcct 781 tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta 841 tagtgagtcg tattaatttc gcgggatcga gatcgatctc gatcctctac gccggacgca 901 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca 961 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta 1021 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc 1081 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt 1141 cgcataaggg agagcgtcga gatcccggac accatcgaat ggcgcaaaac ctttcgcggt 1201 atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg 1261 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 1321 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 1381 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 1441 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 1501 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 1561 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 1621 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 1681 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 1741 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 1801 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 1861 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 1921 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 1981 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt 2041 gcggacatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 2101 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 2161 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 2221 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 2281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 2341 taatgtaagt tagctcactc attaggcacc gggatctcga ccgatgccct tgagagcctt 2401 caacccagtc agctccttcc ggtgggcgcg gggcatgact agcatgatcg tgctcctgtc 2461 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat 2521 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg 2581 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac 2641 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc 2701 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca 2761 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac 2821 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc 2881 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt 2941 tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca 3001 ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc 3061 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 3121 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 3181 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt 3241 aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg tgtgaaatac 3301 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg 3361 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 3421 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 3481 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 3541 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 3601 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 3661 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 3721 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 3781 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 3841 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 3901 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 3961 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 4021 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 4081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 4141 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgaacaat aaaactgtct 4201 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc 4261 tctaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc 4321 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca 4381 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc 4441 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact 4501 cctgatgatg catggttact caccactgcg atccccggca aaacagcatt ccaggtatta 4561 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg 4621 ttgcattcga ttcctgtttg taattgtcct tttaacagtg atcgcgtatt tcgtctcgct 4681 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt 4741 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg 4801 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa 4861 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc 4921 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa 4981 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt 5041 ttctaagaat taattcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 5101 aggggttccg cgcacatttc cccgaaaagt gccacctgaa attgtaaacg ttaatatttt 5161 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 5221 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 5281 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 5341 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 5401 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 5461 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 5521 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 5581 gctacagggc gcgtcccatt cgcca