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| 发货规格 |
1×10⁶ |
| 别名 |
MDA-MB 453; MDA MB 453; MDA-MB453; MDAMB453; MDA-453; MDA453; MD Anderson-Metastatic Breast-453 |
| 形态 |
上皮细胞样 |
| 生长特性 |
贴壁 |
| 培养条件 |
L-15+10%FBS+1%P/S |
| 冻存条件 |
90%FBS+10%DMSO |
| 传代方式 |
胰酶消化 |
| 传代比例 |
1:2~1:4 |
| 换液频率 |
每周2~3次 |
| 生物安全等级 |
1 |
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| 种属 |
人源 |
| 组织来源 |
乳腺 |
| 肿瘤类型 |
乳腺癌 |
| 供体信息 |
48岁白人女性 |
| 背景描述 |
MDA-MB-453 由 R. Cailleau 等人于 1976 年从一名 48 岁女性乳腺癌转移性乳腺癌患者的积液中衍生出来，累及淋巴结、脑以及胸膜腔和心包腔。 |
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| 蛋白表达 |
Fibroblast growth factor (FGF)， expressed |
| 同工酶 |
AK-1， 1；ES-D， 1-2；G6PD， B；GLO-I， 1；PGM1， 1；PGM3， 1 |
| 致瘤性 |
No， in immunosuppressed mice；Yes， in semisolid medium |
| 核型 |
modal number = 90; range = 87 to 91.The cell line is aneuploid human female， with chromosome counts in the hypo- to near-tetraploid range. Normal chromosomes N11， N13， and N17 are absent， and chromosomes N2， N6， and N12 are clearly under-represented with respect to the copy number of other normal chromosomes， while chromosome N20 tends towards over-representation. A large number of marker chromosomes are present with consistency， many of them with more than one copy. The near-diploid population of cells reported for this culture has been replaced by a tetraploid population. |
