细胞信息
发货规格	1×10⁶
别名	SNU1; NCI-SNU-1
形态	上皮细胞样
生长特性	贴壁
培养条件	RPMI-1640+10%FBS+1%P/S
冻存条件	90%FBS+10%DMSO
传代方式	胰酶消化
传代比例	1:2~1:4
换液频率	每周2~3次
生物安全等级	1
细胞背景
种属	人源
组织来源	胃
肿瘤类型	胃癌
供体信息	44岁亚洲男性
背景描述	SNU-1 是一种表现出上皮形态的细胞系，是从一名 44 岁的亚洲男性癌症患者的胃中分离出来的。本品在癌症研究中有应用。
遗传背景
蛋白表达	Vasoactive intestinal peptide (VIP)， expressed
致癌基因	myc+; erb-B2+
致瘤性	Yes， reported colony forming efficiency of 1.9% in semisolid medium.
抗原表达	Blood Type O; Rh +，The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.
核型	SNU-1 is a near-diploid cell line with a modal chromosome number of 47; double minute chromosomes were found in 28% of the cells. They have a reported colony-forming efficiency of 1.9% in semisolid medium and a population doubling time of 26 hours. The cells grow as floating aggregates of round cell clusters. This is a hyperdiploid human cell line with the modal chromosome number of 47 occurring in 94% of cells. Higher ploidies occurred at 42%， which is very high. The karyotype of 47，XY，t(1;4) (q24;q33)， +M1 is very uniform and stable. M1 is an F- sized small metacentric chromosome.

STR鉴定

STR位点信息

STR鉴定图

Amelogenin	X,Y
CSF1PO	12,13 (ATCC=CRL-5971; Cosmic-CLP=908444) 12,14 (KCLB=00001)
D2S1338	23,24
D3S1358	16 (ATCC=CRL-5971) 16,17 (KCLB=00001)
D5S818	12,13 (ATCC=CRL-5971; Cosmic-CLP=908444) 13 (KCLB=00001)
D7S820	9,12
D8S1179	13,14
D13S317	11,14 (ATCC=CRL-5971; Cosmic-CLP=908444) 12,14 (KCLB=00001)
D16S539	9,12
D18S51	14,22
D19S433	13,14.2
D21S11	29,32.2
FGA	22 (KCLB=00001) 22,23 (ATCC=CRL-5971)
Penta D	10,11
Penta E	11,14
TH01	8,9.3
TPOX	8,10
vWA	14