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| 发货规格 |
1×10⁶ |
| 别名 |
T-84; T 84 |
| 形态 |
上皮细胞样 |
| 生长特性 |
贴壁 |
| 培养条件 |
DMEM/F12+5%FBS+1%P/S |
| 冻存条件 |
90%FBS+10%DMSO |
| 传代方式 |
胰酶消化 |
| 倍增时间 |
33.9h |
| 传代比例 |
1:2~1:4 |
| 换液频率 |
每周2~3次 |
| 生物安全等级 |
1 |
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| 种属 |
人源 |
| 组织来源 |
肠 |
| 肿瘤类型 |
肠腺癌 |
| 供体信息 |
72岁男性 |
| 背景描述 |
T84 是一种可移植的人癌细胞系，从一名 72 岁的男性结肠癌患者的肺转移中分离出来。该系在相邻细胞之间表现出紧密的连接和桥粒。细胞应保持在高密度培养，至少 1/4 汇合度。 |
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| 蛋白表达 |
Neurotransmitter， expressed; peptide hormone， expressed |
| 同工酶 |
AK-1， 1；ES-D， 1；G6PD， B；GLO-I， 1-2；Me-2， 1-2；PGM1， 1；PGM3， 1 |
| 致瘤性 |
Yes， in nude mice(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| 核型 |
The stemline modal chromosome number is 56， occurring at 28% with polyploidy at 12.4%. Eighteen markers are common to most metaphases examined. Normal x and chromosome 13 were absent， chromosomes 2， 4 and 22 were single-copied， and chromosome 12 was 4-copied.No Y chromosome was detected by Q band observation. DM occurred in nearly 50% of the cells. |
