• 规格：20μl质粒
• 质粒分类：细菌表达，CRISPR
• 质粒大小：9404 bp
• 原核抗性：Amp
• 克隆菌株：DH5α

质粒序列： 点击查看序列

agacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctagaggaccagccgcgtaacctggcaaaatcggttacggttgagtaataaatggatgccctgcgtaagcgggtgtgggcggacaataaagtcttaaactgaacaaaatagatctaaactatgacaataaagtcttaaactagacagaatagttgtaaactgaaatcagtccagttatgctgtgaaaaagcatactggacttttgttatggctaaagcaaactcttcattttctgaagtgcaaattgcccgtcgtattaaagaggggcgtggggttcgaggtcgacggtatcgataagctagcttaattagctgagcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagctagactgcgatgagtggcagggcggggcgtaatttttttaaggcagttattggtgcccttaaacgcctggggtaatgactctctagcttgaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgctaggagcttgcggcccggacgatcgaattggggatcttgaagtacctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctgatgtgcttaaaaacttactcaatggaattctagatcgaattggccgcggcgttgtgacaatttaccgaacaactccgcggccgggaagccgatctcggcttgaacgaattgttaggtggcggtacttgggtcgatatcaaagtgcatcacttcttcccgtatgcccaactttgtatagagagccactgcgggatcgtcaccgtaatctgcttgcacgtagatcacataagcaccaagcgcgttggcctcatgcttgaggagattgatgagcgcggtggcaatgccctgcctccggtgctcgccggagactgcgagatcatagatatagatctcactacgcggctgctcaaacttgggcagaacgtaagccgcgagagcgccaacaaccgcttcttggtcgaaggcagcaagcgcgatgaatgtcttactacggagcaagttcccgaggtaatcggagtccggctgatgttgggagtaggtggctacgtctccgaactcacgaccgaaaagatcaagagcagcccgcatggatttgacttggtcagggccgagcctacatgtgcgaatgatgcccatacttgagccacctaactttgttttagggcgactgccctgctgcgtaacatcgttgctgctgcgtaacatcgttgctgctccataacatcaaacatcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaaaaaaacagtcataacaagccatgaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctacttgcattacagtttacgaaccgaacaggcttatgtcaattcgatccattgctgttgacaaagggaatcaggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctaattcgatcatgcattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtaggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcaccattcgatggtgtcaacgtaaatgccgcttcgccttcgcgcgcgaattgcaagctgatccgggcttatcgactgcacggtgcaccaatgcttctggcgtcaggcagccatcggaagctgtggtatggctgtgcaggtcgtaaatcactgcataattcgtgtcgctcaaggcgcactcccgttctggataatgttttttgcgccgacatcataacggttctggcaaatattctgaaatgagctgtttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagaattcgagctcctgactcaggaggtatcgatatgtacccatacgatgttccagattacgctatgaccaacggcaagatcctgggcctggccatcggcatcgcctcggtgggcgtgggcatcatcgaggccaagaccggcaaggtggtgcacgccaactcgcgcctgttctcggccgccaacgccgagaacaacgccgagcgccgcggcttccgcggctcgcgccgcctgaaccgccgcaagaagcaccgcgtgaagcgcgtgcgcgacctgttcgagaagtacggcatcgtgaccgacttccgcaacctgaacctgaacccgtacgagctgcgcgtgaagggcctgaccgagcagctgaagaacgaggagctgttcgccgccctgcgcaccatctcgaagcgccgcggcatctcgtacctggacgacgccgaggacgactcgaccggctcgaccgactacgccaagtcgatcgacgagaaccgccgcctgctgaagaacaagaccccgggccagatccagctggagcgcctggagaagtacggccagctgcgcggcaacttcaccgtgtacgacgagaacggcgaggcccaccgcctgatcaacgtgttctcgacctcggactacgagaaggaggcccgcaagatcctggagacccaggccgactacaacaagaagatcaccgccgagttcatcgacgactacgtggagatcctgacccagaagcgcaagtactaccacggcccgggcaacgagaagtcgcgcaccgactacggccgcttccgcaccgacggcaccaccctggagaacatcttcggcatcctgatcggcaagtgcaacttctacccggacgagtaccgcgcctcgaaggcctcgtacaccgcccaggagtacaacttcctgaacgacctgaacaacctgaaggtgtcgaccgagaccggcaagctgtcgaccgagcagaaggagtcgctggtggagttcgccaagaacaccgccaccctgggcccggccaagctgctgaaggagatcgccaagatcctggactgcaaggtggacgagatcaagggctaccgcgaggacgacaagggcaagccggacctgcacaccttcgagccgtaccgcaagctgaagttcaacctggagtcgatcaacatcgacgacctgtcgcgcgaggtgatcgacaagctggccgacatcctgaccctgaacaccgagcgcgagggcatcgaggacgccatcaagcgcaacctgccgaaccagttcaccgaggagcagatctcggagatcatcaaggtgcgcaagtcgcagtcgaccgccttcaacaagggctggcactcgttctcggccaagctgatgaacgagctgatcccggagctgtacgccacctcggacgagcagatgaccatcctgacccgcctggagaagttcaaggtgaacaagaagtcgtcgaagaacaccaagaccatcgacgagaaggaggtgaccgacgagatctacaacccggtggtggccaagtcggtgcgccagaccatcaagatcatcaacgccgccgtgaagaagtacggcgacttcgacaagatcgtgatcgagatgccgcgcgacaagaacgccgacgacgagaagaagttcatcgacaagcgcaacaaggagaacaagaaggagaaggacgacgccctgaagcgcgccgcctacctgtacaactcgtcggacaagctgccggacgaggtgttccacggcaacaagcagctggagaccaagatccgcctgtggtaccagcagggcgagcgctgcctgtactcgggcaagccgatctcgatccaggagctggtgcacaactcgaacaacttcgagatcgacgccatcctgccgctgtcgctgtcgttcgacgactcgctggccaacaaggtgctggtgtacgcctggaccaaccaggagaagggccaaaagaccccgtaccaggtgatcgactcgatggacgccgcctggtcgttccgcgagatgaaggactacgtgctgaagcagaagggcctgggcaagaagaagcgcgactacctgctgaccaccgagaacatcgacaagatcgaggtgaagaagaagttcatcgagcgcaacctggtggacacccgctacgcctcgcgcgtggtgctgaactcgctgcagtcggccctgcgcgagctgggcaaggacaccaaggtgtcggtggtgcgcggccagttcacctcgcagctgcgccgcaagtggaagatcgacaagtcgcgcgagacctaccaccaccacgccgtggacgccctgatcatcgccgcctcgtcgcagctgaagctgtgggagaagcaggacaacccgatgttcgtggactacggcaagaaccaggtggtggacaagcagaccggcgagatcctgtcggtgtcggacgacgagtacaaggagctggtgttccagccgccgtaccagggcttcgtgaacaccatctcgtcgaagggcttcgaggacgagatcctgttctcgtaccaggtggactcgaagtacaaccgcaaggtgtcggacgccaccatctactcgacccgcaaggccaagatcggcaaggacaagaaggaggagacctacgtgctgggcaagatcaaggacatctactcgcagaacggcttcgacaccttcatcaagaagtacaacaaggacaagacccagttcctgatgtaccagaaggactcgctgacctgggagaacgtgatcgaggtgatcctgcgcgactacccgaccaccaagaagtcggaggacggcaagaacgacgtgaagtgcaacccgttcgaggagtaccgccgcgagaacggcctgatctgcaagtactcgaagaagggcaagggcaccccgatcaagtcgctgaagtactacgacaagaagctgggcaactgcatcgacatcaccccggaggagtcgcgcaacaaggtgatcctgcagtcgatcaacccgtggcgcgccgacgtgtacttcaacccggagaccctgaagtacgagctgatgggcctgaagtactcggacctgtcgttcgagaagggcaccggcaactaccacatctcgcaggagaagtacgacgccatcaaggagaaggagggcatcggcaagaagtcggagttcaagttcaccctgtaccgcaacgacctgatcctgatcaaggacatcgcctcgggcgagcaggagatctaccgcttcctgtcgcgcaccatgccgaacgtgaaccactacgtggagctgaagccgtacgacaaggagaagttcgacaacgtgcaggagctggtggaggccctgggcgaggccgacaaggtgggccgctgcatcaagggcctgaacaagccgaacatctcgatctacaaggtgcgcaccgacgtgctgggcaacaagtacttcgtgaagaagaagggcgacaagccgaagctggacttcaagaacaacaagaagtaaatactctggccatttttctttaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcaacgcgtgcggccgctgattagctaagcagaaggccatcctgacggatgcctttttcgcttgctagctgcagcccgggggatcccccgggctgcaggaattcctcgagaagcttgggcccggtacctcgcgaaggccttgcaggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacagcggggcatttttcttcctgttatgtttgggcgctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgacccgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccgg