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发货规格 |
1×10⁶ |
别名 |
Cv-1; CV 1; CV-1.K; CV1 |
形态 |
成纤维细胞样 |
生长特性 |
贴壁 |
培养条件 |
DMEM+10%FBS+1%P/S |
冻存条件 |
90%FBS+10%DMSO |
传代方式 |
胰酶消化 |
传代比例 |
1:2~1:4 |
换液频率 |
每周2~3次 |
生物安全等级 |
1 |
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种属 |
猴源 |
组织来源 |
肾 |
供体信息 |
141天雄性非洲绿猴 |
背景描述 |
CV-1 是 1964 年源自肾脏的非洲绿猴细胞系。这种成纤维细胞样细胞系最初用于专注于致癌性 Rous 肉瘤病毒 (RSV) 转化的研究,广泛用于病毒生产、转染和基因沉默的生物学研究。 |
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病毒易感性 |
Human poliovirus 1、Herpes simplex virus、Eastern equine encephalitis virus、Western equine encephalitis virus、California encephalitis virus、SV40 virus |
病毒耐药 |
Poliovirus 1, herpes simplex, Eastern equine encephalitis, Western equine encephalitis, California encephalitis, SV40 |
逆转录酶 |
Negative |
核型 |
This is a pseudodiploid, male African green monkey cell line. The modal chromosome number was 60, occurring in 48% of cells, and the rate of polyploidy was at 4.4%. Only a few markers were found., Of these M1, a probable deleted N11, was found in all cells examined; M3 of unknown origin was in some cells; and the remaining 2 to 3 others of unknown origins were found only once., N11 was uniformly single copied, and N16 was also single copied in most cells. Both X and Y chromosomes were also detected in every cell. |