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发货规格 |
1×10⁶ |
别名 |
Zr-75-1; ZR751; ZR75-1; ZR75.1; ZR75_1 |
形态 |
上皮细胞样 |
生长特性 |
贴壁 |
培养条件 |
RPMI-1640+10%FBS+1%P/S |
冻存条件 |
90%FBS+10%DMSO |
传代方式 |
胰酶消化 |
倍增时间 |
70~80h |
传代比例 |
1:2~1:4 |
换液频率 |
每周2~3次 |
生物安全等级 |
1 |
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种属 |
人源 |
组织来源 |
腹腔积液 |
肿瘤类型 |
乳腺癌 |
供体信息 |
63岁白人女性 |
背景描述 |
ZR-75-1 是一种上皮细胞系,是从一名 63 岁的白人女性导管癌患者的乳腺组织中分离出来的。该细胞系可用于癌症研究。 |
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蛋白表达 |
Estrogen receptor, expressed;The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene. |
同工酶 |
G6PD, B |
基因表达 |
mucin (apomucin, MUC-1, MUC-2) |
致瘤性 |
Yes, forms tumors in nude mice |
核型 |
This human cell line has a hypertriploid chromosome number. The modal chromosome number was 72 occurring in 26% of the cells and the rate of higher ploidies was at 1.2%. Eighteen markers were common.They are: t(1q,?), M2,M3, del(3) (p21), der(5)t(5;?) (q35;?), del(6) (q21), t(11q14q), t(11;11) (p15;q11), der(14)t(2;14) (q21;q32), t(17q,?), M13, M14, M15, M16, M17, der(8)t(8;?), t(9p,?) and 19pt. Of these M13 was paired. Normal N14 was not found. N6 was single copied, X had 3 copies and N18 had 4 copies in each cell. |