• 规格：10ul质粒
• • 质粒宿主：  大肠杆菌
  • 质粒用途：  包装辅助
  • 片段类型：  ORF
  • 片段物种：  
  • 原核抗性：  Chl
  • 筛选标记：  
  • 荧光标记：  
  • 启动子:  
  • 复制子:  
  • 拷贝数:  
  • 感受态:  DH5a
  • 温度:  37度
  • 背景载体:  
  • 正向引物:  P0488-F：
  • 反向引物:  p0488-R：GGTAGCTCAGAGAACCTTCG
  • 诱导方式:  

质粒序列： 点击查看序列

CGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCCTGGTGCTACGCCTGAATAAGTGATAATAAGCGGATGAATGGCAGAAATTCGAAGAATAGTTACGGCTTATGACATCTTTGTGGACACATCATTCACTTTTTATTCACATCCGGCCCTGAACTCGCTAGGACTTGCCCCGGTGCATTTTTTAAATACCCGCGAAAAATAGAGCTGATCGTCAAATCCAACATTGCGCCCAACGGTCGCTATCGGCATTCGCGTAGTGCTAAGCAGAAGTTTCGCCTGGCTGATACGCTGATCTTCGCGCCAGCTCAATACGCTAATGCCTAACTGCTGGCGGAACAGATGTGATAACCGGGAGGGCGACAGGCAGACATGCTGGGCGACGCTGGCGATATCAAAATGGCTGTCCGCCAGATGGTCGCTGATATACTGGCAGGCATCGCGCACACGGCTATCCATCGGCGGGTGCAACGACTCATTAATTACCGCCATACGTCTGAGCAACAACTGCTCCAGCAGATTGATCGCCAGTAGCTCAGAATAGCGACCTTCCCCTTGCCCGGCGCTGATGATCTGCCCGAACAGTTCGCTGAAATGCGGCTGGCGCGCCTCGTCCGGGCGGAAAAATCCTGTCTGGGCAAAGATTGTCGGCCAGGTCAGCCACTCCTGCCAGTAGGCGCGAGGCCGGAAATAAACCCACTGGTGATACCACTCGCTGGCGTCCGGATGCCGTCCATAGTGATGAATCTCGCCCGGCGGAAACAATAATATATCGCCAGGCCGACAGACAAACTGCTCGCCATTATTATTAATGACGCCCTCTCCGCGGATGGTCAGGTTAAGAATATATCCCTTCATGCCCAACGGACGATCGATAAAAAAATCCAGATATCCATTCGCTTCAATTGGCGTCAGCCCGGCGACCAGATGGGCATTAAATGAATATCCCGGCAATAGCGGATCATTTTGCGTTTCAGCCATGATTTCTCTACCCCCCGATGTTCAGAGAAGAAACAAATTGTCCATATCGACCAGGACGACAGAGCTTCCGTCTCCGCAAGACTTTGCGCTTGATGAAAGCACGTATCAACCCCGCTTGTGAAAAGCGCTTTGTAACAAAAGCGTACAGTTCAGGCGATAAAATTAAGTAACAGAAGTGTCTATAACTATGGCTGGAATGTCCACATTGAATATTTGCACAGCGTCACACTTTGCAAAGCATTAGCATTTTTGTCCATAAGATTAGCGGATCCTGCCTGACGGTTTTTGCCGCGACTCTCTATAATTTCTCCATACCTGTTTTTCTGGATGGAGTAAGACCATGGCCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACAAGCTGGGTGAAGACTTCACTTACTCTGTAGAGTTTGAAGTTTATCCGGAAGTTGAACTGCAGGGTCTGGAAGCGATCGAAGTTGAAAAACCGATCGTTGAAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGCGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGAACTGCCGCGCGAACTGTTCGAAGAACAGGCTAAACGCCGCGTAGTTGTTGGCCTGCTGCTGGGCGAAGTTATCCGCACCAACGAGCTGAAAGCTGACGAAGAGCGCGTGAAAGGCCTGATCGAAGAGATGGCTTCTGCGTACGAAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGCGTAACAACAAAAGATTGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACCCCATAGATCTGGTACCAAGCTTATCGATGATAAGCTGTCAAACATGAGAATTACAACTTATATCGTATGGGGCTGACTTCAGGTGCTACATTTGAAGAGATAAATTGCACTGAAATCTAGAAATATTTTATCTGATTAATAAGATGATCTTCTTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTTCGTAAGCCATTTCCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAATATATCCTGTATCACATATTCTGCTGACGCACCGGTGCAGCCTTTTTTCTCCTGCCACATGAAGCACTTCACTGACACCCTCATCAGTGCCAACATAGTAAGCCAGTATACACTCCGCTAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCGACGCACTTTGCGCCGAATAAATACCTGTGACGGAAGATCACTTCGCAGAATAAATAAATCCTGGTGTCCCTGTTGATACCGGGAAGCCCTGGGCCAACTTTTGGCGAAAATGAGACGTTGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTC