• 规格：20μl质粒
• 启动子：Lac/lac
• 复制子：pUC
• 质粒分类：大肠杆菌载体；克隆质粒
• 质粒大小：3197bp
• 质粒标签：LacZ
• 原核抗性：Amp
• 克隆菌株：DH5a

质粒序列： 点击查看序列

LOCUS Exported 3197 bp ds-DNA circular SYN 24-JUN-2015 DEFINITION Vector for standard cloning, efficient in vitro RNA synthesis, and ssDNA production. Identical to pGEM(R)?3Zf(-) except for the orientation of the f1 origin. ACCESSION X65306 VERSION . KEYWORDS pGEM-3Zf(+) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3197) AUTHORS Promega TITLE Direct Submission JOURNAL Exported Thursday, September 1, 2016 from SnapGene Viewer 3.1.4 http://www.fenghbio.cn FEATURES Location/Qualifiers source 1..3197 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" misc_feature 5..61 /note="MCS" /note="pUC18/19 multiple cloning site" promoter complement(68..86) /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(104..120) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 128..144 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(152..182) /note="lac promoter" /note="promoter for the E. coli lac operon" rep_origin complement(506..1094) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1265..2125) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2126..2230) /gene="bla" /note="AmpR promoter" rep_origin complement(2562..3017) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" CDS complement(2994..108) /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /note="lacZ-alpha" /translation="MTMITPSYLGDTIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWTRPVAAH" primer_bind 3158..3174 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" promoter 3181..2 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 gggcgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct 61 tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg 121 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 181 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 241 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 301 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 361 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 421 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 481 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 541 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 601 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 661 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 721 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 781 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 841 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 901 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 961 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 1021 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 1081 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 1141 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 1201 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 1261 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 1321 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 1381 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 1441 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 1501 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 1561 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 1621 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 1681 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 1741 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 1801 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 1861 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 1921 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 1981 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 2041 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 2101 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 2161 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 2221 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 2281 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 2341 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 2401 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 2461 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 2521 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat 2581 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 2641 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 2701 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 2761 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 2821 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 2881 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 2941 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 3001 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg 3061 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta 3121 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg 3181 taatacgact cactata //