- 商品货号:FH1673
- 规格:10μl质粒
- 原核抗性:氨苄青霉素Amp
- 克隆菌株:大肠杆菌Stbl3
- 质粒简介: SaBE4-Gam是一个大鼠基因哺乳细胞过表达质粒。
- 质粒图谱1: png/1-1G201162630647.png
质粒序列: 点击查看序列LOCUS Exported 8601 bp ds-DNA circular SYN 01-DEC-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..8601/organism="synthetic DNA construct"/mol_type="other DNA"promoter 132..335/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"promoter 377..395/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"regulatory 403..412/regulatory_class="other"/note="vertebrate consensus sequence for strong initiationof translation (Kozak, 1987)"misc_feature 409..930/label=GamCDS 979..1662/codon_start=1/product="cytidine deaminase (C to U editing enzyme) fromrat"/label=APOBEC-1/note="can use ssDNA as a substrate (Komor et al., 2016)"/translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGGRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLSRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNEAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHILWATGLK"misc_feature 1759..4917/label=SaCas9CDS 4924..4944/codon_start=1/product="nuclear localization signal of SV40 (simian virus40) large T antigen"/label=SV40 NLS/translation="PKKKRKV"CDS 4951..5016/codon_start=1/product="three tandem FLAG(R) epitope tags, followed by anenterokinase cleavage site"/label=3xFLAG/translation="DYKDHDGDYKDHDIDYKDDDDK"CDS 5047..5295/codon_start=1/product="uracil-DNA glycosylase inhibitor from a Bacillussubtilis bacteriophage (Mol et al., 1995)"/label=UGI/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"CDS 5326..5574/codon_start=1/product="uracil-DNA glycosylase inhibitor from a Bacillussubtilis bacteriophage (Mol et al., 1995)"/label=UGI/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"CDS 5587..5607/codon_start=1/product="nuclear localization signal of SV40 (simian virus40) large T antigen"/label=SV40 NLS/translation="PKKKRKV"CDS 5616..5633/codon_start=1/product="6xHis affinity tag"/label=6xHis/translation="HHHHHH"polyA_signal 5662..5886/label=bGH poly(A) signal/note="bovine growth hormone polyadenylation signal"rep_origin complement(6359..6947)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(7118..7978)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(7979..8083)/gene="bla"/label=AmpR promoterenhancer 8353..131/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"ORIGIN1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggctaaacca421 gcaaaacgta tcaagagtgc cgcagcggct tatgtgccac aaaaccgcga tgcggtgatt481 accgatatta aacgcatcgg ggatttacag cgcgaagcat cacgtctgga aacggaaatg541 aatgatgcca tcgcggaaat tacggagaaa tttgcggccc ggattgcacc gattaaaacc601 gatattgaaa ccctttcaaa aggcgttcag ggatggtgtg aagcgaaccg cgacgaactg661 acgaacggcg gcaaagtgaa gacggcgaat cttgtcaccg gtgatgtatc gtggcgggtc721 cgtccaccat cagtaagtat tcgtggtatg gatgcagtga tggaaacgct ggagcgtctt781 ggcctgcaac gctttattcg cacgaagcag gaaatcaaca aggaagcgat tttactggaa841 ccgaaagcgg tcgcaggcgt tgccggaatt acagttaaat caggcattga ggatttttct901 attattccat ttgaacagga agccggtatt agcggcagcg agactcccgg gacctcagag961 tccgctacac ccgaaagtag ctcagagact ggcccagtgg ctgtggaccc cacattgaga1021 cggcggatcg agccccatga gtttgaggta ttcttcgatc cgagagagct ccgcaaggag1081 acctgcctgc tttacgaaat taattggggg ggccggcact ccatttggcg acatacatca1141 cagaacacta acaagcacgt cgaagtcaac ttcatcgaga agttcacgac agaaagatat1201 ttctgtccga acacaaggtg cagcattacc tggtttctca gctggagccc atgcggcgaa1261 tgtagtaggg ccatcactga attcctgtca aggtatcccc acgtcactct gtttatttac1321 atcgcaaggc tgtaccacca cgctgacccc cgcaatcgac aaggcctgcg ggatttgatc1381 tcttcaggtg tgactatcca aattatgact gagcaggagt caggatactg ctggagaaac1441 tttgtgaatt atagcccgag taatgaagcc cactggccta ggtatcccca tctgtgggta1501 cgactgtacg ttcttgaact gtactgcatc atactgggcc tgcctccttg tctcaacatt1561 ctgagaagga agcagccaca gctgacattc tttaccatcg ctcttcagtc ttgtcattac1621 cagcgactgc ccccacacat tctctgggcc accgggttga aatctggtgg ttcttctggt1681 ggttctagcg gcagcgagac tcccgggacc tcagagtccg ccacacccga aagttctggt1741 ggttcttctg gtggttctgg gaaacggaac tacatcctgg ggcttgccat tgggataacc1801 agcgttggct acggaattat tgattatgag acacgcgatg tgattgacgc cggggttagg1861 ctgttcaaag aggccaacgt tgaaaacaac gagggaagac ggagtaagcg cggagcaaga1921 agactcaagc gcagacggag acatcggatt cagagggtga aaaagctgct cttcgattac1981 aatctcctga ccgatcatag tgagctgagc ggaatcaacc cctacgaggc gcgagtgaaa2041 gggctttccc agaagctgtc cgaagaggag ttctccgccg cgttgctgca cctggccaaa2101 cggagggggg ttcacaatgt aaacgaagtg gaggaggaca cgggcaatga acttagtacg2161 aaagaacaga tcagtaggaa ctctaaggct ctcgaagaga aatacgtcgc tgagttgcag2221 cttgagagac tgaaaaaaga cggcgaagta cgcggatcta ttaataggtt caagacttca2281 gattacgtaa aggaagccaa gcagctcctg aaagtacaga aagcgtacca tcagctcgat2341 cagagcttca tcgataccta catagatttg ctggagacac ggaggacata ctacgagggc2401 ccaggggaag gatctccttt tgggtggaag gacatcaagg aatggtacga gatgcttatg2461 ggacattgta catattttcc ggaggagctc aggagcgtca agtacgccta caatgccgac2521 ctgtacaatg ccctcaatga cctcaataac ctcgtgatta ccagggacga gaacgagaag2581 ctggagtact atgaaaagtt ccagattatc gagaatgtgt ttaagcagaa gaagaagccg2641 acacttaagc agattgcaaa ggaaatcctc gtgaatgagg aagatatcaa gggatacaga2701 gtgacaagta caggcaagcc cgagttcaca aatctgaagg tgtaccacga tattaaggac2761 ataaccgcac gaaaggagat aatcgaaaac gctgagctcc tcgatcagat cgcaaaaatt2821 cttaccatct accagtctag tgaggacatt caggaggaac tgactaatct gaacagtgag2881 ctcacccaag aggaaattga gcagatttca aacctgaaag gctacaccgg gacgcacaat2941 ctgagcctca aagcaatcaa cctcattctg gatgaacttt ggcacacaaa tgacaaccaa3001 attgccatat tcaaccgcct gaaactggtg ccaaaaaaag tggatctgtc acagcaaaag3061 gaaatcccta caaccttggt tgacgatttt attctgtccc ccgttgtcaa gcggagcttc3121 atccagtcaa tcaaggtgat caatgccatc attaaaaaat acggattgcc aaacgatata3181 attatcgagc ttgcacgaga gaagaactca aaggacgccc agaagatgat taacgaaatg3241 cagaagcgca accgccagac aaacgaacgc atagaggaaa ttataagaac aaccggcaaa3301 gagaatgcca agtatctgat cgagaaaatc aagctgcacg acatgcaaga aggcaagtgc&nb
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