- 商品货号:FH1705
- 规格:10μl质粒
- 原核抗性:卡那霉素Kan
- 筛选标记:遗传霉素G418
- 克隆菌株:大肠杆菌DH5α
- 质粒简介: pNTAP-B是一个哺乳细胞pCMV系列表达质粒。
- 质粒图谱1: png/1-1G221101542926.png
质粒序列: 点击查看序列LOCUS Exported 4531 bp ds-DNA circular SYN 19-DEC-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..4531/organism="synthetic DNA construct"/mol_type="other DNA"enhancer 67..370/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 371..574/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"promoter 620..638/label=T3 promoter/note="promoter for bacteriophage T3 RNA polymerase"CDS 682..759/codon_start=1/product="calmodulin-binding peptide"/label=CBP/note="derived from skeletal muscle myosin light chainkinase; binds calmodulin with nanomolar affinity in thepresence of calcium"/translation="KRRWKKNFIAVSAANRFKKISSSGAL"CDS 778..891/codon_start=1/product="streptavidin-binding peptide"/label=SBP/note="selected from a peptide library; binds streptavidinwith nanomolar affinity (Keefe et al., 2001)"/translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"misc_feature 914..987/label=MCSpromoter complement(1033..1051)/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"polyA_signal 1325..1446/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"rep_origin complement(1453..1908)/direction=LEFT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 1935..2039/gene="bla"/label=AmpR promoterpromoter 2041..2398/label=SV40 promoter/note="SV40 enhancer and early promoter"rep_origin 2249..2384/label=SV40 ori/note="SV40 origin of replication"CDS 2433..3227/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=NeoR/KanR/note="confers resistance to neomycin, kanamycin, and G418(Geneticin(R))"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"polyA_signal 3459..3506/label=HSV TK poly(A) signal/note="herpes simplex virus thymidine kinasepolyadenylation signal (Cole and Stacy, 1985)"rep_origin 3835..4423/direction=RIGHT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"ORIGIN1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc661 gcggtggcgg ccgccaccat gaagcgacga tggaaaaaga atttcatagc cgtctcagca721 gccaaccgct ttaagaaaat ctcatcctcc ggggcacttg gaagcggtag cggtaccatg781 gacgagaaga ccaccggctg gcggggcggc cacgtggtgg agggcctggc cggcgagctg841 gagcagctgc gggccaggct ggagcaccac cctcagggcc agcgggagcc ctccggcggc901 tgcaagctgg gctgcccggg cggatccccc gggctgcagg aattcgatat caagcttatc961 gataccgtcg acctcgaggg ggggcccggt accttaatta attaaggtac caggtaagtg1021 tacccaattc gccctatagt gagtcgtatt acaattcact cgatcgccct tcccaacagt1081 tgcgcagcct gaatggcgaa tggagatcca atttttaagt gtataatgtg ttaaactact1141 gattctaatt gtttgtgtat tttagattca cagtcccaag gctcatttca ggcccctcag1201 tcctcacagt ctgttcatga tcataatcag ccataccaca tttgtagagg ttttacttgc1261 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt1321 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt1381 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt1441 atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt1501 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag1561 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga1621 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg1681 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc1741 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg1801 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc1861 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt1921 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt1981 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagaatc2041 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg2101 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg2161 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc2221 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca2281 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc2341 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga2401 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag2461 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg2521 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca2581 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc2641 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg2701 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg2761 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta2821 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag2881 ccggtcttgt cgatcaggat gatctggacg aagaacatca ggggctcgcg ccagccgaac2941 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg3001 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg3061 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg3121 aagaacttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg3181 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg3241 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc3301 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct3361 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac3421 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa3481 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc3541 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc3601 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg3661 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta3721 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg3781 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga3841 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt3901 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag3961 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa4021 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag4081 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca4141 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac4201 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa4261 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc4321 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg4381 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc4441 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc4501 ccctgattct gtggataacc gtattaccgc c//
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