- 商品货号:FH1713
- 规格:10μl质粒
- 启动子:mU6
- 原核抗性:卡那霉素Kan
- 筛选标记:遗传霉素G418
- 克隆菌株:大肠杆菌DH5α
- 质粒简介: pmU6-gRNA是一个哺乳细胞gRNA表达质粒。
- 质粒图谱1: png/1-1G2201A019A0.png
质粒序列: 点击查看序列LOCUS Exported 3542 bp ds-DNA circular SYN 20-DEC-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..3542/organism="synthetic DNA construct"/mol_type="other DNA"rep_origin complement(3..458)/direction=LEFT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 670..984/label=mU6 promoter/note="RNA polymerase III promoter for mouse U6 snRNA (Daset al., 1988)"misc_RNA 1001..1076/label=gRNA scaffold/note="guide RNA scaffold for the Streptococcus pyogenesCRISPR/Cas9 system"rep_origin complement(1562..2150)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(2227..3021)/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=NeoR/KanR/note="confers resistance to neomycin, kanamycin, and G418(Geneticin(R))"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"promoter complement(3056..3413)/label=SV40 promoter/note="SV40 enhancer and early promoter"rep_origin 3070..3205/label=SV40 ori/note="SV40 origin of replication"promoter complement(3415..3519)/gene="bla"/label=AmpR promoterORIGIN1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacccg661 tctcagatag atccgacgcc gccatctcta ggcccgcgcc ggccccctcg cacagacttg721 tgggagaagc tcggctactc ccctgccccg gttaatttgc atataatatt tcctagtaac781 tatagaggct taatgtgcga taaaagacag ataatctgtt ctttttaata ctagctacat841 tttacatgat aggcttggat ttctataaga gatacaaata ctaaattatt attttaaaaa901 acagcacaaa aggaaactca ccctaactgt aaagtaattg tgtgttttga gactataaat961 atcccttgga gaaaagcctt gtttgtcttc gagaagacct gttttagagc tagaaatagc1021 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt1081 tttgtactga gacgccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg1141 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa1201 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga1261 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt1321 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct1381 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat1441 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga1501 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt1561 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt1621 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc1681 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa1741 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct1801 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta1861 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg1921 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc1981 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta2041 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg2101 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt2161 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg2221 tcatgatcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc2281 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat2341 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc2401 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg2461 ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc2521 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat2581 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg2641 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc2701 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc2761 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga2821 tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa2881 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt2941 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg3001 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc3061 aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg3121 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg3181 aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact3241 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc3301 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg3361 actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct caggactctt3421 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt3481 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc3541 ac//
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