• 商品货号：FH1783

• 规格：10μl质粒
• 复制子：pUC
• 原核抗性：氨苄青霉素Amp
• 筛选标记：嘌呤霉素Puro
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：              pGL4.22[luc2CP/Puro]是一个哺乳细胞单荧光素酶报告质粒。The pGL4.22[luc2CP/Puro] Vector encodes the luciferase reporter gene luc2CP (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector contains a mammalian selectable marker for puromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The vector is engineered with fewer consensus regulatory sequences for reduced background and a decreased risk of anomolous transcription and has a synthetic reporter gene, which has been codon optimized for mammalian expression. The pGL4.22[luc2CP/Puro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice. The luc2CP reporter gene contains two protein destabilization sequences, hCL1 and hPEST. The protein encoded by luc2CP responds more quickly and with greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.
• 质粒图谱1： png/1-1P106110035T9.png

质粒序列： 点击查看序列

LOCUS       Exported                5583 bp ds-DNA     circular SYN 06-JAN-2018

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..5583

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     regulatory      94..103

                     /regulatory_class="other"

                     /note="vertebrate consensus sequence for strong initiation 

                     of translation (Kozak, 1987)"

     CDS             100..1749

                     /codon_start=1

                     /product="firefly luciferase"

                     /label=luciferase

                     /note="synthetic luc2 version of the luciferase gene"

                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA

                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP

                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS

                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA

                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK

                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY

                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS

                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL

                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV

                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"

     CDS             1756..1803

                     /codon_start=1

                     /product="non-ORF yeast peptide conferring 

                     ubiquitin-dependent degradation"

                     /label=hCL1

                     /note="human codon-optimized"

                     /translation="ACKNWFSSLSHFVIHL"

     CDS             1807..1926

                     /codon_start=1

                     /product="PEST degradation sequence from mouse ornithine 

                     decarboxylase"

                     /label=hPEST

                     /note="human codon-optimized"

                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"

     polyA_signal    1975..2096

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     promoter        2290..2647

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      2498..2633

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             2678..3277

                     /codon_start=1

                     /product="puromycin N-acetyltransferase"

                     /label=PuroR

                     /note="confers resistance to puromycin"

                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER

                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA

                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS

                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"

     polyA_signal    3302..3350

                     /note="synthetic polyadenylation signal"

     rep_origin      complement(3677..4265)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(4465..5325)

                     /codon_start=1

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     polyA_signal    5430..5478

                     /note="synthetic polyadenylation signal"

     misc_feature    5492..5583

                     /label=pause site

                     /note="RNA polymerase II transcriptional pause signal from 

                     the human alpha-2 globin gene"

ORIGIN

        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc

       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt

      121 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac

      181 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc

      241 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg

      301 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag

      361 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac

      421 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc

      481 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa

      541 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc

      601 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac

      661 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc

      721 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc

      781 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc

      841 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc

      901 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg

      961 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc

     1021 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg

     1081 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc

     1141 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc

     1201 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc

     1261 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct

     1321 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac

     1381 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac

     1441 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc

     1501 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg

     1561 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca

     1621 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc

     1681 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag

     1741 atcgccgtga attctgcttg caagaactgg ttcagtagct taagccactt tgtgatccac

     1801 cttaacagcc acggcttccc tcccgaggtg gaggagcagg ccgccggcac cctgcccatg

     1861 agctgcgccc aggagagcgg catggataga caccctgctg cttgcgccag cgccaggatc

     1921 aacgtctaac tgcagtctag agtcggggcg gccggccgct tcgagcagac atgataagat

     1981 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg

     2041 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca

     2101 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa

     2161 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtttgcg tattgggcgc

     2221 tcttccgctg atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt

     2281 agctaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa

     2341 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac

     2401 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa

     2461 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag

     2521 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc

     2581 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt

     2641 ttgcaaaaag ctcgattctt ctgacactag cgccaccatg accgagtaca agcctaccgt

     2701 gcgcctggcc actcgcgatg atgtgccccg cgccgtccgc actctggccg ccgctttcgc

     2761 cgactacccc gctacccggc acaccgtgga ccccgaccgg cacatcgagc gtgtgacaga

     2821 gttgcaggag ctgttcctga cccgcgtcgg gctggacatc ggcaaggtgt gggtagccga

     2881 cgacggcgcg gccgtggccg tgtggactac ccccgagagc gttgaggccg gcgccgtgtt

     2941 cgccgagatc ggcccccgaa tggccgagct gagcggcagc cgcctggccg cccagcagca

     3001 aatggagggc ctgcttgccc cccatcgtcc caaggagcct gcctggtttc tggccactgt

     3061 aggagtgagc cccgaccacc agggcaaggg cttgggcagc gccgtcgtgt tgcccggcgt

     3121 agaggccgcc gaacgcgccg gtgtgcccgc ctttctcgaa acaagcgcac caagaaacct

     3181 tccattctac gagcgcctgg gcttcaccgt gaccgccgat gtcgaggtgc ccgagggacc

     3241 taggacctgg tgtatgacac gaaaacctgg cgcctaatga tctagaaccg gtcatggccg

     3301 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg ttcgaactag

     3361 atgctgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg

     3421 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca

     3481 ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc

     3541 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg

     3601 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg

     3661 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac

     3721 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg

     3781 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct

     3841 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg

     3901 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct

     3961 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac

     4021 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt

     4081 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc

     4141 tgctgaagcc agttaccttc ggaaaaagag