- 商品货号:FH1881
- 规格:10μl质粒
- 原核抗性:氨苄青霉素Amp
- 筛选标记:嘌呤霉素Puro
- 克隆菌株:大肠杆菌Stbl3
- 质粒简介: Retroviral vector that expresses wild type Flag-tagged human USP7
- 质粒图谱1: png/1-1P224152021216.png
质粒序列: 点击查看序列LOCUS Exported 10491 bp ds-DNA circular SYN 24-FEB-2018FEATURES Location/Qualifierssource 1..10491/organism="synthetic DNA construct"/mol_type="other DNA"promoter 309..512/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"LTR 513..688/label=5' LTR (truncated)/note="truncated long terminal repeat from Moloney murinesarcoma virus"misc_feature 751..950/label=MMLV Psi/note="packaging signal of Moloney murine leukemia virus(MMLV)"CDS 1151..1567/label=gag (truncated)/note="truncated Moloney murine leukemia virus (MMLV) gaggene lacking the start codon"enhancer 1604..1907/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 1908..2111/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"CDS 2265..2288/codon_start=1/product="FLAG(R) epitope tag, followed by an enterokinasecleavage site"/label=FLAG/translation="DYKDDDDK"CDS 2289..5603/codon_start=1/label=USP7/translation="TGMNHQQQQQQQKAGEQQLSEPEDMEMEAGDTDDPPRITQNPVINGNVALSDGHNTAEEDMEDDTSWRSEATFQFTVERFSRLSESVLSPPCFVRNLPWKIMVMPRFYPDRPHQKSVGFFLQCNAESDSTSWSCHAQAVLKIINYRDDEKSFSRRISHLFFHKENDWGFSNFMAWSEVTDPEKGFIDDDKVTFEVFVQADAPHGVAWDSKKHTGYVGLKNQGATCYMNSLLQTLFFTNQLRKAVYMMPTEGDDSSKSVPLALQRVFYELQHSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNVENKMKGTCVEGTIPKLFRGKMVSYIQCKEVDYRSDRREDYYDIQLSIKGKKNIFESFVDYVAVEQLDGDNKYDAGEHGLQEAEKGVKFLTLPPVLHLQLMRFMYDPQTDQNIKINDRFEFPEQLPLDEFLQKTDPKDPANYILHAVLVHSGDNHGGHYVVYLNPKGDGKWCKFDDDVVSRCTKEEAIEHNYGGHDDDLSVRHCTNAYMLVYIRESKLSEVLQAVTDHDIPQQLVERLQEEKRIEAQKRKERQEAHLYMQVQIVAEDQFCGHQGNDMYDEEKVKYTVFKVLKNSSLAEFVQSLSQTMGFPQDQIRLWPMQARSNGTKRPAMLDNEADGNKTMIELSDNENPWTIFLETVDPELAASGATLPKFDKDHDVMLFLKMYDPKTRSLNYCGHIYTPISCKIRDLLPVMCDRAGFIQDTSLILYEEVKPNLTERIQDYDVSLDKALDELMDGDIIVFQKDDPENDNSELPTAKEYFRDLYHRVDVIFCDKTIPNDPGFVVTLSNRMNYFQVAKTVAQRLNTDPMLLQFFKSQGYRDGPGNPLRHNYEGTLRDLLQFFKPRQPKKLYYQQLKMKITDFENRRSFKCIWLNSQFREEEITLYPDKHGCVRDLLEECKKAVELGEKASGKLRLLEIVSYKIIGVHQEDELLECLSPATSRTFRIEEIPLDQVDIDKENEMLVTVAHFHKEVFGTFGIPFLLRIHQGEHFREVMKRIQSLLDIQEKEFEKFKFAIVMTGRHQYINEDEYEVNLKDFEPQPGNMSHPRPWLGLDHFNKAPKRSRYTYLEKAIKIHN"misc_feature 5619..6191/label=IRES/note="internal ribosome entry site (IRES) of theencephalomyocarditis virus (EMCV)"CDS 6224..6823/codon_start=1/gene="pac from Streptomyces"/product="puromycin N-acetyltransferase"/label=PuroR/note="confers resistance to puromycin"/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"LTR 7197..7622/label=3' LTR (Delta-U3)/note="self-inactivating 3' long terminal repeat (LTR) fromMoloney murine leukemia virus"promoter 7888..8217/label=SV40 promoter/note="SV40 enhancer and early promoter"rep_origin complement(8510..9098)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(9269..10129)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(10130..10234)/gene="bla"/label=AmpR promoterenhancer 10420..308/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"ORIGIN1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt1921 ggcagtacat caatgggcgt gaatagcggt ttgactcacg gggatttcca agtctccacc1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa2221 cgctgcagga attgatccgc ggccgcgaca ccatggcaca attggattac aaggatgacg2281 atgacaagac cggtatgaac caccagcagc agcagcagca gcagaaagcg ggcgagcagc2341 agttgagcga gcccgaggac atggagatgg aagcgggaga tacagatgac ccaccaagaa2401 ttactcagaa ccctgtgatc aatgggaatg tggccctgag tgatggacac aacaccgcgg2461 aggaggacat ggaggatgac accagttggc gctccgaggc aacctttcag ttcactgtgg2521 agcgcttcag cagactgagt gagtcggtcc ttagccctcc gtgttttgtg cgaaatctgc2581 catggaagat tatggtgatg ccacgctttt atccagacag accacaccaa aaaagcgtag2641 gattctttct ccagtgcaat gctgaatctg attccacgtc atggtcttgc catgcacaag2701 cagtgctgaa gataataaat tacagagatg atgaaaagtc gttcagtcgt cgtattagtc2761 atttgttctt ccataaagaa aatgattggg gattttccaa ttttatggcc tggagtgaag2821 tgaccgatcc tgagaaagga tttatagatg atgacaaagt tacctttgaa gtctttgtac
服务时间:24小时在线客服
客服热线:400-000-7085
丰晖生物
中国优质生物资源平台