×
![]()
- 规格：10μl质粒
- 原核抗性：氨苄青霉素Amp
- 筛选标记：潮霉素Hyg，绿色荧光蛋白EGFP
- 克隆菌株：大肠杆菌DH5α
- 质粒简介： pCT74是一个真菌表达质粒，可用原生质体法转化进各种真菌。
- 质粒图谱1： jpg/8-1P12G50921118.jpg
质粒序列： 点击查看序列LOCUS Exported 5790 bp ds-DNA circular SYN 25-JAN-2018DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..5790/organism="synthetic DNA construct"/mol_type="other DNA"primer_bind 19..35/label=SK primer/note="common sequencing primer, one of multiple similarvariants"terminator 78..330/label=NOS terminator/note="nopaline synthase terminator and poly(A) signal"CDS complement(349..1068)/codon_start=1/product="enhanced GFP"/label=EGFP/note="mammalian codon-optimized"/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK"CDS 1855..2880/codon_start=1/gene="aph(4)-Ia"/product="aminoglycoside phosphotransferase from E. coli"/label=HygR/note="confers resistance to hygromycin"/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"promoter complement(2947..2965)/label=T3 promoter/note="promoter for bacteriophage T3 RNA polymerase"primer_bind complement(2986..3002)/label=M13 rev/note="common sequencing primer, one of multiple similarvariants"protein_bind 3010..3026/label=lac operator/bound_moiety="lac repressor encoded by lacI"/note="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can berelieved by adding lactose orisopropyl-beta-D-thiogalactopyranoside (IPTG)."promoter complement(3034..3064)/label=lac promoter/note="promoter for the E. coli lac operon"protein_bind 3079..3100/label=CAP binding site/bound_moiety="E. coli catabolite activator protein"/note="CAP binding activates transcription in the presenceof cAMP."rep_origin complement(3388..3976)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(4147..5007)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(5008..5112)/gene="bla"/label=AmpR promoterrep_origin complement(5138..5593)/direction=LEFT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"primer_bind 5735..5751/label=M13 fwd/note="common sequencing primer, one of multiple similarvariants"promoter 5758..5776/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"ORIGIN1 caccgcggtg gcggccgctc tagaactagt ggatcccccg ggctgcagga attctcatgt61 ttgacagctt atcatcggat ctagtaacat agatgacacc gcgcgcgata atttatccta121 gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat181 cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac241 gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa301 gaaactttat tgccaaatgt ttgaacgatc tgcagccggg cggccgcttt acttgtacag361 ctcgtccatg ccgtgagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc421 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg481 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac541 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt601 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat661 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc721 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg781 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc841 ggggtagcgg ctgaagcact gcacgccgta ggtgaaggtg gtcacgaggg tgggccaggg901 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc961 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac1021 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg cctatattca1081 ttcattgtca gctatcgctg gcaacaagat ataagcgtgt acttacgatt ctaggaccct1141 gcgaatagag ttggagttgg ataccgatgg acgattgtta gataggacgg gatgctgata1201 gtcgaggtaa gttgttgagg gtggtggttt tatcaaggac ggacacctta gccctttata1261 tacacagaat cttattgcta ggtagccgga cggtgcggat tccaggtatc agtaggccct1321 ctgaatgtcc atgcaatgat gcccaacaat cgagacgatc ctacttcgga accgactcga1381 tcctcgtcgg gcctacatgt atgagaccat gtgcacctcc gaagcatcct tacggagagc1441 ggaattgcgc gtacagaact cctccatgca ttccaatcga taccgtcgac gttaactgat1501 attgaaggag cattttttgg gcttggctgg agctagtgga ggtcaacaat gaatgcctat1561 tttggtttag tcgtccaggc ggtgagcaca aaatttgtgt cgtttgacaa gatggttcat1621 ttaggcaact ggtcagatca gccccacttg tagcagtagc ggcggcgctc gaagtgtgac1681 tcttattagc agacaggaac gaggacatta ttatcatctg ctgcttggtg cacgataact1741 tggtgcgttt gtcaagcaag gtaagtggac gacccggtca taccttctta agttcgccct1801 tcctcccttt atttcagatt caatctgact tacctattct acccaagcat ccaaatgaaa1861 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc1921 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga1981 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat2041 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggag2101 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac2161 ctgcctgaaa ccgaactgcc cgctgttctc cagccggtcg cggaggccat ggatgcgatc2221 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt2281 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg2341 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg2401 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc atgcggattt cggctccaac2461 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc2521 ggggattccc aatacgaggt cgccaacatc ctcttctgga ggccgtggtt ggcttgtatg2581 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcgcctc2641 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat2701 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg2761 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta2821 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag2881 agtagatgcc gaccgggaac cagttaacgt cgacctcgag ggggggcccg gtaccagctt2941 ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc3001 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg3061 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc3121 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg3181 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc3241 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac3301 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa3361 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca3421 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc3481 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata3541 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta3601 tctcag参考文献
Succinate Dehydrogenase Inhibitors as Triggers of Azole Resistance in Aspergillus fumigatus: The Role of sdh1 and Efflux Pathways(2024-12-18)IF: 0.00
- 作者：Heng Zhang, Zhangling Zhu, Xiao Gong, Wenxu Cheng, Weizu Liao
- 单位：长江大学荆州市附属医院
- 期刊： Research Square
- DOI： 10.21203/rs.3.rs-5441096/v1
产品：
