- 商品货号:FH1153
- 规格:10μl质粒
- 启动子:CMV promoter
- 质粒大小:9237bp
- 质粒标签:C-FLAG
- 原核抗性:氨苄青霉素Amp
- 筛选标记:新霉素Neo/遗传霉素G418
- 克隆菌株:大肠杆菌Stbl3
- 质粒简介: pLEGFP-WT-STAT3 是一个过表达人源STAT3基因的逆病毒质粒,STAT3基因的C端融合了FLAG标签,但没有融合EGFP标签。
- 质粒图谱1: png/1-1G011093103296.png
质粒序列: 点击查看序列LOCUS Exported 9237 bp ds-DNA circular SYN 11-OCT-2017DEFINITION synthetic circular DNAACCESSION .VERSION .KEYWORDS .SOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 9237)AUTHORS .TITLE Direct Submissionhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..9237/organism="synthetic DNA construct"/mol_type="other DNA"enhancer 1..304/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 305..508/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"misc_feature 572..2881/label=STAT3CDS 2882..2905/codon_start=1/product="FLAG(R) epitope tag, followed by an enterokinasecleavage site"/label=FLAG/translation="DYKDDDDK"regulatory 2960..2969/regulatory_class="other"/note="vertebrate consensus sequence for strong initiationof translation (Kozak, 1987)"CDS 2966..3685/codon_start=1/product="enhanced GFP"/label=EGFP/note="mammalian codon-optimized"/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"LTR 3763..4356/note="long terminal repeat from Moloney murine leukemiavirus"misc_feature 4567..4707/label=bom/note="basis of mobility region from pBR322"rep_origin complement(4893..5481)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(5652..6512)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(6513..6617)/gene="bla"/label=AmpR promoterLTR 7275..7450/label=5' LTR (truncated)/note="truncated long terminal repeat from Moloney murinesarcoma virus"misc_feature 7513..7712/label=MMLV Psi/note="packaging signal of Moloney murine leukemia virus(MMLV)"CDS 7913..8329/label=gag (truncated)/note="truncated Moloney murine leukemia virus (MMLV) gaggene lacking the start codon"CDS 8373..9167/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=Neo/note="confers resistance to neomycin, kanamycin, and G418(Geneticin(R))"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"ORIGIN1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta541 ccggactcag atctcgagct caagcttcag gatggcccaa tggaatcagc tacagcagct601 tgacacacgg tacctggagc agctccatca gctctacagt gacagcttcc caatggagct661 gcggcagttt ctggcccctt ggattgagag tcaagattgg gcatatgcgg ccagcaaaga721 atcacatgcc actttggtgt ttcataatct cctgggagag attgaccagc agtatagccg781 cttcctgcaa gagtcgaatg ttctctatca gcacaatcta cgaagaatca agcagtttct841 tcagagcagg tatcttgaga agccaatgga gattgcccgg attgtggccc ggtgcctgtg901 ggaagaatca cgccttctac agactgcagc cactgcggcc cagcaagggg gccaggccaa961 ccaccccaca gcagccgtgg tgacggagaa gcagcagatg ctggagcagc accttcagga1021 tgtccggaag agagtgcagg atctagaaca gaaaatgaaa gtggtagaga atctccagga1081 tgactttgat ttcaactata aaaccctcaa gagtcaagga gacatgcaag atctgaatgg1141 aaacaaccag tcagtgacca ggcagaagat gcagcagctg gaacagatgc tcactgcgct1201 ggaccagatg cggagaagca tcgtgagtga gctggcgggg cttttgtcag cgatggagta1261 cgtgcagaaa actctcacgg acgaggagct ggctgactgg aagaggcggc aacagattgc1321 ctgcattgga ggcccgccca acatctgcct agatcggcta gaaaactgga taacgtcatt1381 agcagaatct caacttcaga cccgtcaaca aattaagaaa ctggaggagt tgcagcaaaa1441 agtttcctac aaaggggacc ccattgtaca gcaccggccg atgctggagg agagaatcgt1501 ggagctgttt agaaacttaa tgaaaagtgc ctttgtggtg gagcggcagc cctgcatgcc1561 catgcatcct gaccggcccc tcgtcatcaa gaccggcgtc cagttcacta ctaaagtcag1621 gttgctggtc aaattccctg agttgaatta tcagcttaaa attaaagtgt gcattgacaa1681 agactctggg gacgttgcag ctctcagagg atcccggaaa tttaacattc tgggcacaaa1741 cacaaaagtg atgaacatgg aagaatccaa caacggcagc ctctctgcag aattcaaaca1801 cttgaccctg agggagcaga gatgtgggaa tgggggccga gccaattgtg atgcttccct1861 gattgtgact gaggagctgc acctgatcac ctttgagacc gaggtgtatc accaaggcct1921 caagattgac ctagagaccc actccttgcc agttgtggtg atctccaaca tctgtcagat1981 gccaaatgcc tgggcgtcca tcctgtggta caacatgctg accaacaatc ccaagaatgt2041 aaactttttt accaagcccc caattggaac ctgggatcaa gtggccgagg tcctgagctg2101 gcagttctcc tccaccacca agcgaggact gagcatcgag cagctgacta cactggcaga2161 gaaactcttg ggacctggtg tgaattattc agggtgtcag atcacatggg ctaaattttg2221 caaagaaaac atggctggca agggcttctc cttctgggtc tggctggaca atatcattga2281 ccttgtgaaa aagtacatcc tggccctttg gaacgaaggg tacatcatgg gctttatcag2341 taaggagcgg gagcgggcca tcttgagcac taagcctcca ggcaccttcc tgctaagatt2401 cagtgaaagc agcaaagaag gaggcgtcac tttcacttgg gtggagaagg acatcagcgg2461 taagacccag atccagtccg tggaaccata cacaaagcag cagctgaaca acatgtcatt2521 tgctgaaatc atcatgggct ataagatcat ggatgctacc aatatcctgg tgtctccact2581 ggtctatctc tatcctgaca ttcccaagga ggaggcattc ggaaagtatt gtcggccaga2641 gagccaggag catcctgaag ctgacccagg tagcgctgcc ccatacctga agaccaagtt2701 tatctgtgtg acaccaacga cctgcagcaa taccattgac ctgccgatgt ccccccgcac2761 tttagattca ttgatgcagt ttggaaataa tggtgaaggt gctgaaccct cagcaggagg2821 gcagtttgag tccctcacct ttgacatgga gttgacctcg gagtgcgcta cctcccccat2881 ggactacaag gacgacgatg acaagtgaaa gcttcgaatt ctgcagtcga cggtaccgcg2941 ggcccgggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt3001 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg3061 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg3121 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt3181 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg3241 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga3301 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa3361 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta3421 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat3481 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg3541 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
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