• 商品货号：FH1768

• 规格：10μl质粒
• 启动子：CMV
• 复制子：pUC
• 终止子：bGH
• 原核抗性：卡那霉素Kan
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：           pEGFP-KIF3b(NM_004798.3)是一个人源基因哺乳过表达质粒。
• 质粒图谱1： png/1-1G222111940593.png

质粒序列： 点击查看序列

LOCUS       Exported                6957 bp ds-DNA     circular SYN 05-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..6957

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        61..364

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        365..568

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     regulatory      607..616

                     /regulatory_class="other"

                     /note="vertebrate consensus sequence for strong initiation 

                     of translation (Kozak, 1987)"

     CDS             613..1329

                     /codon_start=1

                     /product="enhanced GFP"

                     /label=EGFP

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     CDS             1351..3594

                     /codon_start=1

                     /label=KIF3B

                     /translation="MSKLKSSESVRVVVRCRPMNGKEKAASYDKVVDVDVKLGQVSVKN

                     PKGTAHEMPKTFTFDAVYDWNAKQFELYDETFRPLVDSVLQGFNGTIFAYGQTGTGKTY

                     TMEGIRGDPEKRGVIPNSFDHIFTHISRSQNQQYLVRASYLEIYQEEIRDLLSKDQTKR

                     LELKERPDTGVYVKDLSSFVTKSVKEIEHVMNVGNQNRSVGATNMNEHSSRSHAIFVIT

                     IECSEVGLDGENHIRVGKLNLVDLAGSERQAKTGAQGERLKEATKINLSLSALGNVISA

                     LVDGKSTHIPYRDSKLTRLLQDSLGGNAKTVMVANVGPASYNVEETLTTLRYANRAKNI

                     KNKPRVNEDPKDALLREFQEEIARLKAQLEKRSIGRRKRREKRREGGGSGGGGEEEEEE

                     GEEGEEEGDDKDDYWREQQEKLEIEKRAIVEDHSLVAEEKMRLLKEKEKKMEDLRREKD

                     AAEMLGAKIKAMESKLLVGGKNIVDHTNEQQKILEQKRQEIAEQKRREREIQQQMESRD

                     EETLELKETYSSLQQEVDIKTKKLKKLFSKLQAVKAEIHDLQEEHIKERQELEQTQNEL

                     TRELKLKHLIIENFIPLEEKSKIMNRAFFDEEEDHWKLHPITRLENQQMMKRPVSAVGY

                     KRPLSQHARMSMMIRPEARYRAENIVLLELDMPSRTTRDYEGPAIAPKVQAALDAALQD

                     EDEIQVDASSFESTANKKSKARPKSGRKSGSSSSSSGTPASQLYPQSRGLVPK"

     polyA_signal    3745..3866

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(3873..4328)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        4355..4459

                     /gene="bla"

                     /label=AmpR promoter

     promoter        4461..4818

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      4669..4804

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             4853..5647

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    5879..5926

                     /label=HSV TK poly(A) signal

                     /note="herpes simplex virus thymidine kinase 

                     polyadenylation signal (Cole and Stacy, 1985)"

     rep_origin      6255..6843

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg

       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt

      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca

      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc

      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta

      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac

      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg

      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg

      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt

      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta

      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg

      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc

      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg

      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc

      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag

      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag

      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac

     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac

     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc

     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg

     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc

     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag

     1321 ctgtacaagt ccggactcag atctcgagct atgtcaaagt tgaaaagctc agagtcagtc

     1381 agggtggtgg ttcgctgtcg gcccatgaat ggcaaggaaa aggctgcttc gtatgacaaa

     1441 gtggtggatg tggatgttaa gctggggcag gtgtctgtga agaaccccaa agggacggcc

     1501 catgaaatgc ccaagacctt cacctttgat gccgtctatg actggaatgc caagcagttt

     1561 gaactgtacg atgagacgtt ccgaccactt gttgactctg tcctgcaagg tttcaatgga

     1621 accatttttg cctatggaca aactgggaca ggaaaaacct acaccatgga aggaatccgt

     1681 ggtgaccctg aaaaaagagg agtcattcct aactcatttg accatatctt cacccacatc

     1741 tctcgatccc agaatcaaca atacctggtc agggcttctt acttagagat ctaccaggag

     1801 gagatccgag atttgctctc aaaggatcag accaaaaggc ttgagctcaa agagaggcct

     1861 gacacaggag tgtatgtgaa agacctgtct tcctttgtca ccaagagtgt gaaggagata

     1921 gagcatgtga tgaatgtggg gaaccagaac cgttctgtcg gtgctaccaa catgaacgag

     1981 cacagctcgc gttctcatgc aattttcgtt atcactattg agtgcagcga ggtgggcctc

     2041 gatggtgaaa accacatccg tgtaggaaaa ttgaaccttg tagatcttgc tggcagcgaa

     2101 cggcaagcca agaccggcgc acaaggggag agattaaaag aagctaccaa gatcaacctc

     2161 tccctttccg ctttgggtaa tgtcatctct gctctagtgg acggcaaaag cactcacatt

     2221 ccatatcggg actcaaagct taccaggctc ctccaagatt cccttggtgg caatgccaag

     2281 actgtgatgg tggccaacgt ggggcctgcc tcttacaacg tagaagagac tctgaccact

     2341 ctgcgatatg ccaaccgtgc caaaaacatt aagaacaaac caagggtcaa tgaggacccc

     2401 aaggatgccc tccttcgaga attccaggaa gagattgctc ggctcaaggc ccagctggaa

     2461 aaacggtcca ttggtaggag gaagaggcga gagaagcgga gggaaggtgg tggcagtggt

     2521 gggggtgggg aagaggagga ggaggaggga gaagagggtg aggaggaagg ggatgataag

     2581 gatgattact ggcgggaaca gcaagaaaaa ctggagattg agaagcgggc cattgtagag

     2641 gatcacagct tggttgcaga ggagaagatg aggctgctga aggagaaaga gaaaaagatg

     2701 gaggacctgc ggcgggagaa ggatgctgcc gagatgctgg gcgccaagat caaggccatg

     2761 gagagtaagt tgcttgttgg aggaaaaaat atagtagatc atacgaatga acagcagaaa

     2821 atcctggagc agaaacgaca ggaaattgca gagcagaaac gtcgagaaag agaaatccag

     2881 caacagatgg aaagtcgaga tgaggagacc ttggaactta aagagacata cagctcattg

     2941 cagcaagagg tggacatcaa gaccaaaaaa ctcaaaaagc tcttctccaa gcttcaggca

     3001 gtgaaggctg agatccatga cctccaagaa gaacacatca aggagcgcca agagctagag

     3061 cagactcaga atgagctcac cagggagctg aaactcaagc atcttattat agaaaacttt

     3121 atccctctgg aagaaaaaag taaaattatg aatagagcct tctttgatga agaggaagat

     3181 cattggaaac tacatcctat aaccagactg gagaaccagc agatgatgaa gcggccagtc

     3241 tcagccgtgg gatataagag accattgagc cagcacgcaa gaatgtccat gatgattcgt

     3301 ccagaggccc gatatagggc agaaaacatt gtgctgttag agctggacat gcccagccgg

     3361 accaccagag actatgaggg tccagccatt gcccccaagg tccaggctgc attggatgcg

     3421 gctctgcagg atgaagatga gatacaggtg gatgcatcat catttgaaag cactgcaaat

     3481 aagaaatcca aggccaggcc taaaagtgga aggaagtcgg gatcctcctc ctcttcctca

     3541 ggaacccctg catctcagct ttatccacag tctcgggggc tggttccaaa gtaagtcgac

     3601 ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag ccataccaca

     3661 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat

     3721 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa

     3781 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt

     3841 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa

     3901 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa

     3961 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa

     4021 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca

     4081 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg

     4141 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc

     4201 ggcgaacgtg gcgagaaa