- Source: Chinese Hamster Ovary cell line
- Description: Human Noggin (Gln28-Cys232) Accession # Q13253
- Predicted molecular mass: 23 kDa
- Purity: ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
- Endotoxin: ≤10 EU/mg by the LAL method
- Activity: Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 2.0-3.0 ng/ml of Noggin.
- Formulation: Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
- Appearance: White powder, Colorless clear liquid after reconstitution
- Reconstitution: It is recommended to redissolve in sterile deionized water.
- Shipping: The product is shipped with blue ice.
- Storage & Stability: 36 months at -20°C to -80°C in lyophilized state 7-10 days at 2°C to 8°C under sterile conditions after reconstitution 6 months at -20°C to -80°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Data