• Source： Chinese Hamster Ovary cell line
• Description： Human R-Spondin1 (Ser21-Ala263) Accession # Q2MKA7
• Predicted molecular mass： 26.7kDa
• Purity： ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
• Endotoxin： ≤10 EU/mg by the LAL method
• Activity： R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells by testing alkaline phosphatase production. The expected ED50 is 1.0-3.0 μg/ml.
• Formulation： Lyophilized from a 0.2 μm filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
• Appearance： White powder, Colorless clear liquid after reconstitution
• Reconstitution： It is recommended to redissolve in sterile deionized water.
• Shipping： The product is shipped with blue ice
• Storage & Stability： 36 months at -20°C to -80°C in lyophilized state 6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution