质粒宿主: 植物 质粒用途: 基因编辑 片段类型: CRISPR 片段物种: 空载体 原核抗性: Kan 筛选标记: Bar 启动子: OsU3,UBI 复制子: pVS1 感受态: Stbl3 温度: 37度 参考序列: 点击查看序列gtttacccgccaatatatcctgtcaaacactgatagtttaaactgaaggcgggaaacgacaatctgatccaagctcaagctgctctagcattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagcttcgacttgccttccgcacaatacatcatttcttcttagctttttttcttcttcttcgttcatacagtttttttttgtttatcagcttacattttcttgaaccgtagctttcgttttcttctttttaactttccattcggagtttttgtatcttgtttcatagtttgtcccaggattagaatgattaggcatcgaaccttcaagaatttgattgaataaaacatcttcattcttaagatatgaagataatcttcaaaaggcccctgggaatctgaaagaagagaagcaggcccatttatatgggaaagaacaatagtatttcttatataggcccatttaagttgaaaacaatcttcaaaagtcccacatcgcttagataagaaaacgaagctgagtttatatacagctagagtcgaagtagtgattgggagaccaacccagtggacataagcctgttcggttcgtaagctgtaatgcaagtagcgtatgcgctcacgcaactggtccagaaccttgaccgaacgcagcggtggtaacggcgcagtggcggttttcatggcttgttatgactgtttttttggggtacagtctatgcctcgggcatccaagcagcaagcgcgttacgccgtgggtcgatgtttgatgttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaaacatcatgggggaagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattctccgcgctgtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagctaagcgcgaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcgttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttgaggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaatgtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaaggatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccgtcatacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgcagatcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaataatgtctagctagaaattcgttcaagccgacgccgcttcgcggcgcggcttaactcaagcgttagatgcactaagcacataattgctcacagccaaactatcaggtcaagtctgcttttattatttttaagcgtgcataataagccggtctcggttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttttgcaaaattttccagatcgatttcttcttcctctgttcttcggcgttcaatttctggggttttctcttcgttttctgtaactgaaacctaaaatttgacctaaaaaaaatctcaaataatatgattcagtggttttgtacttttcagttagttgagttttgcagttccgatgagataaaccaataagcttgcatgcctgcaggtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagatacagtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcggattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctcctacaaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgataacatggtggagcacgacacacttgtctactccaaaaatatcaaagatacagtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcggattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctcctacaaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagaggacctcgacctcaacacaacatatacaaaacaaacgaatctcaagcaatcaagcattctacttctattgcagcaatttaaatcatttcttttaaagcaaaagcaattttctgaaaattttcaccatttacgaacgatactcgagtaatctagatgtcttccgagacaggaccggttgccgtcgaccctactcttagaaggcgcattgagccacacgagttcgaggtgttcttcgatccgagagagctgaggaaggagacttgcctcctttacgagatcaattggggcggaaggcactctatttggcgccatacctcccagaacacaaataagcatgtggaggttaatttcatcgagaagttcactaccgagaggtacttctgcccaaacacacgctgcagcatcacttggttccttagctggtcaccgtgcggagagtgctctcgcgccattacagagttcctgtccagatacccgcacgttactcttttcatctacattgccagactgtaccaccatgcggatcctcgcaacagacagggtcttagggacctgatcagctcaggcgtcaccatccagattatgacagagcaggagtctggatactgctggcgcaacttcgtgaattactctccttccaatgaggctcactggccaagatacccgcatctgtgggtcaggctctacgtgctcgagctttactgcatcattcttggtctgcctccatgcctcaacatccttagaaggaagcagccacagctcacattcttcactattgcccttcagtcttgccactaccagaggcttccgcctcatattctgtgggcgactggcctcaagagcggctcagagactccgggaacatctgagtccgctactcctgagtctgacaagaagtactccatcggactcgccattggtactaactccgttggatgggcggtcatcaccgatgagtacaaggtgcctagcaagaagttcaaggttcttggtaacacagacagacactcaatcaagaagaatctgattggtgctctgctcttcgattctggagagactgccgaggctaccaggctcaagagaaccgcccgcagaaggtacacacgcagaaagaataggatctgctaccttcaggagattttctctaacgagatggctaaggttgatgacagcttcttccatcgccttgaggagtcattcctggtcgaggaggacaagaagcacgagagacatcctatcttcggtaacattgtcgatgaggtggcctaccacgagaagtacccaactatctaccatcttaggaagaagctggtggatagcaccgacaaggcggatctccgccttatctacctggctctcgcccacatgattaagttcagaggccatttcctcatcgagggcgatctcaacccagataattcagacgtcgataagctcttcatccagcttgtgcagacatacaatcagcttttcgaggagaacccgattaatgcgagcggtgttgatgcgaaggctatcctgtcagctagactcagcaagtcaaggcgcctggagaacctcatcgcccagctgccaggcgagaagaagaacggtcttttcggcaatctgattgcgctttctctgggactcaccccgaacttcaagtccaatttcgacctggctgaggatgccaagctccagctgtctaaggatacatacgatgacgatctcgacaaccttctggctcagatcggcgaccagtacgccgatctcttccttgctgccaagaatcttagcgatgccatcctcctttcagacattctgagagttaacactgagattaccaaggctccgctgtctgcctccatgatcaagagatacgatgagcaccatcaggacctcactctgctcaaggcgctggtccgccagcagctccctgagaagtacaaggagatcttcttcgaccagtctaagaacggctacgcgggttacattgatggtggcgctagccaggaggagttctacaagttcatcaagccaattctggagaagatggatggcactgaggagcttctggtcaagctca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