规格：20μl质粒

质粒宿主：  植物

质粒用途：  基因编辑

片段类型：  CRISPR

片段物种：  

原核抗性：  Kan

筛选标记：  Bar

荧光标记：  

启动子:  35S

复制子:  pVS1

拷贝数:  

感受态:  Stbl3

温度:  37度

背景载体:  

正向引物:  

反向引物:  

诱导方式:

质粒序列： 点击查看序列

cacatacaaatggacgaacggataaaccttttcacgcccttttaaatatccgttattctaataaacgctcttttctcttaggtttacccgccaatatatcctgtcaaacactgatagtttaaactgaaggcgggaaacgacaatctgatccaagctcaagctgctctagcattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagcttgcatgcctgcaGGTCAACATGGTGGAGCACGACACACTTGTCTACTCCAAAAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCAATTGAGACTTTTCAACAAAGGGTAATATCCGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTTATTGTGAAGATAGTGGAAAAGGAAGGTGGCTCCTACAAATGCCATCATTGCGATAAAGGAAAGGCCATCGTTGAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATAACATGGTGGAGCACGACACACTTGTCTACTCCAAAAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCAATTGAGACTTTTCAACAAAGGGTAATATCCGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTTATTGTGAAGATAGTGGAAAAGGAAGGTGGCTCCTACAAATGCCATCATTGCGATAAAGGAAAGGCCATCGTTGAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACCTCGACTCTAGAGGATCCCCGGGTACCGGGCCCCCCCTCGAGGcgcgccaccaggtcgacatggataagaagtactccatcggactggatatcggaactaactccgtgggatgggctgtgatcactgatgagtacaaggtgccatccaagaagttcaaggtgctgggaaacactgatagacactccatcaagaagaacctgatcggagctctgctgttcgattccggagagactgctgaggctactagactgaagagaactgctagaagaagatacactagaagaaagaacagaatctgctacctgcaagagatcttctccaacgagatggctaaggtggatgattccttcttccacagactggaggagtccttcctggtggaggaggataagaagcacgagagacacccaatcttcggaaacatcgtggatgaggtggcttaccacgagaagtacccaactatctaccacctgagaaagaagctggtggattccactgataaggctgatctgagactgatctacctggctctggctcacatgatcaagttcagaggacacttcctgatcgagggagatctgaacccagataactccgatgtggataagctgttcatccaactggtgcaaacttacaaccaactgttcgaggagaacccaatcaacgcttccggagtggatgctaaggctatcctgtccgctagactgtccaagtccagaagactggagaacctgatcgctcaactgccaggagagaagaagaacggactgttcggaaacctgatcgctctgtccctgggactgactccaaacttcaagtccaacttcgatctggctgaggatgctaagctgcaactgtccaaggatacttacgatgatgatctggataacctgctggctcaaatcggagatcaatacgctgatctgttcctggctgctaagaacctgtccgatgctatcctgctgtccgatatcctgagagtgaacactgagatcactaaggctccactgtccgcttccatgatcaagagatacgatgagcaccaccaagatctgactctgctgaaggctctggtgagacaacaactgccagagaagtacaaggagatcttcttcgatcaatccaagaacggatacgctggatacatcgatggaggagcttcccaagaggagttctacaagttcatcaagccaatcctggagaagatggatggaactgaggagctgctggtgaagctgaacagagaggatctgctgagaaagcaaagaactttcgataacggatccatcccacaccaaatccacctgggagagctgcacgctatcctgagaagacaagaggatttctacccattcctgaaggataacagagagaagatcgagaagatcctgactttcagaatcccatactacgtgggaccactggctagaggaaactccagattcgcttggatgactagaaagtccgaggagactatcactccatggaacttcgaggaggtggtggataagggagcttccgctcaatccttcatcgagagaatgactaacttcgataagaacctgccaaacgagaaggtgctgccaaagcactccctgctgtacgagtacttcactgtgtacaacgagctgactaaggtgaagtacgtgactgagggaatgagaaagccagctttcctgtccggagagcaaaagaaggctatcgtggatctgctgttcaagactaacagaaaggtgactgtgaagcaactgaaggaggattacttcaagaagatcgagtgcttcgattccgtggagatctccggagtggaggatagattcaacgcttccctgggaacttaccacgatctgctgaagatcatcaaggataaggatttcctggataacgaggagaacgaggatatcctggaggatatcgtgctgactctgactctgttcgaggatagagagatgatcgaggagagactgaagacttacgctcacctgttcgatgataaggtgatgaagcaactgaagagaagaagatacactggatggggaagactgtccagaaagctgatcaacggaatcagagataagcaatccggaaagactatcctggatttcctgaagtccgatggattcgctaacagaaacttcatgcaactgatccacgatgattccctgactttcaaggaggatatccaaaaggctcaagtgtccggacaaggagattccctgcacgagcacatcgctaacctggctggatccccagctatcaagaagggaatcctgcaaactgtgaaggtggtggatgagctggtgaaggtgatgggaagacacaagccagagaacatcgtgatcgagatggctagagagaaccaaactactcaaaagggacaaaagaactccagagagagaatgaagagaatcgaggagggaatcaaggagctgggatcccaaatcctgaaggagcacccagtggagaacactcaactgcaaaacgagaagctgtacctgtactacctgcaaaacggaagagatatgtacgtggatcaagagctggatatcaacagactgtccgattacgatgtggatcacatcgtgccacaatccttcctgaaggatgattccatcgataacaaggtgctgactagatccgataagaacagaggaaagtccgataacgtgccatccgaggaggtggtgaagaagatgaagaactactggagacaactgctgaacgctaagctgatcactcaaagaaagttcgataacctgactaaggctgagagaggaggactgtccgagctggataaggctggattcatcaagagacaactggtggagactagacaaatcactaagcacgtggctcaaatcctggattccagaatgaacactaagtacgatgagaacgataagctgatcagagaggtgaaggtgatcactctgaagtccaagctggtgtccgatttcagaaaggatttccaattctacaaggtgagagagatcaacaactaccaccacgctcacgatgcttacctgaacgctgtggtgggaactgctctgatcaagaagtacccaaagctggagtccgagttcgtgtacggagattacaaggtgtacgatgtgagaaagatgatcgctaagtccgagcaagagatcggaaaggctactgctaagtacttcttctactccaacatcatgaacttcttcaagactgagatcactctggctaacggagagatcagaaagagaccactgatcgagactaacggagagactggagagatcgtgtgggataagggaagagatttcgctactgtgagaaaggtgctgtccatgccacaagtgaacatcgtgaagaagactgaggtgcaaactggaggattctccaaggagtccatcctgccaaagagaaactccgataagctgatcgctagaaagaaggattgggatccaaagaagtacggaggattcgattccccaactgtggcttactccgtgctggtggtggctaaggtggagaagggaaagtccaagaagctgaagtccgtgaaggagctgctgggaatcactatcatggagagatcctccttcgagaagaacccaatcgatttcctggaggctaagggatacaaggaggtgaagaaggatctgatcatcaagctgccaaagtactccctgttcgagctggagaacggaagaaagagaatgctggcttccgctggagagctgcaaaagggaaacgagctggctctgccatccaagtacgtgaacttcctgtacctggcttcccactacgagaagctgaagggatccccagaggataacgagcaaaagcaactgttcgtggagcaacacaagcactacctggatgagatcatcgagcaaatctccgagttctccaagagagtgatcctggctgatgctaacctggataaggtgctgtccgcttacaacaagcacagagataagccaatcagagagcaagctgagaacatcatccacctgttcactctgactaacctgggagctccagctgctttcaagtacttcgatactactatcgatagaaagagatacacttccactaaggaggtgctggatgctactctgatccaccaatccatcactggactgtacgagactagaatcgatctgtcccaactgggaggagatctgcaaccaaagaagaagagaaaggtgggaggatgatctagaccagattaataTAACTAGTTCTAGAGCGGCCGCCCACCGCGGTGGAGCTCGAATTTCCCCGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAAAATATAGCGCGCAAACTAGGATAAATTATCGCGCGCGGTGTCATCTATGTTACTGaattcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattggctagagcagcttgccaacatggtggagcacgacactctcgtctactccaagaatatcaaagatacagtctcagaagaccaaagggctattgagacttttcaacaaagggtaatatcgggaaacctcctcggattccattgcccagctatctgtcacttcatcaaaaggacagtagaaaaggaaggtggcacctacaaatgccatcattgcgataaaggaaaggctatcgttcaagatgcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgataacatggtggagcacgacactctcgtctactccaagaatatcaaagatacagtctcagaagaccaaagggctattgagacttttcaacaaagggtaatatcgggaaacctcctcggattccattgcccagctatctgtcacttcatcaaaaggacagtagaaaaggaaggtggcacctacaaatgccatcattgcgataaaggaaaggctatcgttcaagatgcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagaccttcctctatataaggaagttcatttcatttggagaggacacgctgaaatcaccagtctctctctacaaatctatctctctcgagtctaccatgagcccagaacgacgcccggccgacatccgccgtgccaccgaggcggacatgccggcggtctgcaccatcgtcaaccactacatcgagacaagcacggtcaacttccgtaccgagccgcaggaaccgcaggagtggacggacgacctcgtccgtctgcgggagcgctatccctggctcgtcgccgaggtggacggcgaggtcgccggcatcgcctacgcgggcccctggaaggcacgcaacgcctacgactggacggccgagtcgaccgtgtacgtctccccccgccaccagcggacgggactgggctccacgctctacacccacctgctgaagtccctggaggcacagggcttcaagagcgtggtcgctgtcatcgggctgcccaacgacccgagcgtgcgcatgcacgaggcgctcggatatgccccccgcggcatgctgcgggcggccggcttcaagcacgggaactggcatgacgtgggtttctggcagctggacttcagcctgccggtaccgccccgtccggtcctgcccgtcaccgagatttgactcgagtttctccataataatgtgtgagtagttcccagataagggaattagggttcctatagggtttcgctcatgtgttgagcatataagaaacccttagtatgtatttgtatttgtaaaatacttctatcaataaaatttctaattcctaaaaccaaaatccagtactaaaatccagatcccccgaattaattcggcgttaattcagtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaatttgtttacaccacaatatatcctgccaccagccagccaacagctccccgaccggcagctcggcacaaaatcaccactcgatacaggcagcccatcagtccgggacggcgtcagcgggagagccgttgtaaggcggcagactttgctcatgttaccgatgctattcggaagaacggcaactaagctgccgggtttgaaacacggatgatctcgcggagggtagcatgttgattgtaacgatgacagagcgttgctgcctgtgatcaccgcggtttcaaaatcggctccgtcgatactatgttatacgccaactttgaaaacaactttgaaaaagctgttttctggtatttaaggttttagaatgcaaggaacagtgaattggagttcgtcttgttataattagcttcttggggtatctttaaatactgtagaaaagaggaaggaaataataaatggctaaaatgagaatatcaccggaattgaaaaaactgatcgaaaaataccgctgcgtaaaagatacggaaggaatgtctcctgctaaggtatataagctggtgggagaaaatgaaaacctatatttaaaaatgacggacagccggtataaagggaccacctatgatgtggaacgggaaaaggacatgatgctatggctggaaggaaagctgcctgttccaaaggtcctgcactttgaacggcatgatggctggagcaatctgctcatgagtgaggccgatggcgtcctttgctcggaagagtatgaagatgaacaaagccctgaaaagattatcgagctgtatgcggagtgcatcaggctctttcactccatcgacatatcggattgtccctatacgaatagcttagacagccgcttagccgaattggattacttactgaataacgatctggccgatgtggattgcgaaaactgggaagaagacactccatttaaagatccgcgcgagctgtatgattttttaaagacggaaaagcccgaagaggaacttgtcttttcccacggcgacctgggagacagcaacatctttgtgaaagatggcaaagtaagtggctttattgatcttgggagaagcggcagggcggacaagtggtatgacattgccttctgcgtccggtcgatcagggaggatatcggggaagaacagtatgtcgagctattttttgacttactggggatcaagcctgattgggagaaaataaaatattatattttactggatgaattgttttagtacctagaatgcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagggtgccttgatgtgggcgccggcggtcgagtggcgacggcgcggcttgtccgcgccctggtagattgcctggccgtaggccagccatttttgagcggccagcggccgcgataggccgacgcgaagcggcggggcgtagggagcgcagcgaccgaagggtaggcgctttttgcagctcttcggctgtgcgctggccagacagttatgcacaggccaggcgggttttaagagttttaataagttttaaagagttttaggcggaaaaatcgccttttttctcttttatatcagtcacttacatgtgtgaccggttcccaatgtacggctttgggttcccaatgtacgggttccggttcccaatgtacggctttgggttcccaatgtacgtgctatccacaggaaagagaccttttcgacctttttcccctgctagggcaatttgccctagcatctgctccgtacattaggaaccggcggatgcttcgccctcgatcaggttgcggtagcgcatgactaggatcgggccagcctgccccgcctcctccttcaaatcgtactccggcaggtcatttgacccgatcagcttgcgcacggtgaaacagaacttcttgaactctccggcgctgccactgcgttcgtagatcgtcttgaacaaccatctggcttctgccttgcctgcggcgcggcgtgccaggcggtagagaaaacggccgatgccgggatcgatcaaaaagtaatcggggtgaaccgtcagcacgtccgggttcttgccttctgtgatctcgcggtacatccaatcagctagctcgatctcgatgtactccggccgcccggtttcgctctttacgatcttgtagcggctaatcaaggcttcaccctcggataccgtcaccaggcggccgttcttggccttcttcgtacgctgcatggcaacgtgcgtggtgtttaaccgaatgcaggtttctaccaggtcgtctttctgctttccgccatcggctcgccggcagaacttgagtacgtccgcaacgtgtggacggaacacgcggccgggcttgtctcccttcccttcccggtatcggttcatggattcggttagatgggaaaccgccatcagtaccaggtcgtaatcccacacactggccatgccggccggccctgcggaaacctctacgtgcccgtctggaagctcgtagcggatcacctcgccagctcgtcggtcacgcttcgacagacggaaaacggccacgtccatgatgctgcgactatcgcgggtgcccacgtcatagagcatcggaacgaaaaaatctggttgctcgtcgcccttgggcggcttcctaatcgacggcgcaccggctgccggcggttgccgggattctttgcggattcgatcagcggccgcttgccacgattcaccggggcgtgcttctgcctcgatgcgttgccgctgggcggcctgcgcggccttcaacttctccaccaggtcatcacccagcgccgcgccgatttgtaccgggccggatggtttgcgaccgtcacgccgattcctcgggcttgggggttccagtgccattgcagggccggcagacaacccagccgcttacgcctggccaaccgcccgttcctccacacatggggcattccacggcgtcggtgcctggttgttcttgattttccatgccgcctcctttagccgctaaaattcatctactcatttattcatttgctcatttactctggtagctgcgcgatgtattcagatagcagctcggtaatggtcttgccttggcgtaccgcgtacatcttcagcttggtgtgatcctccgccggcaactgaaagttgacccgcttcatggctggcgtgtctgccaggctggccaacgttgcagccttgctgctgcgtgcgctcggacggccggcacttagcgtgtttgtgcttttgctcattttctctttacctcattaactcaaatgagttttgatttaatttcagcggccagcgcctggacctcgcgggcagcgtcgccctcgggttctgattcaagaacggttgtgccggcggcggcagtgcctgggtagctcacgcgctgcgtgatacgggactcaagaatgggcagctcgtacccggccagcgcctcggcaacctcaccgccgatgcgcgtgcctttgatcgcccgcgacacgacaaaggccgcttgtagccttccatccgtgacctcaatgcgctgcttaaccagctccaccaggtcggcggtggcccatatgtcgtaagggcttggctgcaccggaatcagcacgaagtcggctgccttgatcgcggacacagccaagtccgccgcctggggcgctccgtcgatcactacgaagtcgcgccggccgatggccttcacgtcgcggtcaatcgtcgggcggtcgatgccgacaacggttagcggttgatcttcccgcacggccgcccaatcgcgggcactgccctggggatcggaatcgactaacagaacatcggccccggcgagttgcagggcgcgggctagatgggttgcgatggtcgtcttgcctgacccgcctttctggttaagtacagcgataaccttcatgcgttccccttgcgtatttgtttatttactcatcgcatcatatacgcagcgaccgcatgacgcaagctgttttactcaaatacacatcacctttttagacggcggcgctcggtttcttcagcggccaagctggccggccaggccgccagcttggcatcagacaaaccggccaggatttcatgcagccgcacggttgagacgtgcgcgggcggctcgaacacgtacccggccgcgatcatctccgcctcgatctcttcggtaatgaaaaacggttcgtcctggccgtcctggtgcggtttcatgcttgttcctcttggcgttcattctcggcggccgccagggcgtcggcctcggtcaatgcgtcctcacggaaggcaccgcgccgcctggcctcggtgggcgtcacttcctcgctgcgctcaagtgcgcggtacagggtcgagcgatgcacgccaagcagtgcagccgcctctttcacggtgcggccttcctggtcgatcagctcgcgggcgtgcgcgatctgtgccggggtgagggtagggcgggggccaaacttcacgcctcgggccttggcggcctcgcgcccgctccgggtgcggtcgatgattagggaacgctcgaactcggcaatgccggcgaacacggtcaacaccatgcggccggccggcgtggtggtgtcggcccacggctctgccaggctacgcaggcccgcgccggcctcctggatgcgctcggcaatgtccagtaggtcgcgggtgctgcgggccaggcggtctagcctggtcactgtcacaacgtcgccagggcgtaggtggtcaagcatcctggccagctccgggcggtcgcgcctggtgccggtgatcttctcggaaaacagcttggtgcagccggccgcgtgcagttcggcccgttggttggtcaagtcctggtcgtcggtgctgacgcgggcatagcccagcaggccagcggcggcgctcttgttcatggcgtaatgtctccggttctagtcgcaagtattctactttatgcgactaaaacacgcgacaagaaaacgccaggaaaagggcagggcggcagcctgtcgcgtaacttaggacttgtgcgacatgtcgttttcagaagacggctgcactgaacgtcagaagccgactgcactatagcagcggaggggttggatcaaagtactttgatcccgaggggaaccctgtggttggcatg