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发货规格 |
1×10⁶ |
别名 |
HT clone H9; HT(H9); H 9; H-9 |
形态 |
淋巴母细胞 |
生长特性 |
悬浮 |
培养条件 |
RPMI-1640+10%FBS+1%P/S+2mM L-glutamine |
冻存条件 |
90%FBS+10%DMSO |
传代方式 |
离心 |
传代比例 |
维持细胞浓度在1x105~1x106个/ml |
换液频率 |
每周2~3次 |
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种属 |
人源 |
组织来源 |
血液 |
肿瘤类型 |
侵袭性皮肤T细胞淋巴瘤 |
供体信息 |
53年 男性 欧洲人 |
背景描述 |
H9细胞系来自一名患有塞扎里综合征的成年患者的HUT 78 T细胞系的克隆衍生物,表现出特殊的临床特征,使其与HIV研究高度相关。值得注意的是,它允许HIV-1复制,有助于从艾滋病患者和艾滋病前期患者的血液中分离和繁殖HIV-1 |
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蛋白表达 |
CD4+ |
同工酶 |
AK-1, 0, ES-D, 1, G6PD, B, GLO-I, 1, Me-2, 0, PGM1, 1, PGM3, 0 |
病毒 |
HIV-1 (HTLV-III) |
核型 |
Karyotypically, H9 is near triploid with a modal chromosome number of 69, ranging from 58 to 74, and exhibits a 2.5% frequency of higher ploidies. The cell line displays an extremely complex karyotype, with nearly 60% of the chromosomes per cell consisting of structurally altered marker chromosomes, including translocations such as t(3p4q), t(5q6q), t(5p6p), and deletions like del(7)(q32). Such chromosomal abnormalities contribute to the line?s unique genetic profile, influencing its behavior and response to viral infections. The absence of normal chromosomes N4, N5, N6, N7, N10, N13, N18, N19, N20, and X further distinguishes its genetic makeup |