|
|
|
| 发货规格 |
1×10⁶ |
| 别名 |
MDA_MB_231;MDA-MB231;MDA.MB.231;MDAMB231；MDA-MB231;MDAMB-231;MDAMB231;MDA-231;MDA-231P;MDA231;MDA231-BRE;MB231;MD Anderson-Metastatic Breast-231 |
| 形态 |
上皮细胞样 |
| 生长特性 |
贴壁 |
| 培养条件 |
DMEM/F12+5%马血清+10ug/ml胰岛素+20ng/ml表皮生长因子+100ng/ml霍乱毒素+0.5ug/mL氢化可的松 |
| 冻存条件 |
90%FBS+10%DMSO |
| 传代方式 |
胰酶消化 |
| 倍增时间 |
25~30h |
| 传代比例 |
1:2~1:4 |
| 换液频率 |
每周2~3次 |
| 生物安全等级 |
1 |
|
|
|
| 种属 |
人源 |
| 组织来源 |
乳腺 |
| 肿瘤类型 |
乳腺癌 |
| 供体信息 |
51岁白人女性 |
| 背景描述 |
成立于1973年，由一名51岁浸润性乳腺癌女性化疗后胸腔积液而成;广泛使用和广泛表征的细胞系;三阴性，并被描述为在裸鼠中具有致瘤性 |
|
|
|
| 蛋白表达 |
Epidermal growth factor(EGF)，expressed;transforming growth factoral pha(TGFalpha)，expressed |
| 受体表达 |
Epidermal growth factor (EGF)， expressed; transforming growth factor alpha (TGF alpha)， expressed |
| 同工酶 |
AK-1，1；ES-D，1；G6PD，B；GLO-I，2；Me-2，1-2；PGM1，1-2；PGM3，1 |
| 致癌基因 |
wnt7b |
| 致瘤性 |
Yes， in ALS treated BALB/c mice， forms poorly differentiated adenocarcinoma (grade III)；Yes， in nude mice， forms poorly differentiated adenocarcinoma (grade III) |
| 核型 |
The cell line is aneuploid female (modal number = 64， range = 52 to 68)， with chromosome counts in the near-triploid range. Normal chromosomes N8 and N15 were absent. Eleven stable rearranged marker chromosomes are noted as well as unassignable chromosomes in addition to the majority of autosomes that are trisomic. Many of the marker chromosomes are identical to those shown in the karyotype reported by K.L. Satya-Prakash， et al. |
STR鉴定STR位点信息STR鉴定图
