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| 发货规格 |
1×10⁶ |
| 别名 |
Siha; SIHA |
| 形态 |
上皮细胞样 |
| 生长特性 |
贴壁 |
| 培养条件 |
DMEM+10%FBS+1%P/S |
| 冻存条件 |
90%FBS+10%DMSO |
| 传代方式 |
胰酶消化 |
| 倍增时间 |
20h |
| 传代比例 |
1:3~1:8 |
| 换液频率 |
每周2~3次 |
| 生物安全等级 |
1 |
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| 种属 |
人源 |
| 组织来源 |
子宫颈 |
| 肿瘤类型 |
人瘤病毒相关性宫颈鳞状细胞癌 |
| 供体信息 |
55岁亚洲女性 |
| 背景描述 |
SiHa细胞是一种人宫颈鳞状细胞癌细胞系，几十年来已广泛用于研究。它们是从一名 55 岁的日本女性鳞状细胞癌患者的原发性子宫活检片段中分离出来的。由于其独特的遗传特征，该细胞系对研究宫颈癌和其他相关疾病的研究人员非常感兴趣。 |
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| 同工酶 |
AK-1，1；ES-D， 2；G6PD， B；GLO-I， 2；Me-2， 1；PGM1， 1；PGM3， 1 |
| 基因表达 |
p53+; pRB+ |
| 致瘤性 |
Yes， in nude mice; forms poorly differentiated epidermoid carcinoma (grade III) |
| 核型 |
modal number = 69; range = 51 to 72.This is a hypertriploid human cell line with the modal chromosome number of 71， occurring in 24% of cells. Most cells had the chromosome numbers distributed between 69 and 72. Polyploid cells occurred at 7.6%. Fifteen or more marker chromosomes were common to most cells. Among them are dup(2) (q22q31) and del(2) (q31) which probably resulted from the balanced translocation between two N2s. Most cells had two copies of del(2). M2 is an A3-sized acrocentric. M13 is a minute submetacentric with 1-3 copies per cell. Origins of both M2 and M13 are not identified. There were two copies of normal X chromosomes. N2 was absent and probably was replaced by dup(2) and del(2). |
STR鉴定STR位点信息STR鉴定图
