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发货规格 |
1×10⁶ |
别名 |
Ovcar-3; OVCAR 3; OVCAR.3; NIH:OVCAR-3; NIH:Ovcar-3; NIH:OVCAR3; NIH-OVCAR-3; NIHOVCAR3; OVCAR3; Ovcar3 |
形态 |
上皮细胞样 |
生长特性 |
贴壁 |
培养条件 |
RPMI-1640+20%FBS+1%P/S+0.01mg/ml牛胰岛素 |
冻存条件 |
90%FBS+10%DMSO |
传代方式 |
胰酶消化 |
倍增时间 |
35~64h |
传代比例 |
1:2~1:4 |
换液频率 |
每周2~3次 |
生物安全等级 |
1 |
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种属 |
人源 |
组织来源 |
卵巢 |
肿瘤类型 |
高级别卵巢浆液性腺癌 |
供体信息 |
60岁白人女性 |
背景描述 |
OVCAR-3 细胞是由一名 60 岁白种人女性卵巢进行性腺癌患者的恶性腹水建立的人类卵巢癌细胞系,对环磷酰胺、阿霉素和顺铂治疗无效。Ovcar 3 细胞用于广泛的研究,包括耐药性,特别是涉及 DNA 损伤反应生物标志物、同源重组修复以及整体细胞周期动力学、癌细胞生物学和基因表达研究的研究。OVCAR-3细胞其特征在于其高体外生长潜力和在免疫缺陷小鼠中形成肿瘤的能力。这些细胞表达卵巢癌的几种特征标志物,并已被广泛用于研究卵巢癌的生物学。 |
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受体表达 |
Androgen, estrogen, progesterone,Androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positive |
同工酶 |
G6PD, B, PGM1, 1, PGM3, 1, ES-D, 1, AK-1, 1, GLO-1, 1 |
致瘤性 |
Yes, in nude mice |
核型 |
The cell line is aneuploid human female, with chromosome counts in the sub to near-triploid range. Several normal chromosomes (N11, N13, N14, N15, N16, N17, and N22) are clearly under-represented. Many of these missing chromosomes are represented in the large number of cytogenetically altered chromosomes identified as marker chromosomes. In addition to the marker chromosomes, there are a large number of other structurally abnormal and unassignable chromosomes that are not recognized as markers. Random loss and gain of chromosomes from cell to cell are noted in the exact chromosome counts and in the analysis of the karyotypes. |