• 规格：20μl质粒
• 质粒图谱1： png/1-1F92GFRS60.png

质粒序列： 点击查看序列

LOCUS       Exported                7602 bp ds-DNA     circular SYN 27-SEP-2017

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    .

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 7602)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Sep 27, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..7602

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             115..693

                     /codon_start=1

                     /label=amyE-front

                     /translation="MFAKRFKTSLLPLFAGFLLLFHLVLAGPAAASAETANKSNELTAP

                     SIKSGTILHAWNWSFNTLKHNMKDIHDAGYTAIQTSPINQVKEGNQGDKSMSNWYWLYQ

                     PTSYQIGNRYLGTEQEFKEMCAAAEEYGIKVIVDAVINHTTSDYAAISNEVKSIPNWTH

                     GNTQIKNWSDRILEAYRIPAALANGDFRS"

     CDS             1039..1713

                     /codon_start=1

                     /label=Spe

                     /translation="MFGSGVESGLKPNSDLDFLVVVSEPLTDQSKEILIQKIRPISKKI

                     GDKSNLRYIELTIIIQQEMVPWNHPPKQEFIYGEWLQELYEQGYIPQKELNSDLTIMLY

                     QAKRKNKRIYGNYDLEELLPDIPFSDVRRAIMDSSEELIDNYQDDETNSILTLCRMILT

                     MDTGKIIPKDIAGNAVAESSPLEHRERILLAVRSYLGENIEWTNENVNLTINYLNNRLK

                     KL"

     CDS             1849..2868

                     /codon_start=1

                     /label=amyE-back

                     /translation="MSDDDIRLGWAVIASRSGSTPLFFSRPEGGGNGVRFPGKSQIGDR

                     GSALFEDQAITAVNRFHNVMAGQPEELSNPNGNNQIFMNQRGSHGVVLANAGSSSVSIN

                     TATKLPDGRYDNKAGAGSFQVNDGKLTGTINARSVAVLYPDDIAKAPHVFLENYKTGVT

                     HSFNDQLTITLRADANTTKAVYQINNGPDDRRLRMEINSQSEKEIQFGKTYTIMLKGTN

                     SDGVTRTEKYSFVKRDPASAKTIGYQNPNHWSQVNAYIYKHDGSRVIELTGSWPGKPMT

                     KNADGIYTLTLPADTDTTNAKVIFNNGSAQVPGQNQPGFDYVLNGLYNDSGLSGSLPH"

     CDS             4231..4965

                     /codon_start=1

                     /label=Erm

                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT

                     LELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPY

                     NISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPREYF

                     HPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID

                     DLNNISFEQFLSLFNSYKLFNK"

     rep_origin      complement(5773..6361)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(6532..7392)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

ORIGIN

        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga

       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt

      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat

      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt

      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg

      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg

      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg

      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa

      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat

      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca

      601 catggaaaca cacaaattaa aaactggtct gatcggatcc tagaagctta tcgaattcct

      661 gcagccctgg cgaatggcga ttttcgttcg tgaatacatg ttataataac tataactaat

      721 aacgtaacgt gactggcaag agatattttt aaaacaatga ataggtttac acttacttta

      781 gttttatgga aatgaaagat catatcatat ataatctaga ataaaattaa ctaaaataat

      841 tattatctag ataaaaaatt tagaagccaa tgaaatctat aaataaacta aattaagttt

      901 atttaattaa caactatgga tataaaatag gtactaatca aaatagtgag gaggatatat

      961 ttgaatacat acgaacaaat taataaagtg aaaaaaatac ttcggaaaca tttaaaaaat

     1021 aaccttattg gtacttacat gtttggatca ggagttgaga gtggactaaa accaaatagt

     1081 gatcttgact ttttagtcgt cgtatctgaa ccattgacag atcaaagtaa agaaatactt

     1141 atacaaaaaa ttagacctat ttcaaaaaaa ataggagata aaagcaactt acgatatatt

     1201 gaattaacaa ttattattca gcaagaaatg gtaccgtgga atcatcctcc caaacaagaa

     1261 tttatttatg gagaatggtt acaagagctt tatgaacaag gatacattcc tcagaaggaa

     1321 ttaaattcag atttaaccat aatgctttac caagcaaaac gaaaaaataa aagaatatac

     1381 ggaaattatg acttagagga attactacct gatattccat tttctgatgt gagaagagcc

     1441 attatggatt cgtcagagga attaatagat aattatcagg atgatgaaac caactctata

     1501 ttaactttat gccgtatgat tttaactatg gacacgggta aaatcatacc aaaagatatt

     1561 gcgggaaatg cagtggctga atcttctcca ttagaacata gggagagaat tttgttagca

     1621 gttcgtagtt atcttggaga gaatattgaa tggactaatg aaaatgtaaa tttaactata

     1681 aactatttaa ataacagatt aaaaaaatta taaaaaaatt gaaaaaatgg tggaaacact

     1741 tttttcaatt tttttgtttt attatttaat atttgggaaa tattcattct aattggtaat

     1801 cagattttag aaaacaataa acccttgcat agggggatct cgacatggat gagcgatgat

     1861 gatatccgtt taggctgggc ggtgatagct tctcgttcag gcagtacgcc tcttttcttt

     1921 tccagacctg agggaggcgg aaatggtgtg aggttcccgg ggaaaagcca aataggcgat

     1981 cgcgggagtg ctttatttga agatcaggct atcactgcgg tcaatagatt tcacaatgtg

     2041 atggctggac agcctgagga actctcgaac ccgaatggaa acaaccagat atttatgaat

     2101 cagcgcggct cacatggcgt tgtgctggca aatgcaggtt catcctctgt ctctatcaat

     2161 acggcaacaa aattgcctga tggcaggtat gacaataaag ctggagcggg ttcatttcaa

     2221 gtgaacgatg gtaaactgac aggcacgatc aatgccaggt ctgtagctgt gctttatcct

     2281 gatgatattg caaaagcgcc tcatgttttc cttgagaatt acaaaacagg tgtaacacat

     2341 tctttcaatg atcaactgac gattaccttg cgtgcagatg cgaatacaac aaaagccgtt

     2401 tatcaaatca ataatggacc agacgacagg cgtttaagga tggagatcaa ttcacaatcg

     2461 gaaaaggaga tccaatttgg caaaacatac accatcatgt taaaaggaac gaacagtgat

     2521 ggtgtaacga ggaccgagaa atacagtttt gttaaaagag atccagcgtc ggccaaaacc

     2581 atcggctatc aaaatccgaa tcattggagc caggtaaatg cttatatcta taaacatgat

     2641 gggagccgag taattgaatt gaccggatct tggcctggaa aaccaatgac taaaaatgca

     2701 gacggaattt acacgctgac gctgcctgcg gacacggata caaccaacgc aaaagtgatt

     2761 tttaataatg gcagcgccca agtgcccggt cagaatcagc ctggctttga ttacgtgcta

     2821 aatggtttat ataatgactc gggcttaagc ggttctcttc cccattgagg gcaaggctag

     2881 acgggactta ccgaaagaaa ccatcaatga tggtttcttt tttgttcata aatcagacaa

     2941 aacttttctc ttgcaaaagt ttgtgaagtg ttgcacaata taaatgtgaa atacttcaca

     3001 aacaaaaaga catcaaagag aaacataccc tgcaaggatg ctgatattgt ctgcatttgc

     3061 gccggagcaa accaaaaacc tggtgagaca cgccttgaat tagtagaaaa gaacttgaag

     3121 attttcaaag gcatcgttag tgaagtcatg gcgagcggat ttgacggcat tttcttagtc

     3181 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg

     3241 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt

     3301 caaggatcgc tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg

     3361 gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc

     3421 ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctactga

     3481 agtggatttc tttaagagct cctttaactt cctcaccagt agttgtatcg gtaccataag

     3541 tagaagcagc aacccaagta gctttaccag catccggttc aaccagcata gtaagaatct

     3601 tactggacat cggcagttct tcgaacagtg cgccaactac cagctctttc tgcagttcat

     3661 tcagggcacc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca

     3721 tcctgtctct tgatccatgg attacgcgtt aacccgggcc cgcggatgca tatgatcaga

     3781 tcctttaact ctggcaaccc tcaaaattga atgagacatg ctacacctcc ggataataaa

     3841 tatatataaa cgtatataga tttcataaag tctaacacac tagacttatt tacttcgtaa

     3901 ttaagtcgtt aaaccgtgtg ctctacgacc aaaactataa aacctttaag aactttcttt

     3961 ttttacaaga aaaaagaaat tagataaatc tctcatatct tttattcaat aatcgcatcc

     4021 gattgcagta taaatttaac gatcactcat catgttcata tttatcagag ctcgtgctat

     4081 aattatacta attttataag gaggaaaaaa tatgggcatt tttagtattt ttgtaatcag

     4141 cacagttcat tatcaaccaa acaaaaaata agtggttata atgaatcgtt aataagcaaa

     4201 attcatataa ccaaattaaa gagggttata atgaacgaga aaaatataaa acacagtcaa

     4261 aactttatta cttcaaaaca taatatagat aaaataatga caaatataag attaaatgaa

     4321 catgataata tctttgaaat cggctcagga aaaggccatt ttacccttga attagtaaag

     4381 aggtgtaatt tcgtaactgc cattgaaata gaccataaat tatgcaaaac tacagaaaat

     4441 aaacttgttg atcacgataa tttccaagtt ttaaacaagg atatattgca gtttaaattt

     4501 cctaaaaacc aatcctataa aatatatggt aatatacctt ataacataag tacggatata

     4561 atacgcaaaa ttgtttttga tagtatagct aatgagattt atttaatcgt ggaatacggg

     4621 tttgctaaaa gattattaaa tacaaaacgc tcattggcat tacttttaat ggcagaagtt

     4681 gatatttcta tattaagtat ggttccaaga gaatattttc atcctaaacc taaagtgaat

     4741 agctcactta tcagattaag tagaaaaaaa tcaagaatat cacacaaaga taaacaaaag

     4801 tataattatt tcgttatgaa atgggttaac aaagaataca agaaaatatt tacaaaaaat

     4861 caatttaaca attccttaaa acatgcagga attgacgatt taaacaatat tagctttgaa

     4921 caattcttat ctcttttcaa tagctataaa ttatttaata agtaagttaa gggatgcata

     4981 aactgcatcc cttaacttgt ttttcgtgtg cctatttttt gtgaatcgat tatgtctttt

     5041 gcgcagtcgg cttaaaccag ttttcgctgg tgcgaaaaaa gagtgtcttg tgacacctaa

     5101 attcaaaatc tatcggtcag atttataccg atttgatttt atatattctt gaataacata

     5161 cgccgagtta tcacataaaa gcgggaacca atcatcaaat ttaaacttca ttgcataatc

     5221 cattaaactc ttaaattcta cgattccttg ttcatcaata aactcaatca tttctttaat

     5281 taatttatat ctatctgttg ttgttttctt taataattca tcaacatcta caccgccata

     5341 aactatcata tcttcttttt gat