• 规格：20μl质粒
• 质粒图谱1： png/1-1F92F93Q4428.png

质粒序列： 点击查看序列

LOCUS       Exported                6178 bp ds-DNA     circular SYN 27-SEP-2017

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    .

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 6178)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Sep 27, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..6178

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        202..581

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        582..785

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     promoter        830..848

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     regulatory      927..936

                     /regulatory_class="other"

                     /note="vertebrate consensus sequence for strong initiation 

                     of translation (Kozak, 1987)"

     CDS             933..1652

                     /codon_start=1

                     /product="enhanced YFP"

                     /label=EYFP

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSQNVYLMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     polyA_signal    1745..1969

                     /label=bGH poly(A) signal

                     /note="bovine growth hormone polyadenylation signal"

     rep_origin      2015..2443

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        2457..2786

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      2637..2772

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             2853..3647

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=Neo

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    3821..3942

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(4393..4978)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(5149..6009)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(6010..6114)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt

       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct

      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg

      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta

      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg

      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc

      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa

      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca

      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta

      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt

      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg

      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca

      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca

      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc

      841 actataggga gacccaagct ggctagcgtt taaacttaag cttgcatgcc tgcaggtcga

      901 ctctagagga tccccgggta ccggtcgcca ccatggtgag caagggcgag gagctgttca

      961 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg

     1021 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca

     1081 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc

     1141 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc

     1201 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc

     1261 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg

     1321 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaga

     1381 acgtctatct gatggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc

     1441 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg

     1501 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca

     1561 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga

     1621 tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga attctgcaga

     1681 tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc

     1741 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg

     1801 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat

     1861 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag

     1921 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg

     1981 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc

     2041 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc

     2101 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct

     2161 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa

     2221 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc

     2281 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca

     2341 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat

     2401 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt

     2461 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc

     2521 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta

     2581 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc

     2641 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta

     2701 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct

     2761 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat

     2821 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag

     2881 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg

     2941 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca

     3001 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc

     3061 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg

     3121 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg

     3181 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta

     3241 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag

     3301 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac

     3361 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg

     3421 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg

     3481 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg

     3541 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg

     3601 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg

     3661 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc

     3721 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct

     3781 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta

     3841 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact